Gene description for MYLK3
Gene name myosin light chain kinase 3
Gene symbol MYLK3
Other names/aliases MLCK
MLCK2
caMLCK
Species Homo sapiens
 Database cross references - MYLK3
ExoCarta ExoCarta_91807
Vesiclepedia VP_91807
Entrez Gene 91807
HGNC 29826
MIM 612147
UniProt Q32MK0  
 MYLK3 identified in exosomes derived from the following tissue/cell type
Squamous carcinoma cells 20124223    
 Gene ontology annotations for MYLK3
Molecular Function
    calcium/calmodulin-dependent protein kinase activity GO:0004683 ISS
    myosin light chain kinase activity GO:0004687 IBA
    myosin light chain kinase activity GO:0004687 ISS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
Biological Process
    protein phosphorylation GO:0006468 ISS
    signal transduction GO:0007165 IBA
    sarcomere organization GO:0045214 IBA
    sarcomere organization GO:0045214 ISS
    sarcomerogenesis GO:0048769 ISS
    cardiac myofibril assembly GO:0055003 IBA
    cardiac myofibril assembly GO:0055003 ISS
    positive regulation of sarcomere organization GO:0060298 ISS
    cellular response to interleukin-1 GO:0071347 IMP
    positive regulation of tight junction disassembly GO:1905075 IMP
    positive regulation of membrane permeability GO:1905710 IMP
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISS
    cytosol GO:0005829 ISS
    actin cytoskeleton GO:0015629 IBA
 Experiment description of studies that identified MYLK3 in exosomes
1
Experiment ID 191
MISEV standards
Biophysical techniques
Alix|CD81|CD9
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MYLK3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PCDHB11  
Affinity Capture-MS Homo sapiens
2 ALB 213
Affinity Capture-MS Homo sapiens
3 CALM3 808
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 PCDHB5  
Affinity Capture-MS Homo sapiens
5 MAPK14 1432
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 OBSL1 23363
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 MYC  
Dosage Lethality Homo sapiens
8 MAPK12  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 HSP90AA1 3320
Affinity Capture-Luminescence Homo sapiens
View the network image/svg+xml
 Pathways in which MYLK3 is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here