Gene description for PI4K2B
Gene name phosphatidylinositol 4-kinase type 2 beta
Gene symbol PI4K2B
Other names/aliases PI4KIIB
PIK42B
Species Homo sapiens
 Database cross references - PI4K2B
ExoCarta ExoCarta_55300
Vesiclepedia VP_55300
Entrez Gene 55300
HGNC 18215
MIM 612101
UniProt Q8TCG2  
 PI4K2B identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for PI4K2B
Molecular Function
    1-phosphatidylinositol 4-kinase activity GO:0004430 IBA
    1-phosphatidylinositol 4-kinase activity GO:0004430 IDA
    1-phosphatidylinositol 4-kinase activity GO:0004430 TAS
    ATP binding GO:0005524 IEA
Biological Process
    phosphatidylinositol biosynthetic process GO:0006661 TAS
    Golgi organization GO:0007030 IBA
    endosome organization GO:0007032 IBA
    phosphatidylinositol phosphate biosynthetic process GO:0046854 IBA
    phosphatidylinositol phosphate biosynthetic process GO:0046854 IDA
Subcellular Localization
    Golgi membrane GO:0000139 IDA
    endosome GO:0005768 IBA
    endoplasmic reticulum membrane GO:0005789 IDA
    trans-Golgi network GO:0005802 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    membrane GO:0016020 HDA
    early endosome membrane GO:0031901 IDA
 Experiment description of studies that identified PI4K2B in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for PI4K2B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FTL 2512
Affinity Capture-MS Homo sapiens
2 TRIP13 9319
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 MAP2K2 5605
Affinity Capture-MS Homo sapiens
4 ZNF174  
Affinity Capture-MS Homo sapiens
5 TUBB 203068
Affinity Capture-MS Homo sapiens
6 SIRT6  
Affinity Capture-MS Homo sapiens
7 GMPPB 29925
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 FBXW5  
Affinity Capture-MS Homo sapiens
9 FAM167A  
Affinity Capture-MS Homo sapiens
10 GPR182  
Affinity Capture-MS Homo sapiens
11 BAG2 9532
Affinity Capture-MS Homo sapiens
12 HCST  
Affinity Capture-MS Homo sapiens
13 DNAJA2 10294
Affinity Capture-MS Homo sapiens
14 BBS7 55212
Affinity Capture-MS Homo sapiens
15 PLAUR 5329
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 MANSC1 54682
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 GFOD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 SPINT2 10653
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 EFNB1 1947
Affinity Capture-MS Homo sapiens
20 PDDC1 347862
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
22 FCER1A  
Affinity Capture-MS Homo sapiens
23 HSP90AA1 3320
Affinity Capture-Western Homo sapiens
24 B4GALT2  
Affinity Capture-MS Homo sapiens
25 MAGEA9  
Affinity Capture-MS Homo sapiens
26 PSME1 5720
Affinity Capture-MS Homo sapiens
27 HDAC10  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 CRYBB3  
Affinity Capture-MS Homo sapiens
29 KCNE3  
Affinity Capture-MS Homo sapiens
30 RCCD1  
Affinity Capture-MS Homo sapiens
31 INF2 64423
Affinity Capture-MS Homo sapiens
32 NPAS1  
Affinity Capture-MS Homo sapiens
33 GPR45  
Affinity Capture-MS Homo sapiens
34 LRRC61 65999
Affinity Capture-MS Homo sapiens
35 SSSCA1 10534
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 NAA11 84779
Affinity Capture-MS Homo sapiens
37 PPP4R1L  
Affinity Capture-MS Homo sapiens
38 WDR5B  
Affinity Capture-MS Homo sapiens
39 KBTBD4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 MAPK14 1432
Biochemical Activity Homo sapiens
41 SLC31A1 1317
Affinity Capture-MS Homo sapiens
42 AKT1 207
Two-hybrid Homo sapiens
43 HAVCR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 EFNB2 1948
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 TRMU  
Affinity Capture-MS Homo sapiens
46 SULT1C4  
Affinity Capture-MS Homo sapiens
47 ATG7 10533
Affinity Capture-MS Homo sapiens
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