Gene description for ADCY6
Gene name adenylate cyclase 6
Gene symbol ADCY6
Other names/aliases AC6
LCCS8
Species Homo sapiens
 Database cross references - ADCY6
ExoCarta ExoCarta_112
Vesiclepedia VP_112
Entrez Gene 112
HGNC 237
MIM 600294
UniProt O43306  
 ADCY6 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for ADCY6
Molecular Function
    adenylate cyclase activity GO:0004016 IBA
    adenylate cyclase activity GO:0004016 IDA
    protein kinase C binding GO:0005080 IDA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    protein kinase binding GO:0019901 ISS
    metal ion binding GO:0046872 IEA
Biological Process
    renal water homeostasis GO:0003091 ISS
    cAMP biosynthetic process GO:0006171 IDA
    adenylate cyclase-activating G protein-coupled receptor signaling pathway GO:0007189 IBA
    adenylate cyclase-activating G protein-coupled receptor signaling pathway GO:0007189 IDA
    adenylate cyclase-activating G protein-coupled receptor signaling pathway GO:0007189 ISS
    adenylate cyclase-activating dopamine receptor signaling pathway GO:0007191 IDA
    adenylate cyclase-activating serotonin receptor signaling pathway GO:0007192 IDA
    adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway GO:0007193 ISS
    adenylate cyclase-inhibiting serotonin receptor signaling pathway GO:0007198 ISS
    negative regulation of neuron projection development GO:0010977 ISS
    intracellular signal transduction GO:0035556 IEA
    negative regulation of urine volume GO:0035811 ISS
    cellular response to prostaglandin E stimulus GO:0071380 IDA
    cellular response to catecholamine stimulus GO:0071870 IDA
    blood vessel diameter maintenance GO:0097746 IMP
    cellular response to vasopressin GO:1904117 ISS
    cellular response to forskolin GO:1904322 IDA
Subcellular Localization
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 ISS
    plasma membrane GO:0005886 TAS
    cilium GO:0005929 IEA
    membrane GO:0016020 ISS
    stereocilium GO:0032420 IEA
 Experiment description of studies that identified ADCY6 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 211
MISEV standards
EM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
cytochrome c|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 212
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
Cytochrome C|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 231
MISEV standards
Biophysical techniques
Alix|CD63|CD9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 232
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 233
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ADCY6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RNF149 284996
Affinity Capture-MS Homo sapiens
2 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
3 NKAIN1  
Affinity Capture-MS Homo sapiens
4 ATXN1 6310
Two-hybrid Homo sapiens
5 KCNMB3  
Affinity Capture-MS Homo sapiens
6 SPAG16  
Affinity Capture-MS Homo sapiens
7 CX3CL1  
Affinity Capture-MS Homo sapiens
8 CHRNA4  
Affinity Capture-MS Homo sapiens
9 TTYH1  
Affinity Capture-MS Homo sapiens
10 OR10H2  
Affinity Capture-MS Homo sapiens
11 SLC39A4 55630
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 EXTL3 2137
Affinity Capture-MS Homo sapiens
13 TRIM66  
Affinity Capture-MS Homo sapiens
14 LGALS3 3958
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 MANSC1 54682
Affinity Capture-MS Homo sapiens
16 SYNE4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 TSPAN15 23555
Affinity Capture-MS Homo sapiens
18 CHRNA7  
Affinity Capture-Western Homo sapiens
19 B4GAT1 11041
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 MAMDC4  
Affinity Capture-MS Homo sapiens
21 TNFSF12 8742
Affinity Capture-MS Homo sapiens
22 DDX58 23586
Affinity Capture-RNA Homo sapiens
23 LPAR1 1902
Affinity Capture-MS Homo sapiens
24 Gnb1 14688
Two-hybrid Mus musculus
25 KCNE3  
Affinity Capture-MS Homo sapiens
26 CHRNB2  
Affinity Capture-MS Homo sapiens
27 GNAS 2778
Phenotypic Enhancement Homo sapiens
28 ANKMY2 57037
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 GDPD5 81544
Affinity Capture-MS Homo sapiens
30 ZNRF4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 LGALS9B 284194
Affinity Capture-MS Homo sapiens
32 GRIK5  
Affinity Capture-MS Homo sapiens
33 CLEC2B  
Affinity Capture-MS Homo sapiens
34 IPPK  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 TMEM74  
Affinity Capture-MS Homo sapiens
36 KRAS 3845
Proximity Label-MS Homo sapiens
37 YIPF3 25844
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 CD70 970
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 LGALS8 3964
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 SLC31A1 1317
Affinity Capture-MS Homo sapiens
41 NEUROG3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 CAV1 857
Affinity Capture-Western Homo sapiens
43 C3orf52  
Affinity Capture-MS Homo sapiens
44 TNFRSF10A 8797
Affinity Capture-MS Homo sapiens
45 GBAS 2631
Cross-Linking-MS (XL-MS) Homo sapiens
View the network image/svg+xml
 Pathways in which ADCY6 is involved
PathwayEvidenceSource
Activation of GABAB receptors IEA Reactome
Activation of GABAB receptors TAS Reactome
Adenylate cyclase activating pathway TAS Reactome
Adenylate cyclase inhibitory pathway IEA Reactome
Adenylate cyclase inhibitory pathway TAS Reactome
ADORA2B mediated anti-inflammatory cytokines production IEA Reactome
ADORA2B mediated anti-inflammatory cytokines production TAS Reactome
Adrenaline,noradrenaline inhibits insulin secretion TAS Reactome
Anti-inflammatory response favouring Leishmania parasite infection IEA Reactome
Anti-inflammatory response favouring Leishmania parasite infection TAS Reactome
Aquaporin-mediated transport TAS Reactome
Ca-dependent events TAS Reactome
Calmodulin induced events TAS Reactome
CaM pathway TAS Reactome
Cellular responses to mechanical stimuli TAS Reactome
Cellular responses to stimuli TAS Reactome
DAG and IP3 signaling TAS Reactome
Disease IEA Reactome
Disease TAS Reactome
FCGR3A-mediated IL10 synthesis TAS Reactome
G alpha (i) signalling events IEA Reactome
G alpha (i) signalling events TAS Reactome
G alpha (s) signalling events IEA Reactome
G alpha (s) signalling events TAS Reactome
G alpha (z) signalling events IEA Reactome
G alpha (z) signalling events TAS Reactome
G-protein mediated events IEA Reactome
G-protein mediated events TAS Reactome
GABA B receptor activation IEA Reactome
GABA B receptor activation TAS Reactome
GABA receptor activation IEA Reactome
GABA receptor activation TAS Reactome
Glucagon signaling in metabolic regulation TAS Reactome
Glucagon-like Peptide-1 (GLP1) regulates insulin secretion TAS Reactome
GPCR downstream signalling IEA Reactome
GPCR downstream signalling TAS Reactome
GPER1 signaling IEA Reactome
GPER1 signaling TAS Reactome
Hedgehog 'off' state TAS Reactome
High laminar flow shear stress activates signaling by PIEZO1 and PECAM1:CDH5:KDR in endothelial cells TAS Reactome
Infectious disease IEA Reactome
Infectious disease TAS Reactome
Integration of energy metabolism TAS Reactome
Intracellular signaling by second messengers TAS Reactome
Leishmania infection IEA Reactome
Leishmania infection TAS Reactome
Leishmania parasite growth and survival IEA Reactome
Leishmania parasite growth and survival TAS Reactome
Metabolism TAS Reactome
Neuronal System IEA Reactome
Neuronal System TAS Reactome
Neurotransmitter receptors and postsynaptic signal transmission IEA Reactome
Neurotransmitter receptors and postsynaptic signal transmission TAS Reactome
Opioid Signalling IEA Reactome
Opioid Signalling TAS Reactome
Parasitic Infection Pathways IEA Reactome
Parasitic Infection Pathways TAS Reactome
PKA activation TAS Reactome
PKA activation in glucagon signalling TAS Reactome
PKA-mediated phosphorylation of CREB TAS Reactome
PLC beta mediated events TAS Reactome
Regulation of insulin secretion TAS Reactome
Response of endothelial cells to shear stress TAS Reactome
Signal Transduction IEA Reactome
Signal Transduction TAS Reactome
Signaling by GPCR IEA Reactome
Signaling by GPCR TAS Reactome
Signaling by Hedgehog TAS Reactome
Transmission across Chemical Synapses IEA Reactome
Transmission across Chemical Synapses TAS Reactome
Transport of small molecules TAS Reactome
Vasopressin regulates renal water homeostasis via Aquaporins TAS Reactome





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