Gene description for ADCY6
Gene name adenylate cyclase 6
Gene symbol ADCY6
Other names/aliases AC6
LCCS8
Species Homo sapiens
 Database cross references - ADCY6
ExoCarta ExoCarta_112
Entrez Gene 112
HGNC 237
MIM 600294
UniProt O43306  
 ADCY6 identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for ADCY6
Molecular Function
    metal ion binding GO:0046872 IEA
    adenylate cyclase activity GO:0004016 IDA
    protein binding GO:0005515 IPI
    protein kinase binding GO:0019901 ISS
    ATP binding GO:0005524 IEA
Biological Process
    adenylate cyclase-activating G-protein coupled receptor signaling pathway GO:0007189 TAS
    adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway GO:0007193 TAS
    transmembrane transport GO:0055085 TAS
    energy reserve metabolic process GO:0006112 TAS
    negative regulation of neuron projection development GO:0010977 ISS
    cellular response to catecholamine stimulus GO:0071870 IDA
    small molecule metabolic process GO:0044281 TAS
    cellular response to prostaglandin E stimulus GO:0071380 IDA
    cAMP biosynthetic process GO:0006171 IDA
    cellular response to glucagon stimulus GO:0071377 TAS
    renal water homeostasis GO:0003091 TAS
    activation of phospholipase C activity GO:0007202 TAS
    synaptic transmission GO:0007268 TAS
    signal transduction GO:0007165 TAS
    activation of protein kinase A activity GO:0034199 TAS
    water transport GO:0006833 TAS
    intracellular signal transduction GO:0035556 IEA
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    epidermal growth factor receptor signaling pathway GO:0007173 TAS
    fibroblast growth factor receptor signaling pathway GO:0008543 TAS
    innate immune response GO:0045087 TAS
    dopamine receptor signaling pathway GO:0007212 IDA
Subcellular Localization
    plasma membrane GO:0005886 TAS
    integral component of membrane GO:0016021 IEA
    intracellular GO:0005622 IEA
    cilium GO:0005929 IEA
    membrane GO:0016020 ISS
 Experiment description of studies that identified ADCY6 in exosomes
1
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 231
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 232
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 233
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ADCY6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CHRNA7  
Affinity Capture-Western Homo sapiens
2 GNAS 2778
Phenotypic Enhancement Homo sapiens
View the network image/svg+xml



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