Gene description for ST3GAL2
Gene name ST3 beta-galactoside alpha-2,3-sialyltransferase 2
Gene symbol ST3GAL2
Other names/aliases Gal-NAc6S
SIAT4B
ST3GALII
ST3GalA.2
Species Homo sapiens
 Database cross references - ST3GAL2
ExoCarta ExoCarta_6483
Vesiclepedia VP_6483
Entrez Gene 6483
HGNC 10863
MIM 607188
UniProt Q16842  
 ST3GAL2 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for ST3GAL2
Molecular Function
    beta-galactoside (CMP) alpha-2,3-sialyltransferase activity GO:0003836 IBA
    beta-galactoside (CMP) alpha-2,3-sialyltransferase activity GO:0003836 IDA
    beta-galactoside (CMP) alpha-2,3-sialyltransferase activity GO:0003836 TAS
    sialyltransferase activity GO:0008373 TAS
    protein homodimerization activity GO:0042803 IDA
    beta-D-galactosyl-(1->3)-N-acetyl-beta-D-galactosaminide alpha-2,3- sialyltransferase GO:0047288 IDA
Biological Process
    protein glycosylation GO:0006486 IBA
    protein glycosylation GO:0006486 IDA
    glycosphingolipid biosynthetic process GO:0006688 TAS
    glycoprotein biosynthetic process GO:0009101 IDA
    glycolipid biosynthetic process GO:0009247 IDA
    oligosaccharide biosynthetic process GO:0009312 IDA
    ganglioside biosynthetic process via lactosylceramide GO:0010706 IBA
    ganglioside biosynthetic process via lactosylceramide GO:0010706 IDA
    globoside biosynthetic process via lactosylceramide GO:0010707 IDA
    O-glycan processing GO:0016266 TAS
    keratan sulfate biosynthetic process GO:0018146 TAS
    viral protein processing GO:0019082 TAS
    lipid glycosylation GO:0030259 IDA
    sialylation GO:0097503 IBA
    sialylation GO:0097503 IDA
    protein sialylation GO:1990743 IDA
Subcellular Localization
    Golgi membrane GO:0000139 IDA
    Golgi membrane GO:0000139 TAS
    extracellular region GO:0005576 IEA
    membrane GO:0016020 IBA
    Golgi cisterna membrane GO:0032580 IEA
 Experiment description of studies that identified ST3GAL2 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for ST3GAL2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 FKBP14  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 ENO2 2026
Two-hybrid Homo sapiens
4 CANX 821
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 CLEC12B  
Affinity Capture-MS Homo sapiens
6 OPALIN  
Affinity Capture-MS Homo sapiens
7 HSPA5 3309
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 IL13RA2 3598
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 SLC31A1 1317
Affinity Capture-MS Homo sapiens
10 FOXA3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 SLC2A2  
Affinity Capture-MS Homo sapiens
12 LMF2 91289
Affinity Capture-MS Homo sapiens
13 DPAGT1 1798
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 ASPHD2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 OPRL1 4987
Affinity Capture-MS Homo sapiens
16 GPR182  
Affinity Capture-MS Homo sapiens
17 TMPRSS11B 132724
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 LCTL  
Affinity Capture-MS Homo sapiens
19 DGCR2 9993
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which ST3GAL2 is involved
PathwayEvidenceSource
Asparagine N-linked glycosylation TAS Reactome
Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein TAS Reactome
Disease IEA Reactome
Disease TAS Reactome
Glycosaminoglycan metabolism TAS Reactome
Glycosphingolipid biosynthesis TAS Reactome
Glycosphingolipid metabolism TAS Reactome
Infectious disease IEA Reactome
Infectious disease TAS Reactome
Keratan sulfate biosynthesis TAS Reactome
Keratan sulfate/keratin metabolism TAS Reactome
Late SARS-CoV-2 Infection Events IEA Reactome
Late SARS-CoV-2 Infection Events TAS Reactome
Maturation of protein 3a TAS Reactome
Maturation of protein 3a IEA Reactome
Maturation of spike protein TAS Reactome
Metabolism TAS Reactome
Metabolism of carbohydrates TAS Reactome
Metabolism of lipids TAS Reactome
Metabolism of proteins TAS Reactome
O-linked glycosylation TAS Reactome
O-linked glycosylation of mucins TAS Reactome
Post-translational protein modification TAS Reactome
SARS-CoV Infections IEA Reactome
SARS-CoV Infections TAS Reactome
SARS-CoV-1 Infection TAS Reactome
SARS-CoV-2 Infection IEA Reactome
SARS-CoV-2 Infection TAS Reactome
Sialic acid metabolism TAS Reactome
Sphingolipid metabolism TAS Reactome
Synthesis of substrates in N-glycan biosythesis TAS Reactome
Termination of O-glycan biosynthesis TAS Reactome
Translation of Structural Proteins TAS Reactome
Translation of Structural Proteins IEA Reactome
Translation of Structural Proteins TAS Reactome
Viral Infection Pathways IEA Reactome
Viral Infection Pathways TAS Reactome





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