Gene description for DPAGT1
Gene name dolichyl-phosphate (UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase)
Gene symbol DPAGT1
Other names/aliases ALG7
CDG-Ij
CDG1J
CMS13
CMSTA2
D11S366
DGPT
DPAGT
DPAGT2
G1PT
GPT
UAGT
UGAT
Species Homo sapiens
 Database cross references - DPAGT1
ExoCarta ExoCarta_1798
Vesiclepedia VP_1798
Entrez Gene 1798
HGNC 2995
MIM 191350
UniProt Q9H3H5  
 DPAGT1 identified in sEVs derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for DPAGT1
Molecular Function
    UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase activity GO:0003975 IBA
    UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase activity GO:0003975 IDA
    UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase activity GO:0003975 IMP
    UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase activity GO:0003975 TAS
    UDP-N-acetylglucosamine-lysosomal-enzyme N-acetylglucosaminephosphotransferase activity GO:0003976 IDA
    protein binding GO:0005515 IPI
    glycosyltransferase activity GO:0016757 IEA
    identical protein binding GO:0042802 ISS
    metal ion binding GO:0046872 IEA
Biological Process
    UDP-N-acetylglucosamine metabolic process GO:0006047 IEA
    protein N-linked glycosylation GO:0006487 IMP
    dolichol-linked oligosaccharide biosynthetic process GO:0006488 IDA
    dolichol-linked oligosaccharide biosynthetic process GO:0006488 TAS
    dolichol metabolic process GO:0019348 IEA
Subcellular Localization
    endoplasmic reticulum membrane GO:0005789 ISS
    endoplasmic reticulum membrane GO:0005789 TAS
    membrane GO:0016020 IBA
    membrane GO:0016020 IDA
    membrane GO:0016020 ISS
    intracellular membrane-bounded organelle GO:0043231 IDA
 Experiment description of studies that identified DPAGT1 in sEVs
1
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for DPAGT1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ARMCX4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 RIT1 6016
Negative Genetic Homo sapiens
3 APEX1 328
Affinity Capture-RNA Homo sapiens
4 KRAS 3845
Synthetic Lethality Homo sapiens
Negative Genetic Homo sapiens
5 STX4 6810
Affinity Capture-MS Homo sapiens
6 CUL3 8452
Affinity Capture-MS Homo sapiens
7 LSM8 51691
Two-hybrid Homo sapiens
8 ADPGK 83440
Affinity Capture-MS Homo sapiens
9 OLFML3 56944
Affinity Capture-MS Homo sapiens
10 ST3GAL2 6483
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 CD1E  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 SCCPDH 51097
Affinity Capture-MS Homo sapiens
13 MFSD3  
Two-hybrid Homo sapiens
14 RAB21 23011
Affinity Capture-MS Homo sapiens
15 ALOX12B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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