ExoCarta: Gene summary

Credits | Contact

Gene description for SLC19A1
Gene name	solute carrier family 19 (folate transporter), member 1
Gene symbol	SLC19A1
Other names/aliases	CHMD FOLT IFC1 REFC RFC1
Species	Homo sapiens
Database cross references - SLC19A1
ExoCarta	ExoCarta_6573
Vesiclepedia	VP_6573
Entrez Gene	6573
HGNC	10937
MIM	600424
UniProt	P41440
SLC19A1 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells	23161513
Colorectal cancer cells	23161513
Colorectal cancer cells	23161513
Colorectal cancer cells	34887515
Ovarian cancer cells	23333927
Ovarian cancer cells	23333927

Gene ontology annotations for SLC19A1

Molecular Function
	protein binding	GO:0005515	IPI
	folic acid binding	GO:0005542	IBA
	organic anion transmembrane transporter activity	GO:0008514	ISS
	folic acid transmembrane transporter activity	GO:0008517	IDA
	folic acid transmembrane transporter activity	GO:0008517	ISS
	folic acid transmembrane transporter activity	GO:0008517	TAS
	folate:monoatomic anion antiporter activity	GO:0008518	IBA
	folate:monoatomic anion antiporter activity	GO:0008518	IDA
	folate:monoatomic anion antiporter activity	GO:0008518	IMP
	antiporter activity	GO:0015297	IDA
	methotrexate transmembrane transporter activity	GO:0015350	IDA
	xenobiotic transmembrane transporter activity	GO:0042910	ISS
	2',3'-cyclic GMP-AMP binding	GO:0061507	IDA
	cyclic-GMP-AMP transmembrane transporter activity	GO:0140360	IDA
Biological Process
	xenobiotic transmembrane transport	GO:0006855	ISS
	female pregnancy	GO:0007565	IEA
	organic anion transport	GO:0015711	ISS
	folic acid transport	GO:0015884	IMP
	folic acid transport	GO:0015884	ISS
	folic acid metabolic process	GO:0046655	TAS
	methotrexate transport	GO:0051958	IDA
	monoatomic anion transmembrane transport	GO:0098656	IEA
	folate transmembrane transport	GO:0098838	IBA
	folate transmembrane transport	GO:0098838	IDA
	cyclic-GMP-AMP transmembrane import across plasma membrane	GO:0140361	IDA
	positive regulation of cGAS/STING signaling pathway	GO:0141111	IMP
	transport across blood-brain barrier	GO:0150104	NAS
	transport across blood-brain barrier	GO:0150104	TAS
	folate import across plasma membrane	GO:1904447	IDA
Subcellular Localization
	plasma membrane	GO:0005886	IBA
	plasma membrane	GO:0005886	IDA
	plasma membrane	GO:0005886	TAS
	basolateral plasma membrane	GO:0016323	IBA
	basolateral plasma membrane	GO:0016323	IDA
	basolateral plasma membrane	GO:0016323	ISS
	apical plasma membrane	GO:0016324	IBA
	apical plasma membrane	GO:0016324	IDA
	apical plasma membrane	GO:0016324	ISS
	brush border membrane	GO:0031526	IEA

Experiment description of studies that identified SLC19A1 in exosomes

Experiment ID

207

MISEV standards
✔ EM	Biophysical techniques
✔ TSG101\|HSP70\|FLOT1	Enriched markers
✔ VDAC	Negative markers
✔ NTA	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

23161513

Organism

Homo sapiens

Experiment description

Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.

Authors

"Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."

Journal name

Mol Cell Proteomics

Publication year

2012

Sample

Colorectal cancer cells

Sample name

DKO-1

Isolation/purification methods

Differential centrifugation
Filtration

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry

Experiment ID

208

MISEV standards
✔ EM	Biophysical techniques
✔ TSG101\|HSP70\|FLOT1	Enriched markers
✔ VDAC	Negative markers
✔ NTA	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

23161513

Organism

Homo sapiens

Experiment description

Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.

Authors

"Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."

Journal name

Mol Cell Proteomics

Publication year

2012

Sample

Colorectal cancer cells

Sample name

Dks-8

Isolation/purification methods

Differential centrifugation
Filtration

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry

Experiment ID

209

MISEV standards
✘	Biophysical techniques
✔ TSG101\|HSP70\|FLOT1	Enriched markers
✔ VDAC	Negative markers
✔ NTA	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

23161513

Organism

Homo sapiens

Experiment description

Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.

Authors

"Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."

Journal name

Mol Cell Proteomics

Publication year

2012

Sample

Colorectal cancer cells

Sample name

DLD-1

Isolation/purification methods

Differential centrifugation
Filtration

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry

Experiment ID

1203

MISEV standards
✔ EM	Biophysical techniques
✔ SDCBP\|FLOT1\|CD9\|CD81\|CD63\|EPCAM\|GAPDH\|LAMP1\|TFRC\|CD151\|CD82\|LAMP2\|RAB35\|TSG101\|FLOT2\|RAB5B\|ICAM1\|RAB5A	Enriched markers
✘	Negative markers
✘	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

34887515

Organism

Homo sapiens

Experiment description

Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets

Authors

"Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."

Journal name

Nat Cell Biol

Publication year

2021

Sample

Colorectal cancer cells

Sample name

DiFi

Isolation/purification methods

Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation

Flotation density

Molecules identified in the study

Protein
miRNA

Methods used in the study

Western blotting
Mass spectrometry
RNA sequencing

Experiment ID

211

MISEV standards
✔ EM	Biophysical techniques
✔ TSG101\|Alix\|EpCAM\|TFRC	Enriched markers
✔ cytochrome c\|GOLGA2	Negative markers
✘	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

23333927

Organism

Homo sapiens

Experiment description

Characterization and proteomic analysis of ovarian cancer-derived exosomes.

Authors

"Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."

Journal name

J Proteomics

Publication year

2013

Sample

Ovarian cancer cells

Sample name

IGROV1

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient

Flotation density

1.09-1.15 g/mL

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry

Experiment ID

212

MISEV standards
✔ CEM	Biophysical techniques
✔ TSG101\|Alix\|EpCAM\|TFRC	Enriched markers
✔ Cytochrome C\|GOLGA2	Negative markers
✘	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

23333927

Organism

Homo sapiens

Experiment description

Characterization and proteomic analysis of ovarian cancer-derived exosomes.

Authors

"Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."

Journal name

J Proteomics

Publication year

2013

Sample

Ovarian cancer cells

Sample name

OVCAR-3

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient

Flotation density

1.09-1.15 g/mL

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry

Protein-protein interactions for SLC19A1

Protein Interactor

ExoCarta ID

Identification method

PubMed

Species

ATP2B3

492

Affinity Capture-MS

Homo sapiens

CD164L2

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

GJD3

Proximity Label-MS

Homo sapiens

RAB35

11021

Proximity Label-MS

Homo sapiens

PLEKHA4

57664

Affinity Capture-MS

Homo sapiens

ANKFY1

51479

Proximity Label-MS

Homo sapiens

NHLRC3

387921

Affinity Capture-MS

Homo sapiens

OCLN

100506658

Proximity Label-MS

Homo sapiens

CANX

821

Affinity Capture-MS

Homo sapiens

LAMTOR1

55004

Proximity Label-MS

Homo sapiens

MARCKS

4082

Proximity Label-MS

Homo sapiens

LAMP3

Proximity Label-MS

Homo sapiens

RAB5A

5868

Proximity Label-MS

Homo sapiens

LMAN1

3998

Proximity Label-MS

Homo sapiens

HCST

Affinity Capture-MS

Homo sapiens

ATG9A

79065

Affinity Capture-MS

Homo sapiens

EPB41L4A

64097

Proximity Label-MS

Homo sapiens

LAMP1

3916

Proximity Label-MS

Homo sapiens

VAMP3

9341

Affinity Capture-MS

Homo sapiens

GJA1

2697

Proximity Label-MS

Homo sapiens

LAMP2

3920

Proximity Label-MS

Homo sapiens

ARF6

382

Proximity Label-MS

Homo sapiens

ERGIC1

57222

Proximity Label-MS

Homo sapiens

C11orf52

91894

Proximity Label-MS

Homo sapiens

KRT26

353288

Two-hybrid

Homo sapiens

LYN

4067

Proximity Label-MS

Homo sapiens

RAB4A

5867

Proximity Label-MS

Homo sapiens

DNAJC5

80331

Proximity Label-MS

Homo sapiens

B3GAT1

Proximity Label-MS

Homo sapiens

RAB2A

5862

Proximity Label-MS

Homo sapiens

MLLT4

4301

Proximity Label-MS

Homo sapiens

DIRAS3

Proximity Label-MS

Homo sapiens

RAB9A

9367

Proximity Label-MS

Homo sapiens

APOB

338

Affinity Capture-MS

Homo sapiens

TNFRSF10C

Affinity Capture-MS

Homo sapiens

KRAS

3845

Proximity Label-MS

Homo sapiens

RAB5C

5878

Proximity Label-MS

Homo sapiens

RHOB

388

Proximity Label-MS

Homo sapiens

STX4

6810

Proximity Label-MS

Homo sapiens

CAV1

857

Proximity Label-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

ATP2A3

489

Affinity Capture-MS

Homo sapiens

FLOT1

10211

Proximity Label-MS

Homo sapiens

STX6

10228

Proximity Label-MS

Homo sapiens

View the network

Pathways in which SLC19A1 is involved

Pathway	Evidence	Source
Metabolism	TAS	Reactome
Metabolism of folate and pterines	TAS	Reactome
Metabolism of vitamins and cofactors	TAS	Reactome
Metabolism of water-soluble vitamins and cofactors	TAS	Reactome

Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here