Gene description for SLC19A1
Gene name solute carrier family 19 (folate transporter), member 1
Gene symbol SLC19A1
Other names/aliases CHMD
FOLT
IFC1
REFC
RFC1
Species Homo sapiens
 Database cross references - SLC19A1
ExoCarta ExoCarta_6573
Vesiclepedia VP_6573
Entrez Gene 6573
HGNC 10937
MIM 600424
UniProt P41440  
 SLC19A1 identified in sEVs derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 34887515    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for SLC19A1
Molecular Function
    protein binding GO:0005515 IPI
    folic acid binding GO:0005542 IBA
    organic anion transmembrane transporter activity GO:0008514 ISS
    folic acid transmembrane transporter activity GO:0008517 IDA
    folic acid transmembrane transporter activity GO:0008517 ISS
    folic acid transmembrane transporter activity GO:0008517 TAS
    folate:monoatomic anion antiporter activity GO:0008518 IBA
    folate:monoatomic anion antiporter activity GO:0008518 IDA
    folate:monoatomic anion antiporter activity GO:0008518 IMP
    antiporter activity GO:0015297 IDA
    methotrexate transmembrane transporter activity GO:0015350 IDA
    xenobiotic transmembrane transporter activity GO:0042910 ISS
    2',3'-cyclic GMP-AMP binding GO:0061507 IDA
    cyclic-GMP-AMP transmembrane transporter activity GO:0140360 IDA
Biological Process
    xenobiotic transmembrane transport GO:0006855 ISS
    female pregnancy GO:0007565 IEA
    organic anion transport GO:0015711 ISS
    folic acid transport GO:0015884 IMP
    folic acid transport GO:0015884 ISS
    folic acid metabolic process GO:0046655 TAS
    methotrexate transport GO:0051958 IDA
    monoatomic anion transmembrane transport GO:0098656 IEA
    folate transmembrane transport GO:0098838 IBA
    folate transmembrane transport GO:0098838 IDA
    cyclic-GMP-AMP transmembrane import across plasma membrane GO:0140361 IDA
    positive regulation of cGAS/STING signaling pathway GO:0141111 IMP
    transport across blood-brain barrier GO:0150104 NAS
    transport across blood-brain barrier GO:0150104 TAS
    folate import across plasma membrane GO:1904447 IDA
Subcellular Localization
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    basolateral plasma membrane GO:0016323 IBA
    basolateral plasma membrane GO:0016323 IDA
    basolateral plasma membrane GO:0016323 ISS
    apical plasma membrane GO:0016324 IBA
    apical plasma membrane GO:0016324 IDA
    apical plasma membrane GO:0016324 ISS
    brush border membrane GO:0031526 IEA
 Experiment description of studies that identified SLC19A1 in sEVs
1
Experiment ID 207
MISEV standards
EM
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
MISEV standards
EM
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
MISEV standards
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 1203
MISEV standards
EM
Biophysical techniques
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
5
Experiment ID 211
MISEV standards
EM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
cytochrome c|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 212
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
Cytochrome C|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SLC19A1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ATP2B3 492
Affinity Capture-MS Homo sapiens
2 CD164L2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 GJD3  
Proximity Label-MS Homo sapiens
4 RAB35 11021
Proximity Label-MS Homo sapiens
5 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
6 ANKFY1 51479
Proximity Label-MS Homo sapiens
7 NHLRC3 387921
Affinity Capture-MS Homo sapiens
8 OCLN 100506658
Proximity Label-MS Homo sapiens
9 CANX 821
Affinity Capture-MS Homo sapiens
10 LAMTOR1 55004
Proximity Label-MS Homo sapiens
11 MARCKS 4082
Proximity Label-MS Homo sapiens
12 LAMP3  
Proximity Label-MS Homo sapiens
13 RAB5A 5868
Proximity Label-MS Homo sapiens
14 LMAN1 3998
Proximity Label-MS Homo sapiens
15 HCST  
Affinity Capture-MS Homo sapiens
16 ATG9A 79065
Affinity Capture-MS Homo sapiens
17 EPB41L4A 64097
Proximity Label-MS Homo sapiens
18 LAMP1 3916
Proximity Label-MS Homo sapiens
19 VAMP3 9341
Affinity Capture-MS Homo sapiens
20 GJA1 2697
Proximity Label-MS Homo sapiens
21 LAMP2 3920
Proximity Label-MS Homo sapiens
22 ARF6 382
Proximity Label-MS Homo sapiens
23 ERGIC1 57222
Proximity Label-MS Homo sapiens
24 C11orf52 91894
Proximity Label-MS Homo sapiens
25 KRT26 353288
Two-hybrid Homo sapiens
26 LYN 4067
Proximity Label-MS Homo sapiens
27 RAB4A 5867
Proximity Label-MS Homo sapiens
28 DNAJC5 80331
Proximity Label-MS Homo sapiens
29 B3GAT1  
Proximity Label-MS Homo sapiens
30 RAB2A 5862
Proximity Label-MS Homo sapiens
31 MLLT4 4301
Proximity Label-MS Homo sapiens
32 DIRAS3  
Proximity Label-MS Homo sapiens
33 RAB9A 9367
Proximity Label-MS Homo sapiens
34 APOB 338
Affinity Capture-MS Homo sapiens
35 TNFRSF10C  
Affinity Capture-MS Homo sapiens
36 KRAS 3845
Proximity Label-MS Homo sapiens
37 RAB5C 5878
Proximity Label-MS Homo sapiens
38 RHOB 388
Proximity Label-MS Homo sapiens
39 STX4 6810
Proximity Label-MS Homo sapiens
40 CAV1 857
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 ATP2A3 489
Affinity Capture-MS Homo sapiens
42 FLOT1 10211
Proximity Label-MS Homo sapiens
43 STX6 10228
Proximity Label-MS Homo sapiens
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