Gene description for PODXL2
Gene name podocalyxin-like 2
Gene symbol PODXL2
Other names/aliases EG
PODLX2
Species Homo sapiens
 Database cross references - PODXL2
ExoCarta ExoCarta_50512
Vesiclepedia VP_50512
Entrez Gene 50512
HGNC 17936
UniProt Q9NZ53  
 PODXL2 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 34887515    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for PODXL2
Molecular Function
    protein binding GO:0005515 IPI
    glycosaminoglycan binding GO:0005539 TAS
Biological Process
    leukocyte tethering or rolling GO:0050901 IBA
    leukocyte tethering or rolling GO:0050901 IDA
Subcellular Localization
    Golgi lumen GO:0005796 TAS
    plasma membrane GO:0005886 IEA
 Experiment description of studies that identified PODXL2 in exosomes
1
Experiment ID 1203
MISEV standards
EM
Biophysical techniques
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
2
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for PODXL2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 VCAM1 7412
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 UFSP2 55325
Affinity Capture-MS Homo sapiens
3 SLC39A12  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 CRLF2 64109
Affinity Capture-MS Homo sapiens
5 LMAN2 10960
Affinity Capture-MS Homo sapiens
6 RAET1E  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 MECP2 4204
Affinity Capture-MS Homo sapiens
8 PCDHGB1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 PRPH2 5961
Affinity Capture-MS Homo sapiens
10 GALNT2 2590
Affinity Capture-MS Homo sapiens
11 FCGRT 2217
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 TNFSF8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 CD1B 910
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 TMEM30B 161291
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 SLC9A3R2 9351
Affinity Capture-MS Homo sapiens
16 LAMP2 3920
Proximity Label-MS Homo sapiens
17 TMX1 81542
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 ARF6 382
Proximity Label-MS Homo sapiens
19 CEACAM21  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 LRRN4CL 221091
Affinity Capture-MS Homo sapiens
21 TNFSF18 8995
Affinity Capture-MS Homo sapiens
22 SMIM5  
Affinity Capture-MS Homo sapiens
23 PDCD1LG2 80380
Affinity Capture-MS Homo sapiens
24 KIAA1429 25962
Affinity Capture-MS Homo sapiens
25 PODXL2 50512
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
26 MOV10 4343
Affinity Capture-RNA Homo sapiens
27 CTLA4  
Affinity Capture-MS Homo sapiens
28 RAB11A 8766
Proximity Label-MS Homo sapiens
29 SLC9A3R1 9368
Affinity Capture-MS Homo sapiens
30 RHPN1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 TGFA  
Affinity Capture-MS Homo sapiens
32 KRAS 3845
Proximity Label-MS Homo sapiens
33 SLC47A1  
Affinity Capture-MS Homo sapiens
34 TPBG 7162
Affinity Capture-MS Homo sapiens
35 RYK 6259
Affinity Capture-MS Homo sapiens
36 CD63 967
Affinity Capture-MS Homo sapiens
37 SELL 6402
Reconstituted Complex Homo sapiens
38 B3GALT5  
Affinity Capture-MS Homo sapiens
39 ITFG3 83986
Affinity Capture-MS Homo sapiens
40 CLRN2  
Affinity Capture-MS Homo sapiens
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