Gene description for WASF1
Gene name WAS protein family, member 1
Gene symbol WASF1
Other names/aliases SCAR1
WAVE
WAVE1
Species Homo sapiens
 Database cross references - WASF1
ExoCarta ExoCarta_8936
Entrez Gene 8936
HGNC 12732
MIM 605035
UniProt Q92558  
 WASF1 identified in exosomes derived from the following tissue/cell type
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for WASF1
Molecular Function
    actin binding GO:0003779 IEA
    protein complex binding GO:0032403 IMP
    protein binding GO:0005515 IPI
    Rac GTPase binding GO:0048365 IMP
Biological Process
    Rac protein signal transduction GO:0016601 IMP
    movement of cell or subcellular component GO:0006928 TAS
    lamellipodium morphogenesis GO:0072673 IEA
    positive regulation of Arp2/3 complex-mediated actin nucleation GO:2000601 IMP
    actin filament polymerization GO:0030041 TAS
    protein complex assembly GO:0006461 NAS
Subcellular Localization
    lamellipodium GO:0030027 IEA
    actin cytoskeleton GO:0015629 TAS
    cytoskeleton GO:0005856 TAS
    mitochondrial outer membrane GO:0005741 IEA
    SCAR complex GO:0031209 IMP
    cell junction GO:0030054 IEA
    synapse GO:0045202 IEA
 Experiment description of studies that identified WASF1 in exosomes
1
Experiment ID 231
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 232
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 233
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for WASF1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PSTPIP1 9051
Invivo Homo sapiens
Invitro Homo sapiens
2 SRGAP3  
Invivo Homo sapiens
Invitro Homo sapiens
3 ARHGEF2 9181
Affinity Capture-Western Homo sapiens
4 DNMBP  
Affinity Capture-MS Homo sapiens
5 PFN1 5216
Affinity Capture-Western Homo sapiens
Far Western Homo sapiens
View the network image/svg+xml



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