Gene description for CLIC2
Gene name chloride intracellular channel 2
Gene symbol CLIC2
Other names/aliases CLIC2b
MRXS32
XAP121
Species Homo sapiens
 Database cross references - CLIC2
ExoCarta ExoCarta_1193
Vesiclepedia VP_1193
Entrez Gene 1193
HGNC 2063
MIM 300138
UniProt O15247  
 CLIC2 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for CLIC2
Molecular Function
    glutathione transferase activity GO:0004364 IDA
    glutathione peroxidase activity GO:0004602 IDA
    chloride channel activity GO:0005254 IBA
    protein binding GO:0005515 IPI
Biological Process
    chloride transport GO:0006821 IBA
    signal transduction GO:0007165 TAS
    regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum GO:0010880 IDA
    regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion GO:0010881 IC
    positive regulation of binding GO:0051099 IDA
    negative regulation of ryanodine-sensitive calcium-release channel activity GO:0060315 IDA
    cellular oxidant detoxification GO:0098869 IEA
    chloride transmembrane transport GO:1902476 IEA
Subcellular Localization
    nucleus GO:0005634 TAS
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 TAS
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 IDA
    membrane GO:0016020 IBA
    chloride channel complex GO:0034707 IEA
 Experiment description of studies that identified CLIC2 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CLIC2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TPRN 286262
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
2 FN1 2335
Affinity Capture-MS Homo sapiens
3 TRAPPC2 6399
Two-hybrid Homo sapiens
4 CLIC5 53405
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 SBDS 51119
Affinity Capture-MS Homo sapiens
6 LYRM2  
Co-fractionation Homo sapiens
7 C15orf40  
Affinity Capture-MS Homo sapiens
8 LHPP 64077
Affinity Capture-MS Homo sapiens
9 RPP14  
Affinity Capture-MS Homo sapiens
10 RPL35 11224
Affinity Capture-MS Homo sapiens
11 CDO1  
Co-fractionation Homo sapiens
12 NUF2  
Co-fractionation Homo sapiens
13 NEDD4 4734
Biochemical Activity Homo sapiens
14 UBL7  
Co-fractionation Homo sapiens
15 CLIC4 25932
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 SAP30 8819
Affinity Capture-MS Homo sapiens
17 TXNDC17 84817
Affinity Capture-MS Homo sapiens
18 KIF14 9928
Affinity Capture-MS Homo sapiens
19 NEDD4L 23327
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
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