Gene description for MYO9B
Gene name myosin IXB
Gene symbol MYO9B
Other names/aliases CELIAC4
MYR5
Species Homo sapiens
 Database cross references - MYO9B
ExoCarta ExoCarta_4650
Vesiclepedia VP_4650
Entrez Gene 4650
HGNC 7609
MIM 602129
UniProt Q13459  
 MYO9B identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for MYO9B
Molecular Function
    microfilament motor activity GO:0000146 IBA
    microfilament motor activity GO:0000146 IDA
    actin binding GO:0003779 IDA
    GTPase activator activity GO:0005096 IDA
    GTPase activator activity GO:0005096 TAS
    protein binding GO:0005515 IPI
    calmodulin binding GO:0005516 IDA
    ATP binding GO:0005524 IBA
    ATP binding GO:0005524 IDA
    ATP hydrolysis activity GO:0016887 IBA
    small GTPase binding GO:0031267 IPI
    protein homodimerization activity GO:0042803 IDA
    ADP binding GO:0043531 IDA
    metal ion binding GO:0046872 IEA
    Roundabout binding GO:0048495 IPI
    actin filament binding GO:0051015 IBA
Biological Process
    Rho protein signal transduction GO:0007266 IC
    actin filament-based movement GO:0030048 IBA
    actin filament-based movement GO:0030048 IDA
    actin filament-based movement GO:0030048 TAS
    ARF protein signal transduction GO:0032011 IDA
    regulation of Rho protein signal transduction GO:0035023 IMP
    Roundabout signaling pathway GO:0035385 IDA
    regulation of small GTPase mediated signal transduction GO:0051056 TAS
    lamellipodium morphogenesis GO:0072673 IBA
Subcellular Localization
    ruffle GO:0001726 IBA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    actin filament GO:0005884 IBA
    actin filament GO:0005884 IDA
    cell cortex GO:0005938 IDA
    actin cytoskeleton GO:0015629 TAS
    membrane GO:0016020 HDA
    myosin complex GO:0016459 IEA
    lamellipodium GO:0030027 IBA
    perinuclear region of cytoplasm GO:0048471 IDA
    postsynaptic actin cytoskeleton GO:0098871 IDA
    postsynaptic actin cytoskeleton GO:0098871 IMP
 Experiment description of studies that identified MYO9B in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
12
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MYO9B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CITED1 4435
Affinity Capture-MS Homo sapiens
2 SOX2  
Affinity Capture-MS Homo sapiens
3 NAP1L4 4676
Affinity Capture-MS Homo sapiens
4 GRK5 2869
Affinity Capture-MS Homo sapiens
5 FAM131B  
Affinity Capture-MS Homo sapiens
6 LCN15  
Affinity Capture-MS Homo sapiens
7 PKN2 5586
Two-hybrid Homo sapiens
8 CA6 765
Affinity Capture-MS Homo sapiens
9 VPS13A 23230
Cross-Linking-MS (XL-MS) Homo sapiens
10 CUL3 8452
Affinity Capture-MS Homo sapiens
11 RPA2 6118
Proximity Label-MS Homo sapiens
12 MYL6 4637
Affinity Capture-MS Homo sapiens
13 TMOD4  
Affinity Capture-MS Homo sapiens
14 C1orf54  
Affinity Capture-MS Homo sapiens
15 PFN1 5216
Proximity Label-MS Homo sapiens
16 CALM2 805
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 CDH5  
Affinity Capture-MS Homo sapiens
18 DKK3 27122
Affinity Capture-MS Homo sapiens
19 FAM96A  
Affinity Capture-MS Homo sapiens
20 MEX3A  
Affinity Capture-RNA Homo sapiens
21 C14orf169  
Affinity Capture-MS Homo sapiens
22 SSSCA1 10534
Affinity Capture-MS Homo sapiens
23 NEUROG3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 FOXG1  
Affinity Capture-MS Homo sapiens
25 MCC 4163
Affinity Capture-MS Homo sapiens
26 HSP90AB1 3326
Cross-Linking-MS (XL-MS) Homo sapiens
27 GPR182  
Affinity Capture-MS Homo sapiens
28 BPNT1 10380
Affinity Capture-MS Homo sapiens
29 MAPRE3  
Proximity Label-MS Homo sapiens
30 FOXL1  
Affinity Capture-MS Homo sapiens
31 KIF23 9493
Affinity Capture-MS Homo sapiens
32 MAGEA9  
Affinity Capture-MS Homo sapiens
33 FAXC  
Affinity Capture-MS Homo sapiens
34 RBM33  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 EIF2B5 8893
Affinity Capture-MS Homo sapiens
36 GPRASP2  
Affinity Capture-MS Homo sapiens
37 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
38 DCAF8 50717
Affinity Capture-MS Homo sapiens
39 TRIM52  
Affinity Capture-MS Homo sapiens
40 SKAP1 8631
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 VRK1 7443
Affinity Capture-MS Homo sapiens
42 ACTG1 71
Affinity Capture-MS Homo sapiens
43 PJA1  
Affinity Capture-MS Homo sapiens
44 MYL6B 140465
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 PCBP1 5093
Affinity Capture-MS Homo sapiens
46 TARBP2  
Affinity Capture-MS Homo sapiens
47 EMILIN1 11117
Affinity Capture-MS Homo sapiens
48 CALM1 801
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
49 MYC  
Affinity Capture-MS Homo sapiens
50 DQX1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 PARK2  
Affinity Capture-MS Homo sapiens
52 NTRK1 4914
Affinity Capture-MS Homo sapiens
53 MCM2 4171
Affinity Capture-MS Homo sapiens
54 HIST1H1C 3006
Cross-Linking-MS (XL-MS) Homo sapiens
55 ZCCHC10  
Affinity Capture-MS Homo sapiens
56 IFT43  
Affinity Capture-MS Homo sapiens
57 CIT 11113
Affinity Capture-MS Homo sapiens
58 RPA3 6119
Proximity Label-MS Homo sapiens
59 RAB2A 5862
Cross-Linking-MS (XL-MS) Homo sapiens
60 FBXW7  
Affinity Capture-MS Homo sapiens
61 SENP3 26168
Affinity Capture-MS Homo sapiens
62 ACTB 60
Proximity Label-MS Homo sapiens
63 EID1  
Affinity Capture-MS Homo sapiens
64 NPM1 4869
Affinity Capture-MS Homo sapiens
65 CALM3 808
Affinity Capture-MS Homo sapiens
66 LRRC41 10489
Two-hybrid Homo sapiens
67 HECTD1 25831
Affinity Capture-MS Homo sapiens
68 NEFM 4741
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
69 NAP1L1 4673
Affinity Capture-MS Homo sapiens
70 MOAP1  
Affinity Capture-MS Homo sapiens
71 SDF4 51150
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 HLA-DPA1  
Affinity Capture-MS Homo sapiens
73 BRD3 8019
Affinity Capture-MS Homo sapiens
74 PPM1G 5496
Affinity Capture-MS Homo sapiens
75 RHOB 388
Proximity Label-MS Homo sapiens
76 HSPB8 26353
Affinity Capture-MS Homo sapiens
77 DICER1  
Affinity Capture-MS Homo sapiens
78 ECD  
Affinity Capture-MS Homo sapiens
79 CCDC8  
Affinity Capture-MS Homo sapiens
80 HIF1A 3091
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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