Gene description for B3GALT6
Gene name UDP-Gal:betaGal beta 1,3-galactosyltransferase polypeptide 6
Gene symbol B3GALT6
Other names/aliases EDSP2
SEMDJL1
beta3GalT6
Species Homo sapiens
 Database cross references - B3GALT6
ExoCarta ExoCarta_126792
Entrez Gene 126792
HGNC 17978
MIM 615291
UniProt Q96L58  
 B3GALT6 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
 Gene ontology annotations for B3GALT6
Molecular Function
    UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase activity GO:0008499 IEA
    galactosylxylosylprotein 3-beta-galactosyltransferase activity GO:0047220 IEA
    UDP-galactosyltransferase activity GO:0035250 IDA
Biological Process
    glycosaminoglycan metabolic process GO:0030203 TAS
    protein glycosylation GO:0006486 IEA
    glycosaminoglycan biosynthetic process GO:0006024 IMP
    small molecule metabolic process GO:0044281 TAS
    heparan sulfate proteoglycan biosynthetic process GO:0015012 IEA
    carbohydrate metabolic process GO:0005975 TAS
    chondroitin sulfate biosynthetic process GO:0030206 IEA
    chondroitin sulfate metabolic process GO:0030204 TAS
Subcellular Localization
    membrane GO:0016020 IDA
    integral component of membrane GO:0016021 IEA
    Golgi cisterna membrane GO:0032580 IEA
    Golgi membrane GO:0000139 TAS
    Golgi medial cisterna GO:0005797 IDA
 Experiment description of studies that identified B3GALT6 in exosomes
1
Experiment ID 235
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 237
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for B3GALT6
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which B3GALT6 is involved
PathwayEvidenceSource
A tetrasaccharide linker sequence is required for GAG synthesis TAS Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here