Gene description for LAS1L
Gene name LAS1-like (S. cerevisiae)
Gene symbol LAS1L
Other names/aliases Las1-like
dJ475B7.2
Species Homo sapiens
 Database cross references - LAS1L
ExoCarta ExoCarta_81887
Vesiclepedia VP_81887
Entrez Gene 81887
HGNC 25726
UniProt Q9Y4W2  
 LAS1L identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Thymus 23844026    
 Gene ontology annotations for LAS1L
Molecular Function
    RNA binding GO:0003723 HDA
    endonuclease activity GO:0004519 IEA
    protein binding GO:0005515 IPI
Biological Process
    maturation of 5.8S rRNA GO:0000460 IBA
    maturation of LSU-rRNA GO:0000470 IBA
    rRNA processing GO:0006364 IDA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleoplasm GO:0005654 TAS
    nucleolus GO:0005730 IDA
    membrane GO:0016020 HDA
    MLL1 complex GO:0071339 IDA
    Las1 complex GO:0090730 IEA
 Experiment description of studies that identified LAS1L in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for LAS1L
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 UBE2H 7328
Affinity Capture-MS Homo sapiens
3 SENP3 26168
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 SPRTN  
Affinity Capture-MS Homo sapiens
5 COIL  
Proximity Label-MS Homo sapiens
6 DNTTIP1 116092
Affinity Capture-MS Homo sapiens
7 HIST1H2BG 8339
Proximity Label-MS Homo sapiens
8 NOP56 10528
Proximity Label-MS Homo sapiens
9 RPL10 6134
Affinity Capture-MS Homo sapiens
10 C9orf78 51759
Affinity Capture-MS Homo sapiens
11 UBC 7316
Affinity Capture-MS Homo sapiens
12 CAND1 55832
Affinity Capture-MS Homo sapiens
13 SLC16A10 117247
Affinity Capture-MS Homo sapiens
14 CENPA  
Proximity Label-MS Homo sapiens
15 APEX1 328
Proximity Label-MS Homo sapiens
16 DDX23 9416
Proximity Label-MS Homo sapiens
17 NPM1 4869
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 MDC1  
Affinity Capture-MS Homo sapiens
19 FBL 2091
Proximity Label-MS Homo sapiens
20 POLR1E  
Proximity Label-MS Homo sapiens
21 OBSL1 23363
Affinity Capture-MS Homo sapiens
22 DDOST 1650
Affinity Capture-MS Homo sapiens
23 MAGED2 10916
Affinity Capture-MS Homo sapiens
24 BRD4 23476
Affinity Capture-MS Homo sapiens
25 SRP68 6730
Affinity Capture-MS Homo sapiens
26 TNFRSF13B  
Affinity Capture-MS Homo sapiens
27 DNAJC7 7266
Proximity Label-MS Homo sapiens
28 RBM42  
Affinity Capture-MS Homo sapiens
29 MAD2L2 10459
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 CTCF  
Affinity Capture-MS Homo sapiens
31 PSME3 10197
Affinity Capture-MS Homo sapiens
32 KLF16  
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 UFL1 23376
Affinity Capture-MS Homo sapiens
34 SRP9 6726
Affinity Capture-MS Homo sapiens
35 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
36 PARK2  
Affinity Capture-MS Homo sapiens
37 PNKP 11284
Affinity Capture-MS Homo sapiens
38 DRG1 4733
Affinity Capture-MS Homo sapiens
39 RPL5 6125
Affinity Capture-MS Homo sapiens
40 C12orf49  
Negative Genetic Homo sapiens
41 LHX1  
Proximity Label-MS Homo sapiens
42 PASK  
Affinity Capture-MS Homo sapiens
43 BARD1 580
Affinity Capture-MS Homo sapiens
44 NTRK1 4914
Affinity Capture-MS Homo sapiens
45 AIM2  
Affinity Capture-MS Homo sapiens
46 IFI16 3428
Affinity Capture-MS Homo sapiens
47 SUMO2 6613
Reconstituted Complex Homo sapiens
48 CBX6  
Affinity Capture-MS Homo sapiens
49 RPA4  
Proximity Label-MS Homo sapiens
50 RPN1 6184
Affinity Capture-MS Homo sapiens
51 PML 5371
Affinity Capture-MS Homo sapiens
52 HECTD1 25831
Affinity Capture-MS Homo sapiens
53 RPS16 6217
Affinity Capture-MS Homo sapiens
54 PELP1 27043
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
55 CD3EAP  
Proximity Label-MS Homo sapiens
56 ZBTB2 57621
Affinity Capture-MS Homo sapiens
57 TUBB 203068
Affinity Capture-MS Homo sapiens
58 IPO5 3843
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 RPN2 6185
Affinity Capture-MS Homo sapiens
60 SMARCAD1  
Affinity Capture-MS Homo sapiens
61 XPO1 7514
Affinity Capture-MS Homo sapiens
62 LMNB1 4001
Affinity Capture-MS Homo sapiens
63 ECT2 1894
Affinity Capture-MS Homo sapiens
64 SIRT7  
Affinity Capture-MS Homo sapiens
65 NPM3 10360
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 CUL3 8452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 RPS6 6194
Proximity Label-MS Homo sapiens
68 DUSP16  
Affinity Capture-MS Homo sapiens
69 LOC100132735  
Protein-RNA Homo sapiens
70 CEP250 11190
Affinity Capture-MS Homo sapiens
71 PSPC1 55269
Affinity Capture-MS Homo sapiens
72 RPA3 6119
Proximity Label-MS Homo sapiens
73 H2AFB1  
Affinity Capture-MS Homo sapiens
74 ETV3  
Proximity Label-MS Homo sapiens
75 RAF1 5894
Affinity Capture-MS Homo sapiens
76 DDRGK1 65992
Affinity Capture-MS Homo sapiens
77 RNF2  
Affinity Capture-MS Homo sapiens
78 BMI1  
Affinity Capture-MS Homo sapiens
79 HAX1  
Proximity Label-MS Homo sapiens
80 NEUROG3  
Affinity Capture-MS Homo sapiens
81 C9orf72  
Affinity Capture-MS Homo sapiens
82 PYHIN1  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
83 HUWE1 10075
Affinity Capture-MS Homo sapiens
84 OST4  
Affinity Capture-MS Homo sapiens
85 NES 10763
Affinity Capture-MS Homo sapiens
86 Cbx1  
Affinity Capture-MS Mus musculus
87 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
88 SIRT6  
Affinity Capture-MS Homo sapiens
89 PARP1 142
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
90 MNDA 4332
Affinity Capture-MS Homo sapiens
91 ZNF746  
Affinity Capture-MS Homo sapiens
92 USP36  
Affinity Capture-MS Homo sapiens
93 MEN1 4221
Affinity Capture-MS Homo sapiens
94 SRP19 6728
Affinity Capture-MS Homo sapiens
95 SRP72 6731
Affinity Capture-MS Homo sapiens
96 CAPRIN1 4076
Affinity Capture-MS Homo sapiens
97 NIFK 84365
Proximity Label-MS Homo sapiens
98 WDR18 57418
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
99 DHX40  
Proximity Label-MS Homo sapiens
100 RPS24 6229
Proximity Label-MS Homo sapiens
101 C16orf72 29035
Affinity Capture-MS Homo sapiens
102 RPL31 6160
Proximity Label-MS Homo sapiens
103 CIT 11113
Affinity Capture-MS Homo sapiens
104 STT3B 201595
Affinity Capture-MS Homo sapiens
105 CUL7 9820
Affinity Capture-MS Homo sapiens
106 GNB2L1 10399
Affinity Capture-MS Homo sapiens
107 KBTBD4  
Affinity Capture-MS Homo sapiens
108 TEX10 54881
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
109 NOL9 79707
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
110 CDC5L 988
Affinity Capture-MS Homo sapiens
111 RBM8A 9939
Affinity Capture-MS Homo sapiens
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