Gene description for PARG
Gene name poly (ADP-ribose) glycohydrolase
Gene symbol PARG
Other names/aliases PARG99
Species Homo sapiens
 Database cross references - PARG
ExoCarta ExoCarta_8505
Entrez Gene 8505
HGNC 8605
MIM 603501
UniProt Q86W56  
 PARG identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
 Gene ontology annotations for PARG
Molecular Function
    poly(ADP-ribose) glycohydrolase activity GO:0004649 IDA
Biological Process
    DNA repair GO:0006281 TAS
    base-excision repair GO:0006284 TAS
    carbohydrate metabolic process GO:0005975 IEA
    detection of bacterium GO:0016045 IEA
Subcellular Localization
    intracellular membrane-bounded organelle GO:0043231 IDA
    mitochondrial matrix GO:0005759 IEA
    cytosol GO:0005829 IEA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IDA
 Experiment description of studies that identified PARG in exosomes
1
Experiment ID 236
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for PARG
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CASP3 836
Biochemical Activity Homo sapiens
View the network image/svg+xml
 Pathways in which PARG is involved
PathwayEvidenceSource
POLB-Dependent Long Patch Base Excision Repair TAS Reactome





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