Gene description for LDHC
Gene name lactate dehydrogenase C
Gene symbol LDHC
Other names/aliases CT32
LDH3
LDHX
Species Homo sapiens
 Database cross references - LDHC
ExoCarta ExoCarta_3948
Vesiclepedia VP_3948
Entrez Gene 3948
HGNC 6544
MIM 150150
UniProt P07864  
 LDHC identified in exosomes derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for LDHC
Molecular Function
    L-lactate dehydrogenase activity GO:0004459 IBA
    protein binding GO:0005515 IPI
Biological Process
    lactate metabolic process GO:0006089 IBA
    lactate metabolic process GO:0006089 NAS
    pyruvate metabolic process GO:0006090 IBA
    pyruvate metabolic process GO:0006090 NAS
    ATP biosynthetic process GO:0006754 IEA
    lactate biosynthetic process from pyruvate GO:0019244 IEA
    flagellated sperm motility GO:0030317 IEA
    pyruvate catabolic process GO:0042867 IEA
Subcellular Localization
    nucleus GO:0005634 HDA
    mitochondrion GO:0005739 IBA
    cytosol GO:0005829 TAS
    motile cilium GO:0031514 IEA
    extracellular exosome GO:0070062 HDA
    oxidoreductase complex GO:1990204 NAS
 Experiment description of studies that identified LDHC in exosomes
1
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for LDHC
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LIG4 3981
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
2 NME2 4831
Co-fractionation Homo sapiens
3 LDHAL6A  
Affinity Capture-MS Homo sapiens
4 UBE2N 7334
Co-fractionation Homo sapiens
5 SOD1 6647
Co-fractionation Homo sapiens
6 ZNF675  
Affinity Capture-MS Homo sapiens
7 LDHB 3945
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 WDR1 9948
Co-fractionation Homo sapiens
9 NME1-NME2 654364
Co-fractionation Homo sapiens
10 LDHA 3939
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 TALDO1 6888
Co-fractionation Homo sapiens
12 ARF5 381
Co-fractionation Homo sapiens
13 RAB1B 81876
Co-fractionation Homo sapiens
14 BRCA1 672
Two-hybrid Homo sapiens
15 TLE3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 SSB 6741
Affinity Capture-MS Homo sapiens
17 ELMOD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 MED24 9862
Affinity Capture-MS Homo sapiens
19 RAB1A 5861
Co-fractionation Homo sapiens
20 ARF4 378
Co-fractionation Homo sapiens
21 LUCAT1  
Affinity Capture-RNA Homo sapiens
22 TM2D3  
Affinity Capture-MS Homo sapiens
23 TUBA1C 84790
Affinity Capture-MS Homo sapiens
24 FMO1  
Affinity Capture-MS Homo sapiens
25 RBL2 5934
Two-hybrid Homo sapiens
26 TPI1 7167
Co-fractionation Homo sapiens
27 ARF1 375
Co-fractionation Homo sapiens
28 SLC24A3  
Affinity Capture-MS Homo sapiens
29 SERBP1 26135
Affinity Capture-MS Homo sapiens
30 MDH1 4190
Co-fractionation Homo sapiens
31 DNAJC12  
Affinity Capture-MS Homo sapiens
32 NPAP1  
Affinity Capture-MS Homo sapiens
33 TSSC1 7260
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 COMT 1312
Affinity Capture-MS Homo sapiens
35 BRD4 23476
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which LDHC is involved
PathwayEvidenceSource
Aerobic respiration and respiratory electron transport TAS Reactome
Metabolism TAS Reactome
Pyruvate metabolism TAS Reactome





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