ExoCarta: Gene summary

Credits | Contact

Gene description for C1GALT1
Gene name	core 1 synthase, glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1
Gene symbol	C1GALT1
Other names/aliases	C1GALT T-synthase
Species	Homo sapiens
Database cross references - C1GALT1
ExoCarta	ExoCarta_56913
Vesiclepedia	VP_56913
Entrez Gene	56913
HGNC	24337
MIM	610555
UniProt	Q9NS00
C1GALT1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942
Hepatocytes	26054723

Gene ontology annotations for C1GALT1

Molecular Function
	nucleotide binding	GO:0000166	IEA
	protein binding	GO:0005515	IPI
	glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase activity	GO:0016263	IBA
	glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase activity	GO:0016263	IDA
	metal ion binding	GO:0046872	IEA
Biological Process
	angiogenesis	GO:0001525	IMP
	kidney development	GO:0001822	IMP
	protein O-linked glycosylation	GO:0006493	IEA
	intestinal epithelial cell development	GO:0060576	IEA
Subcellular Localization
	Golgi membrane	GO:0000139	TAS
	membrane	GO:0016020	IDA

Experiment description of studies that identified C1GALT1 in exosomes

Experiment ID

489

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 6

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

490

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 7

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

491

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 8

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

492

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 9

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

237

MISEV standards
✔ EM	Biophysical techniques
✔ TSG101\|Alix\|HSC70\|GAPDH	Enriched markers
✔ HSP90B1	Negative markers
✔ qNano	Particle analysis

Identified molecule

mRNA

Identification method

RNA Sequencing

PubMed ID

26054723

Organism

Homo sapiens

Experiment description

Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs

Authors

He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N

Journal name

Carcinogenesis

Publication year

2015

Sample

Hepatocytes

Sample name

MIHA

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient

Flotation density

1.13-1.19 g/mL

Molecules identified in the study

Protein
RNA

Methods used in the study

Western blotting
Mass spectrometry
RNA Sequencing

Protein-protein interactions for C1GALT1

Protein Interactor

ExoCarta ID

Identification method

PubMed

Species

CCL3

Affinity Capture-MS

Homo sapiens

C1GALT1C1

29071

Reconstituted Complex

Homo sapiens

TMEM52B

120939

Two-hybrid

Homo sapiens

LRRC15

131578

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

GJA8

Two-hybrid

Homo sapiens

GPX8

493869

Two-hybrid

Homo sapiens

XRN1

54464

Two-hybrid

Homo sapiens

GPR151

Two-hybrid

Homo sapiens

PIGP

Two-hybrid

Homo sapiens

HPN

Affinity Capture-MS

Homo sapiens

TMEM14B

Two-hybrid

Homo sapiens

CX3CL1

Affinity Capture-MS

Homo sapiens

GPR101

Two-hybrid

Homo sapiens

GOLPH3

64083

Affinity Capture-MS		Homo sapiens
Reconstituted Complex		Homo sapiens

DPEP1

1800

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

CD79A

Two-hybrid

Homo sapiens

GPRC5D

Two-hybrid

Homo sapiens

LEPROT

54741

Two-hybrid

Homo sapiens

ELOVL4

Two-hybrid

Homo sapiens

GOLPH3L

55204

Affinity Capture-MS		Homo sapiens
Reconstituted Complex		Homo sapiens

TMEM237

65062

Two-hybrid

Homo sapiens

LEUTX

Two-hybrid

Homo sapiens

CD27

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

FAM209A

200232

Two-hybrid

Homo sapiens

ANKS6

203286

Two-hybrid

Homo sapiens

CHRNB2

Affinity Capture-MS

Homo sapiens

GPR42

Two-hybrid

Homo sapiens

NTRK3

4916

Affinity Capture-MS

Homo sapiens

FAM134C

162427

Two-hybrid

Homo sapiens

STX1A

6804

Two-hybrid

Homo sapiens

CPLX4

Two-hybrid

Homo sapiens

CRP

Affinity Capture-MS

Homo sapiens

GJB1

2705

Two-hybrid

Homo sapiens

MUC1

4582

Two-hybrid

Homo sapiens

ST14

6768

Affinity Capture-MS

Homo sapiens

ARL13B

200894

Two-hybrid

Homo sapiens

SLC1A1

6505

Two-hybrid

Homo sapiens

LHFPL5

Two-hybrid

Homo sapiens

CSPG5

10675

Affinity Capture-MS

Homo sapiens

GJB5

Two-hybrid

Homo sapiens

TMPRSS13

Affinity Capture-MS

Homo sapiens

NPPB

Affinity Capture-MS

Homo sapiens

FAM210B

Two-hybrid

Homo sapiens

SLC14A1

Two-hybrid

Homo sapiens

View the network

Pathways in which C1GALT1 is involved

Pathway	Evidence	Source
Defective C1GALT1C1 causes TNPS	TAS	Reactome
Disease	TAS	Reactome
Diseases associated with O-glycosylation of proteins	TAS	Reactome
Diseases of glycosylation	TAS	Reactome
Diseases of metabolism	TAS	Reactome
Metabolism of proteins	TAS	Reactome
O-linked glycosylation	TAS	Reactome
O-linked glycosylation of mucins	TAS	Reactome
Post-translational protein modification	TAS	Reactome

Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here