Gene description for Nid1
Gene name nidogen 1
Gene symbol Nid1
Other names/aliases A630025O17
Nid
entactin
entactin-1
nidogen-1
Species Mus musculus
 Database cross references - Nid1
ExoCarta ExoCarta_18073
Vesiclepedia VP_18073
Entrez Gene 18073
UniProt P10493  
 Nid1 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Embryonic fibroblasts 18494037    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Nid1
Molecular Function
    proteoglycan binding GO:0043394 ISO
    extracellular matrix binding GO:0050840 IDA
    protein binding GO:0005515 IPI
    laminin-1 binding GO:0043237 IDA
    collagen binding GO:0005518 ISO
    laminin binding GO:0043236 ISO
    calcium ion binding GO:0005509 IEA
Biological Process
    positive regulation of cell-substrate adhesion GO:0010811 IDA
    extracellular matrix organization GO:0030198 IDA
    glomerular basement membrane development GO:0032836 IMP
    cell-matrix adhesion GO:0007160 IDA
    cell adhesion GO:0007155 IEA
Subcellular Localization
    extracellular exosome GO:0070062 ISO
    extracellular region GO:0005576 IEA
    basal lamina GO:0005605 IDA
    extracellular matrix GO:0031012 ISO
    cell periphery GO:0071944 IDA
    proteinaceous extracellular matrix GO:0005578 IDA
    basement membrane GO:0005604 TAS
 Experiment description of studies that identified Nid1 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 73
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Nid1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LAMC1 3915
Reconstituted Complex Homo sapiens
2 COL1A1 1277
Reconstituted Complex Homo sapiens
3 COL4A1 1282
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which Nid1 is involved
PathwayEvidenceSource
Degradation of the extracellular matrix IEA Reactome
Laminin interactions IEA Reactome





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