Gene description for PDGFA
Gene name platelet-derived growth factor alpha polypeptide
Gene symbol PDGFA
Other names/aliases PDGF-A
PDGF1
Species Homo sapiens
 Database cross references - PDGFA
ExoCarta ExoCarta_5154
Vesiclepedia VP_5154
Entrez Gene 5154
HGNC 8799
MIM 173430
UniProt P04085  
 PDGFA identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells Unpublished / Not applicable
 Gene ontology annotations for PDGFA
Molecular Function
    platelet-derived growth factor receptor binding GO:0005161 IBA
    platelet-derived growth factor receptor binding GO:0005161 IDA
    platelet-derived growth factor receptor binding GO:0005161 IPI
    protein binding GO:0005515 IPI
    collagen binding GO:0005518 IDA
    growth factor activity GO:0008083 IDA
    identical protein binding GO:0042802 IPI
    protein homodimerization activity GO:0042803 IDA
    protein heterodimerization activity GO:0046982 IPI
    platelet-derived growth factor binding GO:0048407 IPI
Biological Process
    angiogenesis GO:0001525 IBA
    angiogenesis GO:0001525 ISS
    cell activation GO:0001775 TAS
    hair follicle development GO:0001942 ISS
    positive regulation of mesenchymal cell proliferation GO:0002053 ISS
    cell-cell signaling GO:0007267 TAS
    cell population proliferation GO:0008283 IEA
    positive regulation of cell population proliferation GO:0008284 IBA
    positive regulation of cell population proliferation GO:0008284 IDA
    positive regulation of cell population proliferation GO:0008284 IDA
    response to wounding GO:0009611 IDA
    animal organ morphogenesis GO:0009887 ISS
    negative regulation of phosphatidylinositol biosynthetic process GO:0010512 IDA
    negative regulation of platelet activation GO:0010544 IDA
    regulation of smooth muscle cell migration GO:0014910 IDA
    cell projection assembly GO:0030031 ISS
    actin cytoskeleton organization GO:0030036 ISS
    positive regulation of cell migration GO:0030335 IBA
    positive regulation of cell migration GO:0030335 IDA
    positive regulation of protein autophosphorylation GO:0031954 IDA
    regulation of actin cytoskeleton organization GO:0032956 TAS
    platelet-derived growth factor receptor-alpha signaling pathway GO:0035790 NAS
    positive regulation of metanephric mesenchymal cell migration by platelet-derived growth factor receptor-beta signaling pathway GO:0035793 IDA
    wound healing GO:0042060 TAS
    positive regulation of MAP kinase activity GO:0043406 IDA
    positive regulation of MAPK cascade GO:0043410 IMP
    skin development GO:0043588 ISS
    platelet-derived growth factor receptor signaling pathway GO:0048008 IBA
    platelet-derived growth factor receptor signaling pathway GO:0048008 IDA
    platelet-derived growth factor receptor signaling pathway GO:0048008 NAS
    positive regulation of fibroblast proliferation GO:0048146 IDA
    lung alveolus development GO:0048286 ISS
    digestive tract development GO:0048565 IEA
    regulation of peptidyl-tyrosine phosphorylation GO:0050730 ISS
    negative chemotaxis GO:0050919 IDA
    positive regulation of cell division GO:0051781 IEA
    positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051897 IBA
    positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051897 IDA
    bone development GO:0060348 IEA
    regulation of branching involved in salivary gland morphogenesis by epithelial-mesenchymal signaling GO:0060683 ISS
    positive regulation of ERK1 and ERK2 cascade GO:0070374 IBA
    positive regulation of ERK1 and ERK2 cascade GO:0070374 IDA
    regulation of glomerular mesangial cell proliferation GO:0072124 IDA
    embryonic lung development GO:1990401 ISS
    regulation of DNA biosynthetic process GO:2000278 IDA
Subcellular Localization
    Golgi membrane GO:0000139 TAS
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IDA
    endoplasmic reticulum lumen GO:0005788 TAS
    Golgi lumen GO:0005796 TAS
    microvillus GO:0005902 ISS
    cell surface GO:0009986 IDA
    platelet alpha granule lumen GO:0031093 TAS
    platelet-derived growth factor complex GO:1990265 IPI
 Experiment description of studies that identified PDGFA in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Antibody array
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
 Protein-protein interactions for PDGFA
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 COL14A1 7373
Affinity Capture-MS Homo sapiens
2 ANAPC7 51434
Cross-Linking-MS (XL-MS) Homo sapiens
3 PPP1R9A  
Cross-Linking-MS (XL-MS) Homo sapiens
4 COL6A1 1291
Reconstituted Complex Homo sapiens
5 COL1A1 1277
Reconstituted Complex Homo sapiens
6 COL3A1 1281
Reconstituted Complex Homo sapiens
7 LAMB1 3912
Affinity Capture-MS Homo sapiens
8 COL1A2 1278
Reconstituted Complex Homo sapiens
9 PDGFRA 5156
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
10 SNX27 81609
Affinity Capture-MS Homo sapiens
11 COL5A1 1289
Reconstituted Complex Homo sapiens
12 MDFI  
Two-hybrid Homo sapiens
13 DDX58 23586
Affinity Capture-RNA Homo sapiens
14 COL4A1 1282
Reconstituted Complex Homo sapiens
15 PLCG1 5335
Affinity Capture-Western Homo sapiens
16 TXNDC16 57544
Affinity Capture-MS Homo sapiens
17 HSPG2 3339
Reconstituted Complex Homo sapiens
18 PDAP1 11333
Co-purification Homo sapiens
19 RDH8  
Affinity Capture-MS Homo sapiens
20 COL2A1 1280
Reconstituted Complex Homo sapiens
21 ASPH 444
Two-hybrid Homo sapiens
View the network image/svg+xml



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