Gene description for PDGFA
Gene name platelet-derived growth factor alpha polypeptide
Gene symbol PDGFA
Other names/aliases PDGF-A
PDGF1
Species Homo sapiens
 Database cross references - PDGFA
ExoCarta ExoCarta_5154
Vesiclepedia VP_5154
Entrez Gene 5154
HGNC 8799
MIM 173430
UniProt P04085  
 PDGFA identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells Unpublished / Not applicable
 Gene ontology annotations for PDGFA
Molecular Function
    protein binding GO:0005515 IPI
    platelet-derived growth factor receptor binding GO:0005161 IPI
    protein homodimerization activity GO:0042803 IDA
    platelet-derived growth factor binding GO:0048407 IPI
    growth factor activity GO:0008083 IDA
    protein heterodimerization activity GO:0046982 IPI
    collagen binding GO:0005518 IDA
Biological Process
    response to hypoxia GO:0001666 IEA
    positive regulation of mesenchymal cell proliferation GO:0002053 ISS
    positive regulation of protein kinase B signaling GO:0051897 IDA
    Fc-epsilon receptor signaling pathway GO:0038095 TAS
    cell activation GO:0001775 TAS
    response to wounding GO:0009611 IDA
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    regulation of DNA biosynthetic process GO:2000278 IDA
    regulation of peptidyl-tyrosine phosphorylation GO:0050730 ISS
    actin cytoskeleton organization GO:0030036 ISS
    positive regulation of DNA replication GO:0045740 IDA
    inner ear development GO:0048839 IEA
    fibroblast growth factor receptor signaling pathway GO:0008543 TAS
    negative regulation of phosphatidylinositol biosynthetic process GO:0010512 IDA
    regulation of glomerular mesangial cell proliferation GO:0072124 IDA
    positive regulation of cell division GO:0051781 IEA
    platelet-derived growth factor receptor signaling pathway GO:0048008 IDA
    negative regulation of platelet activation GO:0010544 IDA
    lung alveolus development GO:0048286 ISS
    embryonic lung development GO:1990401 ISS
    regulation of branching involved in salivary gland morphogenesis by epithelial-mesenchymal signaling GO:0060683 ISS
    phosphatidylinositol-mediated signaling GO:0048015 TAS
    response to drug GO:0042493 IEA
    blood coagulation GO:0007596 TAS
    transforming growth factor beta receptor signaling pathway GO:0007179 IEA
    response to retinoic acid GO:0032526 IEA
    positive regulation of ERK1 and ERK2 cascade GO:0070374 IDA
    cell-cell signaling GO:0007267 TAS
    skin development GO:0043588 ISS
    negative chemotaxis GO:0050919 IDA
    positive regulation of MAPK cascade GO:0043410 IMP
    angiogenesis GO:0001525 ISS
    innate immune response GO:0045087 TAS
    platelet activation GO:0030168 TAS
    platelet degranulation GO:0002576 TAS
    positive regulation of phosphatidylinositol 3-kinase signaling GO:0014068 IDA
    cell projection assembly GO:0030031 ISS
    regulation of smooth muscle cell migration GO:0014910 IDA
    extracellular matrix organization GO:0030198 TAS
    positive regulation of cell proliferation GO:0008284 IDA
    positive regulation of MAP kinase activity GO:0043406 IDA
    positive regulation of cell migration GO:0030335 IDA
    epidermal growth factor receptor signaling pathway GO:0007173 TAS
    positive regulation of fibroblast proliferation GO:0048146 IDA
    regulation of actin cytoskeleton organization GO:0032956 TAS
    positive regulation of metanephric mesenchymal cell migration by platelet-derived growth factor receptor-beta signaling pathway GO:0035793 IDA
    response to inorganic substance GO:0010035 IEA
    hair follicle development GO:0001942 ISS
    positive regulation of protein autophosphorylation GO:0031954 IDA
    response to estradiol GO:0032355 IEA
    organ morphogenesis GO:0009887 ISS
    wound healing GO:0042060 TAS
Subcellular Localization
    microvillus GO:0005902 ISS
    extracellular space GO:0005615 IDA
    cell surface GO:0009986 IDA
    Golgi membrane GO:0000139 TAS
    platelet alpha granule lumen GO:0031093 TAS
    endoplasmic reticulum lumen GO:0005788 TAS
    extracellular region GO:0005576 TAS
 Experiment description of studies that identified PDGFA in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 126
MISEV standards
✘ EV Biophysical techniques
✔
GAPDH
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Antibody array
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
 Protein-protein interactions for PDGFA
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HSPG2 3339
Invitro Homo sapiens
2 PLCG1 5335
Affinity Capture-Western Homo sapiens
3 PDAP1 11333
Invitro Homo sapiens
4 PDGFRA 5156
Reconstituted Complex Homo sapiens
5 SPARC 6678
Invitro Homo sapiens
View the network image/svg+xml
 Pathways in which PDGFA is involved
PathwayEvidenceSource
Non-integrin membrane-ECM interactions TAS Reactome
Signaling by PDGF TAS Reactome





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