Experiment description of studies that identified HIST1H4E in exosomes |
1 |
Experiment ID |
76 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
TSG101|GAPDH|HSP90
|
EV Cytosolic markers |
✔
CD81|CD9|CD63|LAMP1|MHCI
|
EV Membrane markers |
✔
HSP90B1
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
20224111
|
Organism |
Homo sapiens |
Experiment description |
Proteomics analysis of bladder cancer exosomes. |
Authors |
Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A. |
Journal name |
MCP
|
Publication year |
2010 |
Sample |
Bladder cancer cells |
Sample name |
HT1376 |
Isolation/purification methods |
Differential centrifugation Sucrose density gradient |
Flotation density |
1.10-1.19 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [MALDI TOF/TOF] Western blotting FACS |
|
|
2 |
Experiment ID |
20 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
HSP90
|
EV Cytosolic markers |
✔
CD63|CD81|LAMP1
|
EV Membrane markers |
✔
GOLGA2|cytochrome c
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
17956143
|
Organism |
Homo sapiens |
Experiment description |
Proteomic analysis of microvesicles derived from human colorectal cancer cells. |
Authors |
Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS |
Journal name |
JPR
|
Publication year |
2007 |
Sample |
Colorectal cancer cells |
Sample name |
HT29 |
Isolation/purification methods |
Differential centrifugation Sucrose density gradient Diafiltration |
Flotation density |
1.16 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [LTQ] Western blotting |
|
|
3 |
Experiment ID |
207 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
TSG101|HSP70
|
EV Cytosolic markers |
✔
FLOT1
|
EV Membrane markers |
✔
VDAC
|
EV Negative markers |
✔
NTA
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
23161513
|
Organism |
Homo sapiens |
Experiment description |
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. |
Authors |
Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ. |
Journal name |
Mol Cell Proteomics
|
Publication year |
2012 |
Sample |
Colorectal cancer cells |
Sample name |
DKO-1 |
Isolation/purification methods |
Differential centrifugation Filtration |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
4 |
Experiment ID |
208 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
TSG101|HSP70
|
EV Cytosolic markers |
✔
FLOT1
|
EV Membrane markers |
✔
VDAC
|
EV Negative markers |
✔
NTA
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
23161513
|
Organism |
Homo sapiens |
Experiment description |
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. |
Authors |
Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ. |
Journal name |
Mol Cell Proteomics
|
Publication year |
2012 |
Sample |
Colorectal cancer cells |
Sample name |
Dks-8 |
Isolation/purification methods |
Differential centrifugation Filtration |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
5 |
Experiment ID |
209 |
ISEV standards |
✘
|
EV Biophysical techniques |
✔
TSG101|HSP70
|
EV Cytosolic markers |
✔
FLOT1
|
EV Membrane markers |
✔
VDAC
|
EV Negative markers |
✔
NTA
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
23161513
|
Organism |
Homo sapiens |
Experiment description |
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. |
Authors |
Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ. |
Journal name |
Mol Cell Proteomics
|
Publication year |
2012 |
Sample |
Colorectal cancer cells |
Sample name |
DLD-1 |
Isolation/purification methods |
Differential centrifugation Filtration |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
6 |
Experiment ID |
282 |
ISEV standards |
✔
CEM
|
EV Biophysical techniques |
✔
Alix|TSG101
|
EV Cytosolic markers |
✔
CD63|CD81|EpCAM
|
EV Membrane markers |
✘
|
EV Negative markers |
✔
DLS
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25890246
|
Organism |
Homo sapiens |
Experiment description |
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. |
Authors |
Xu R, Greening DW, Rai A, Ji H, Simpson RJ. |
Journal name |
Methods
|
Publication year |
2015 |
Sample |
Colorectal cancer cells |
Sample name |
LIM1863 - Ultracentrifugation - Rep 1 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Centrifugal concentration |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry Western blotting |
|
|
7 |
Experiment ID |
283 |
ISEV standards |
✔
CEM
|
EV Biophysical techniques |
✔
Alix|TSG101
|
EV Cytosolic markers |
✔
CD63|CD81|EpCAM
|
EV Membrane markers |
✘
|
EV Negative markers |
✔
DLS
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25890246
|
Organism |
Homo sapiens |
Experiment description |
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. |
Authors |
Xu R, Greening DW, Rai A, Ji H, Simpson RJ. |
Journal name |
Methods
|
Publication year |
2015 |
Sample |
Colorectal cancer cells |
Sample name |
LIM1863 - Ultracentrifugation - Rep 2 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Centrifugal concentration |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry Western blotting |
|
|
8 |
Experiment ID |
285 |
ISEV standards |
✔
CEM
|
EV Biophysical techniques |
✔
Alix|TSG101
|
EV Cytosolic markers |
✔
CD63|CD81|EpCAM
|
EV Membrane markers |
✘
|
EV Negative markers |
✔
DLS
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25890246
|
Organism |
Homo sapiens |
Experiment description |
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. |
Authors |
Xu R, Greening DW, Rai A, Ji H, Simpson RJ. |
Journal name |
Methods
|
Publication year |
2015 |
Sample |
Colorectal cancer cells |
Sample name |
LIM1863 - Sequential centrifugal ultrafiltration - Rep 1 |
Isolation/purification methods |
Differential centrifugation Filtration Sequential centrifugal ultrafiltration Centrifugal concentration |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry Western blotting |
|
|
9 |
Experiment ID |
286 |
ISEV standards |
✔
CEM
|
EV Biophysical techniques |
✔
Alix|TSG101
|
EV Cytosolic markers |
✔
CD63|CD81|EpCAM
|
EV Membrane markers |
✘
|
EV Negative markers |
✔
DLS
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25890246
|
Organism |
Homo sapiens |
Experiment description |
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. |
Authors |
Xu R, Greening DW, Rai A, Ji H, Simpson RJ. |
Journal name |
Methods
|
Publication year |
2015 |
Sample |
Colorectal cancer cells |
Sample name |
LIM1863 - Sequential centrifugal ultrafiltration - Rep 2 |
Isolation/purification methods |
Differential centrifugation Filtration Sequential centrifugal ultrafiltration Centrifugal concentration |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry Western blotting |
|
|
10 |
Experiment ID |
226 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
GAPDH
|
EV Cytosolic markers |
✔
CD9|FLOT1
|
EV Membrane markers |
✔
LMNA|H2AFX|ATP5A1|TOMM20
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
mRNA
|
Identification method |
Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
|
PubMed ID |
26027894
|
Organism |
Homo sapiens |
Experiment description |
Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting |
Authors |
Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar |
Journal name |
Journal of Extracellular Vesicles
|
Publication year |
2015 |
Sample |
Endothelial cells |
Sample name |
HMEC-1 |
Isolation/purification methods |
Differential ultracentrifugation Sucrose density gradient |
Flotation density |
1.10 g/mL
|
Molecules identified in the study |
miRNA Protein snoRNA lncRNA yRNA snRNA mRNA ncRNA mtRNA vtRNA scaRNA lincRNA |
Methods used in the study |
Small RNA sequencing (Illumina HiSeq 2000 (Solexa) Western blotting |
|
|
11 |
Experiment ID |
189 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✘
|
EV Cytosolic markers |
✘
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
19530224
|
Organism |
Homo sapiens |
Experiment description |
Profile of exosomes related proteins released by differentiated and undifferentiated human keratinocytes. |
Authors |
Chavez-Muñoz C, Kilani RT, Ghahary A. |
Journal name |
J Cell Physiol
|
Publication year |
2009 |
Sample |
Keratinocytes |
Sample name |
Keratinocytes - Differentiated |
Isolation/purification methods |
Differential centrifugation Filtration Sucrose cushion Filtration |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
12 |
Experiment ID |
190 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
HSC70
|
EV Cytosolic markers |
✘
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
19530224
|
Organism |
Homo sapiens |
Experiment description |
Profile of exosomes related proteins released by differentiated and undifferentiated human keratinocytes. |
Authors |
Chavez-Muñoz C, Kilani RT, Ghahary A. |
Journal name |
J Cell Physiol
|
Publication year |
2009 |
Sample |
Keratinocytes |
Sample name |
Keratinocytes - Undifferentiated |
Isolation/purification methods |
Differential centrifugation Filtration Sucrose cushion Filtration |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
13 |
Experiment ID |
126 |
ISEV standards |
✘
|
EV Biophysical techniques |
✔
GAPDH
|
EV Cytosolic markers |
✘
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry [LTQ-FT Ultra]
|
PubMed ID |
Unpublished / Not applicable
|
Organism |
Homo sapiens |
Experiment description |
Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy? |
Authors |
Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim |
Journal name |
Mesenchymal Stem Cell Therapy
|
Publication year |
2011 |
Sample |
Mesenchymal stem cells |
Sample name |
huES9.E1 |
Isolation/purification methods |
HPLC |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Antobody array Mass spectrometry |
|
|
14 |
Experiment ID |
224 |
ISEV standards |
✔
EM|AFM
|
EV Biophysical techniques |
✔
Alix|TSG101
|
EV Cytosolic markers |
✔
CD63|CD81
|
EV Membrane markers |
✔
GOLGA2
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25944692
|
Organism |
Homo sapiens |
Experiment description |
Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes |
Authors |
Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S |
Journal name |
Oncotarget
|
Publication year |
2015 |
Sample |
Neuroblastoma cells |
Sample name |
SH-SY5Y |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation OptiPrep density gradient |
Flotation density |
1.10 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry Western blotting |
|
|
15 |
Experiment ID |
211 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
TSG101|Alix
|
EV Cytosolic markers |
✔
EpCAM|TFRC
|
EV Membrane markers |
✔
cytochrome c|GOLGA2
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
23333927
|
Organism |
Homo sapiens |
Experiment description |
Characterization and proteomic analysis of ovarian cancer-derived exosomes. |
Authors |
Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K. |
Journal name |
J Proteomics
|
Publication year |
2013 |
Sample |
Ovarian cancer cells |
Sample name |
IGROV1 |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation Sucrose density gradient |
Flotation density |
1.09-1.15 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
16 |
Experiment ID |
212 |
ISEV standards |
✔
CEM
|
EV Biophysical techniques |
✔
TSG101|Alix
|
EV Cytosolic markers |
✔
EpCAM|TFRC
|
EV Membrane markers |
✔
Cytochrome C|GOLGA2
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
23333927
|
Organism |
Homo sapiens |
Experiment description |
Characterization and proteomic analysis of ovarian cancer-derived exosomes. |
Authors |
Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K. |
Journal name |
J Proteomics
|
Publication year |
2013 |
Sample |
Ovarian cancer cells |
Sample name |
OVCAR-3 |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation Sucrose density gradient |
Flotation density |
1.09-1.15 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
17 |
Experiment ID |
138 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
HSP70|HSP90|RAB5
|
EV Cytosolic markers |
✔
LAMP2|CD9
|
EV Membrane markers |
✔
HSP90B1
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
|
PubMed ID |
22723089
|
Organism |
Homo sapiens |
Experiment description |
Prostate cancer cell derived exosomes |
Authors |
Hosseini-Beheshti E, Guns ES. |
Journal name |
MCP
|
Publication year |
2012 |
Sample |
Prostate cancer cells |
Sample name |
DU145 - Rep 2 |
Isolation/purification methods |
Sucrose density gradient |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [QTOF] |
|
|
18 |
Experiment ID |
139 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
HSP70|HSP90|RAB5
|
EV Cytosolic markers |
✔
LAMP2|CD9
|
EV Membrane markers |
✔
HSP90B1
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
|
PubMed ID |
22723089
|
Organism |
Homo sapiens |
Experiment description |
Prostate cancer cell derived exosomes |
Authors |
Hosseini-Beheshti E, Guns ES. |
Journal name |
MCP
|
Publication year |
2012 |
Sample |
Prostate cancer cells |
Sample name |
DU145 - Rep 3 |
Isolation/purification methods |
Sucrose density gradient |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [QTOF] |
|
|
19 |
Experiment ID |
140 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
HSP70|HSP90|RAB5
|
EV Cytosolic markers |
✔
LAMP2|CD9
|
EV Membrane markers |
✔
HSP90B1
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
|
PubMed ID |
22723089
|
Organism |
Homo sapiens |
Experiment description |
Prostate cancer cell derived exosomes |
Authors |
Hosseini-Beheshti E, Guns ES. |
Journal name |
MCP
|
Publication year |
2012 |
Sample |
Prostate cancer cells |
Sample name |
VCaP - Rep 2 |
Isolation/purification methods |
Sucrose density gradient |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [QTOF] |
|
|
20 |
Experiment ID |
141 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
HSP70|HSP90|RAB5
|
EV Cytosolic markers |
✔
LAMP2|CD9
|
EV Membrane markers |
✔
HSP90B1
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
|
PubMed ID |
22723089
|
Organism |
Homo sapiens |
Experiment description |
Prostate cancer cell derived exosomes |
Authors |
Hosseini-Beheshti E, Guns ES. |
Journal name |
MCP
|
Publication year |
2012 |
Sample |
Prostate cancer cells |
Sample name |
VCaP - Rep 3 |
Isolation/purification methods |
Sucrose density gradient |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [QTOF] |
|
|
21 |
Experiment ID |
142 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
HSP70|HSP90|RAB5
|
EV Cytosolic markers |
✔
LAMP2|CD9
|
EV Membrane markers |
✔
HSP90B1
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
|
PubMed ID |
22723089
|
Organism |
Homo sapiens |
Experiment description |
Prostate cancer cell derived exosomes |
Authors |
Hosseini-Beheshti E, Guns ES. |
Journal name |
MCP
|
Publication year |
2012 |
Sample |
Prostate cancer cells |
Sample name |
LNCaP - Rep 2 |
Isolation/purification methods |
Sucrose density gradient |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [QTOF] |
|
|
22 |
Experiment ID |
143 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
HSP70|HSP90|RAB5
|
EV Cytosolic markers |
✔
LAMP2|CD9
|
EV Membrane markers |
✔
HSP90B1
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
|
PubMed ID |
22723089
|
Organism |
Homo sapiens |
Experiment description |
Prostate cancer cell derived exosomes |
Authors |
Hosseini-Beheshti E, Guns ES. |
Journal name |
MCP
|
Publication year |
2012 |
Sample |
Prostate cancer cells |
Sample name |
LNCaP - Rep 3 |
Isolation/purification methods |
Sucrose density gradient |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [QTOF] |
|
|
23 |
Experiment ID |
144 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
HSP70|HSP90|RAB5
|
EV Cytosolic markers |
✔
CD9
|
EV Membrane markers |
✔
HSP90B1
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
|
PubMed ID |
22723089
|
Organism |
Homo sapiens |
Experiment description |
Prostate cancer cell derived exosomes |
Authors |
Hosseini-Beheshti E, Guns ES. |
Journal name |
MCP
|
Publication year |
2012 |
Sample |
Prostate cancer cells |
Sample name |
C4-2 - Rep 2 |
Isolation/purification methods |
Sucrose density gradient |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [QTOF] |
|
|
24 |
Experiment ID |
145 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
HSP70|HSP90|RAB5
|
EV Cytosolic markers |
✔
CD10
|
EV Membrane markers |
✔
HSP90B1
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
|
PubMed ID |
22723089
|
Organism |
Homo sapiens |
Experiment description |
Prostate cancer cell derived exosomes |
Authors |
Hosseini-Beheshti E, Guns ES. |
Journal name |
MCP
|
Publication year |
2012 |
Sample |
Prostate cancer cells |
Sample name |
C4-2 - Rep 3 |
Isolation/purification methods |
Sucrose density gradient |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [QTOF] |
|
|
25 |
Experiment ID |
146 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
HSP70|HSP90
|
EV Cytosolic markers |
✔
CD9
|
EV Membrane markers |
✔
HSP90B1
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
|
PubMed ID |
22723089
|
Organism |
Homo sapiens |
Experiment description |
Prostate cancer cell derived exosomes |
Authors |
Hosseini-Beheshti E, Guns ES. |
Journal name |
MCP
|
Publication year |
2012 |
Sample |
Prostate cancer cells |
Sample name |
RWPE - Rep 2 |
Isolation/purification methods |
Sucrose density gradient |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [QTOF] |
|
|
26 |
Experiment ID |
66 |
ISEV standards |
✔
IEM
|
EV Biophysical techniques |
✔
TSG101|Alix
|
EV Cytosolic markers |
✔
CD63|CD81
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
19199708
|
Organism |
Homo sapiens |
Experiment description |
Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT). |
Authors |
Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR |
Journal name |
JPR
|
Publication year |
2009 |
Sample |
Saliva |
Sample name |
Saliva |
Isolation/purification methods |
Differential centrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [LTQ] Western blotting Immunoelectron Microscopy |
|
|
27 |
Experiment ID |
191 |
ISEV standards |
✘
|
EV Biophysical techniques |
✔
Alix
|
EV Cytosolic markers |
✔
CD81|CD9
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
20124223
|
Organism |
Homo sapiens |
Experiment description |
Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes. |
Authors |
Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK. |
Journal name |
Mol Cell Proteomics
|
Publication year |
2010 |
Sample |
Squamous carcinoma cells |
Sample name |
Squamous carcinoma cell (A431) |
Isolation/purification methods |
Differential centrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
28 |
Experiment ID |
217 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
TSG101
|
EV Cytosolic markers |
✔
CD81|CD9|CD63
|
EV Membrane markers |
✘
|
EV Negative markers |
✔
NTA
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
23844026
|
Organism |
Homo sapiens |
Experiment description |
Characterization of human thymic exosomes. |
Authors |
Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O. |
Journal name |
PLoS One
|
Publication year |
2013 |
Sample |
Thymus |
Sample name |
Normal-Thymus |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
29 |
Experiment ID |
196 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
Alix|TSG101|HSP70
|
EV Cytosolic markers |
✔
CD9
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
22418980
|
Organism |
Homo sapiens |
Experiment description |
A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes. |
Authors |
Raj DA, Fiume I, Capasso G, Pocsfalvi G. |
Journal name |
Kidney Int
|
Publication year |
2012 |
Sample |
Urine |
Sample name |
Urine - Normal high density |
Isolation/purification methods |
Differential centrifugation Sucrose cushion |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
30 |
Experiment ID |
197 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
Alix|TSG101|HSP70
|
EV Cytosolic markers |
✔
CD9
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
22418980
|
Organism |
Homo sapiens |
Experiment description |
A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes. |
Authors |
Raj DA, Fiume I, Capasso G, Pocsfalvi G. |
Journal name |
Kidney Int
|
Publication year |
2012 |
Sample |
Urine |
Sample name |
Urine - Normal low density |
Isolation/purification methods |
Differential centrifugation Sucrose cushion |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|