Gene description for SLC1A3
Gene name solute carrier family 1 (glial high affinity glutamate transporter), member 3
Gene symbol SLC1A3
Other names/aliases EA6
EAAT1
GLAST
GLAST1
Species Homo sapiens
 Database cross references - SLC1A3
ExoCarta ExoCarta_6507
Vesiclepedia VP_6507
Entrez Gene 6507
HGNC 10941
MIM 600111
UniProt P43003  
 SLC1A3 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for SLC1A3
Molecular Function
    L-glutamate transmembrane transporter activity GO:0005313 IBA
    L-glutamate transmembrane transporter activity GO:0005313 IDA
    high-affinity L-glutamate transmembrane transporter activity GO:0005314 IDA
    high-affinity L-glutamate transmembrane transporter activity GO:0005314 IMP
    high-affinity L-glutamate transmembrane transporter activity GO:0005314 TAS
    protein binding GO:0005515 IPI
    neutral L-amino acid transmembrane transporter activity GO:0015175 IBA
    glutamate:sodium symporter activity GO:0015501 IBA
    glutamate:sodium symporter activity GO:0015501 IDA
    glutamate:sodium symporter activity GO:0015501 IMP
    glutamate binding GO:0016595 IEA
    metal ion binding GO:0046872 IEA
Biological Process
    neurotransmitter uptake GO:0001504 TAS
    monoatomic ion transport GO:0006811 TAS
    neurotransmitter transport GO:0006836 TAS
    intracellular sodium ion homeostasis GO:0006883 IEA
    chemical synaptic transmission GO:0007268 TAS
    sensory perception of sound GO:0007605 IEA
    response to xenobiotic stimulus GO:0009410 IEA
    response to light stimulus GO:0009416 IEA
    gamma-aminobutyric acid biosynthetic process GO:0009449 IEA
    response to wounding GO:0009611 IEA
    neutral amino acid transport GO:0015804 IEA
    L-glutamate transmembrane transport GO:0015813 IDA
    cranial nerve development GO:0021545 IEA
    auditory behavior GO:0031223 IEA
    response to antibiotic GO:0046677 IEA
    cell morphogenesis involved in neuron differentiation GO:0048667 IEA
    positive regulation of synaptic transmission GO:0050806 IEA
    neuromuscular process controlling balance GO:0050885 IEA
    L-glutamate import GO:0051938 IDA
    transepithelial transport GO:0070633 ISS
    D-aspartate import across plasma membrane GO:0070779 IBA
    D-aspartate import across plasma membrane GO:0070779 IDA
    D-aspartate import across plasma membrane GO:0070779 IMP
    cellular response to cocaine GO:0071314 IEA
    potassium ion transmembrane transport GO:0071805 IDA
    L-glutamate import across plasma membrane GO:0098712 IBA
    L-glutamate import across plasma membrane GO:0098712 IDA
    L-glutamate import across plasma membrane GO:0098712 IMP
    L-aspartate import across plasma membrane GO:0140009 IBA
    L-aspartate import across plasma membrane GO:0140009 IDA
    L-aspartate import across plasma membrane GO:0140009 IMP
    transport across blood-brain barrier GO:0150104 ISS
    transport across blood-brain barrier GO:0150104 NAS
    chloride transmembrane transport GO:1902476 IDA
Subcellular Localization
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IMP
    plasma membrane GO:0005886 ISS
    plasma membrane GO:0005886 TAS
    basal plasma membrane GO:0009925 ISS
    cell surface GO:0009986 IEA
    membrane GO:0016020 TAS
    cytoplasmic vesicle GO:0031410 ISS
    neuron projection GO:0043005 ISS
    neuronal cell body GO:0043025 ISS
    synapse GO:0045202 IEA
    perinuclear region of cytoplasm GO:0048471 ISS
    membrane protein complex GO:0098796 ISS
 Experiment description of studies that identified SLC1A3 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
12
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SLC1A3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RAMP3  
Affinity Capture-MS Homo sapiens
2 LGALS9 3965
Affinity Capture-MS Homo sapiens
3 RAB35 11021
Proximity Label-MS Homo sapiens
4 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
5 KIF14 9928
Affinity Capture-MS Homo sapiens
6 VAPB 9217
Affinity Capture-MS Homo sapiens
7 LAMTOR1 55004
Proximity Label-MS Homo sapiens
8 MARCKS 4082
Proximity Label-MS Homo sapiens
9 PRNP 5621
Affinity Capture-MS Homo sapiens
10 RAB5A 5868
Proximity Label-MS Homo sapiens
11 LAMP3  
Proximity Label-MS Homo sapiens
12 HLA-B 3106
Affinity Capture-MS Homo sapiens
13 ZDHHC17 23390
Two-hybrid Homo sapiens
14 LAMP1 3916
Proximity Label-MS Homo sapiens
15 CLEC2D  
Affinity Capture-MS Homo sapiens
16 LGALS3 3958
Affinity Capture-MS Homo sapiens
17 MGARP  
Affinity Capture-MS Homo sapiens
18 CUL3 8452
Affinity Capture-MS Homo sapiens
19 NR3C1 2908
Proximity Label-MS Homo sapiens
20 ATG9A 79065
Proximity Label-MS Homo sapiens
21 ARF6 382
Proximity Label-MS Homo sapiens
22 CLSTN1 22883
Affinity Capture-MS Homo sapiens
23 SNX27 81609
Affinity Capture-MS Homo sapiens
24 LYN 4067
Proximity Label-MS Homo sapiens
25 SNX9 51429
Affinity Capture-MS Homo sapiens
26 CYFIP1 23191
Affinity Capture-MS Homo sapiens
27 VSIG4  
Affinity Capture-MS Homo sapiens
28 DIABLO 56616
Affinity Capture-MS Homo sapiens
29 PLD3 23646
Affinity Capture-MS Homo sapiens
30 RAB2A 5862
Proximity Label-MS Homo sapiens
31 PMAIP1  
Affinity Capture-MS Homo sapiens
32 RAB11A 8766
Proximity Label-MS Homo sapiens
33 OBSCN 84033
Affinity Capture-MS Homo sapiens
34 KCTD17 79734
Affinity Capture-MS Homo sapiens
35 MB21D1  
Proximity Label-MS Homo sapiens
36 DHFRL1  
Affinity Capture-MS Homo sapiens
37 KRAS 3845
Proximity Label-MS Homo sapiens
38 Kctd5  
Affinity Capture-MS Mus musculus
39 ABI1 10006
Affinity Capture-MS Homo sapiens
40 PDZK1 5174
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 SLC1A1 6505
Affinity Capture-MS Homo sapiens
42 CAV1 857
Proximity Label-MS Homo sapiens
43 RHOB 388
Proximity Label-MS Homo sapiens
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