Gene description for SLC1A3
Gene name solute carrier family 1 (glial high affinity glutamate transporter), member 3
Gene symbol SLC1A3
Other names/aliases EA6
EAAT1
GLAST
GLAST1
Species Homo sapiens
 Database cross references - SLC1A3
ExoCarta ExoCarta_6507
Entrez Gene 6507
HGNC 10941
MIM 600111
UniProt P43003  
 SLC1A3 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for SLC1A3
Molecular Function
    sodium:dicarboxylate symporter activity GO:0017153 IEA
    L-glutamate transmembrane transporter activity GO:0005313 IDA
    high-affinity glutamate transmembrane transporter activity GO:0005314 IEA
    glutamate binding GO:0016595 IEA
Biological Process
    neuromuscular process controlling balance GO:0050885 IEA
    D-aspartate import GO:0070779 IDA
    positive regulation of synaptic transmission GO:0050806 IEA
    L-glutamate transmembrane transport GO:0089711 IEA
    positive regulation of defense response to virus by host GO:0002230 IMP
    ion transport GO:0006811 TAS
    response to light stimulus GO:0009416 IEA
    cell morphogenesis involved in neuron differentiation GO:0048667 IEA
    response to antibiotic GO:0046677 IEA
    response to drug GO:0042493 IEA
    glutamate biosynthetic process GO:0006537 IEA
    sensory perception of sound GO:0007605 IEA
    glutamate secretion GO:0014047 TAS
    activation of mitophagy in response to mitochondrial depolarization GO:0098779 IMP
    neurotransmitter secretion GO:0007269 TAS
    response to wounding GO:0009611 IEA
    neurotransmitter uptake GO:0001504 TAS
    synaptic transmission GO:0007268 TAS
    auditory behavior GO:0031223 IEA
    transmembrane transport GO:0055085 TAS
    L-glutamate import GO:0051938 IDA
    cranial nerve development GO:0021545 IEA
    gamma-aminobutyric acid biosynthetic process GO:0009449 IEA
Subcellular Localization
    cell projection GO:0042995 IEA
    integral component of membrane GO:0016021 IEA
    membrane GO:0016020 TAS
    cell surface GO:0009986 IEA
    fibril GO:0043205 IEA
    neuronal cell body GO:0043025 IEA
    plasma membrane GO:0005886 TAS
 Experiment description of studies that identified SLC1A3 in exosomes
1
Experiment ID 207
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
✘ EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SLC1A3
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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