Gene description for PFKFB4
Gene name 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4
Gene symbol PFKFB4
Other names/aliases -
Species Homo sapiens
 Database cross references - PFKFB4
ExoCarta ExoCarta_5210
Vesiclepedia VP_5210
Entrez Gene 5210
HGNC 8875
MIM 605320
UniProt Q16877  
 PFKFB4 identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
 Gene ontology annotations for PFKFB4
Molecular Function
    6-phosphofructo-2-kinase activity GO:0003873 IBA
    6-phosphofructo-2-kinase activity GO:0003873 NAS
    fructose-2,6-bisphosphate 2-phosphatase activity GO:0004331 IBA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
Biological Process
    fructose metabolic process GO:0006000 IEA
    fructose 2,6-bisphosphate metabolic process GO:0006003 IBA
    carbohydrate phosphorylation GO:0046835 IEA
Subcellular Localization
    cytosol GO:0005829 IBA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified PFKFB4 in exosomes
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for PFKFB4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YWHAH 7533
Affinity Capture-MS Homo sapiens
2 YWHAE 7531
Affinity Capture-MS Homo sapiens
3 GADD45A  
Two-hybrid Homo sapiens
4 NXF1 10482
Affinity Capture-RNA Homo sapiens
5 YWHAQ 10971
Affinity Capture-MS Homo sapiens
6 PFKFB2 5208
Affinity Capture-MS Homo sapiens
7 PFKFB1  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
8 PFKFB3 5209
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 GNG12 55970
Affinity Capture-MS Homo sapiens
10 YWHAG 7532
Affinity Capture-MS Homo sapiens
11 OBSL1 23363
Affinity Capture-MS Homo sapiens
12 YWHAZ 7534
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 CSNK2B 1460
Two-hybrid Homo sapiens
14 FKBP9P1 360132
Affinity Capture-MS Homo sapiens
15 RRAS 6237
Affinity Capture-MS Homo sapiens
16 LRR1  
Two-hybrid Homo sapiens
17 GSK3B 2932
Two-hybrid Homo sapiens
18 CCDC102B  
Two-hybrid Homo sapiens
19 YWHAB 7529
Affinity Capture-MS Homo sapiens
20 SLC2A8 29988
Affinity Capture-MS Homo sapiens
21 FBXL7  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
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