Gene description for DACT1
Gene name dishevelled-binding antagonist of beta-catenin 1
Gene symbol DACT1
Other names/aliases DAPPER
DAPPER1
DPR1
FRODO
HDPR1
THYEX3
Species Homo sapiens
 Database cross references - DACT1
ExoCarta ExoCarta_51339
Entrez Gene 51339
HGNC 17748
MIM 607861
UniProt Q9NYF0  
 DACT1 identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
 Gene ontology annotations for DACT1
Molecular Function
    protein kinase C binding GO:0005080 ISS
    protein binding GO:0005515 IPI
    beta-catenin binding GO:0008013 IDA
    delta-catenin binding GO:0070097 ISS
    protein kinase A binding GO:0051018 IDA
Biological Process
    positive regulation of catenin import into nucleus GO:0035413 IMP
    negative regulation of canonical Wnt signaling pathway GO:0090090 TAS
    regulation of protein stability GO:0031647 IDA
    regulation of nodal signaling pathway GO:1900107 IDA
    Wnt signaling pathway GO:0016055 IEA
    synapse organization GO:0050808 IEA
    gastrulation with mouth forming second GO:0001702 IEA
    positive regulation of fat cell differentiation GO:0045600 IEA
    positive regulation of protein catabolic process GO:0045732 IDA
    negative regulation of Wnt signaling pathway GO:0030178 IMP
    regulation of catenin import into nucleus GO:0035412 IMP
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 IDA
    embryonic hindgut morphogenesis GO:0048619 ISS
    negative regulation of G1/S transition of mitotic cell cycle GO:2000134 IDA
    dendrite morphogenesis GO:0048813 IEA
    regulation of Wnt signaling pathway, planar cell polarity pathway GO:2000095 ISS
    negative regulation of JNK cascade GO:0046329 IDA
    positive regulation of Wnt signaling pathway GO:0030177 IDA
Subcellular Localization
    cell junction GO:0030054 IEA
    cytoplasm GO:0005737 IDA
    beta-catenin destruction complex GO:0030877 IDA
    cytosol GO:0005829 TAS
    nucleoplasm GO:0005654 IDA
    synapse GO:0045202 IEA
 Experiment description of studies that identified DACT1 in exosomes
1
Experiment ID 224
ISEV standards
EM|AFM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81
EV Membrane markers
GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for DACT1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which DACT1 is involved
PathwayEvidenceSource
degradation of DVL TAS Reactome





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