Gene description for SBF1
Gene name SET binding factor 1
Gene symbol SBF1
Other names/aliases CMT4B3
DENND7A
MTMR5
Species Homo sapiens
 Database cross references - SBF1
ExoCarta ExoCarta_6305
Entrez Gene 6305
HGNC 10542
MIM 603560
UniProt O95248  
 SBF1 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Thymus 23844026    
 Gene ontology annotations for SBF1
Molecular Function
    Rab guanyl-nucleotide exchange factor activity GO:0017112 IDA
    phosphatase regulator activity GO:0019208 IEA
    protein tyrosine/serine/threonine phosphatase activity GO:0008138 TAS
Biological Process
    positive regulation of GTPase activity GO:0043547 IEA
    protein dephosphorylation GO:0006470 TAS
    spermatogenesis GO:0007283 IEA
Subcellular Localization
    integral component of membrane GO:0016021 TAS
    nucleus GO:0005634 IEA
 Experiment description of studies that identified SBF1 in exosomes
1
Experiment ID 235
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SBF1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MTMR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
2 SUV39H1  
Co-localization Homo sapiens
Synthetic Rescue Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which SBF1 is involved
No pathways found





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