Gene description for IFT27
Gene name intraflagellar transport 27
Gene symbol IFT27
Other names/aliases BBS19
RABL4
RAYL
Species Homo sapiens
 Database cross references - IFT27
ExoCarta ExoCarta_11020
Entrez Gene 11020
HGNC 18626
MIM 615870
UniProt Q9BW83  
 IFT27 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for IFT27
Molecular Function
    GTPase activity GO:0003924 IBA
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IEA
Biological Process
    organelle organization GO:0006996 TAS
    intracellular protein transport GO:0006886 ISS
    intraciliary transport GO:0042073 ISS
    smoothened signaling pathway GO:0007224 ISS
    Rab protein signal transduction GO:0032482 IBA
    metabolic process GO:0008152 IBA
Subcellular Localization
    sperm midpiece GO:0097225 IEA
    cilium GO:0005929 IDA
    centrosome GO:0005813 IEA
    ciliary tip GO:0097542 TAS
    motile cilium GO:0031514 ISS
    sperm principal piece GO:0097228 IEA
    intraciliary transport particle B GO:0030992 ISS
    primary cilium GO:0072372 TAS
 Experiment description of studies that identified IFT27 in exosomes
1
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for IFT27
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 C1orf41  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which IFT27 is involved
PathwayEvidenceSource
Intraflagellar transport TAS Reactome





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