Experiment ID |
20 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
HSP90
|
EV Cytosolic markers |
✔
CD63|CD81|LAMP1
|
EV Membrane markers |
✔
GOLGA2|cytochrome c
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
17956143
|
Organism |
Homo sapiens |
Experiment description |
Proteomic analysis of microvesicles derived from human colorectal cancer cells. |
Authors |
Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS |
Journal name |
JPR
|
Publication year |
2007 |
Sample |
Colorectal cancer cells |
Sample name |
HT29 |
Isolation/purification methods |
Differential centrifugation Sucrose density gradient Diafiltration |
Flotation density |
1.16 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [LTQ] Western blotting |