Gene description for MAN1B1
Gene name mannosidase, alpha, class 1B, member 1
Gene symbol MAN1B1
Other names/aliases ERMAN1
MANA-ER
MRT15
Species Homo sapiens
 Database cross references - MAN1B1
ExoCarta ExoCarta_11253
Entrez Gene 11253
HGNC 6823
MIM 604346
UniProt Q9UKM7  
 MAN1B1 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for MAN1B1
Molecular Function
    calcium ion binding GO:0005509 TAS
    mannosyl-oligosaccharide 1,2-alpha-mannosidase activity GO:0004571 TAS
Biological Process
    cellular protein metabolic process GO:0044267 TAS
    protein folding GO:0006457 TAS
    post-translational protein modification GO:0043687 TAS
    oligosaccharide metabolic process GO:0009311 TAS
    ER-associated ubiquitin-dependent protein catabolic process GO:0030433 IMP
    protein N-linked glycosylation GO:0006487 NAS
    protein N-linked glycosylation via asparagine GO:0018279 TAS
Subcellular Localization
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum quality control compartment GO:0044322 TAS
    extracellular vesicle GO:1903561 IDA
    endoplasmic reticulum membrane GO:0005789 IEA
    membrane GO:0016020 IDA
    integral component of membrane GO:0016021 TAS
 Experiment description of studies that identified MAN1B1 in exosomes
1
Experiment ID 237
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
2
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MAN1B1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which MAN1B1 is involved
PathwayEvidenceSource
Defective MAN1B1 causes MRT15 TAS Reactome
ER Quality Control Compartment (ERQC) TAS Reactome





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