Gene description for PIGK
Gene name phosphatidylinositol glycan anchor biosynthesis, class K
Gene symbol PIGK
Other names/aliases GPI8
Species Homo sapiens
 Database cross references - PIGK
ExoCarta ExoCarta_10026
Vesiclepedia VP_10026
Entrez Gene 10026
HGNC 8965
MIM 605087
UniProt Q92643  
 PIGK identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for PIGK
Molecular Function
    protein disulfide isomerase activity GO:0003756 TAS
    GPI-anchor transamidase activity GO:0003923 IBA
    GPI-anchor transamidase activity GO:0003923 IMP
    GPI-anchor transamidase activity GO:0003923 TAS
    protein binding GO:0005515 IPI
    GPI anchor binding GO:0034235 TAS
Biological Process
    GPI anchor biosynthetic process GO:0006506 IEA
    proteolysis GO:0006508 IEA
    attachment of GPI anchor to protein GO:0016255 IBA
    attachment of GPI anchor to protein GO:0016255 NAS
    attachment of GPI anchor to protein GO:0016255 TAS
Subcellular Localization
    endoplasmic reticulum membrane GO:0005789 NAS
    endoplasmic reticulum membrane GO:0005789 TAS
    membrane GO:0016020 HDA
    GPI-anchor transamidase complex GO:0042765 IBA
    GPI-anchor transamidase complex GO:0042765 IDA
    GPI-anchor transamidase complex GO:0042765 IMP
    GPI-anchor transamidase complex GO:0042765 IPI
 Experiment description of studies that identified PIGK in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for PIGK
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NME2 4831
Affinity Capture-MS Homo sapiens
2 B3GAT3 26229
Affinity Capture-MS Homo sapiens
3 PRG2  
Affinity Capture-MS Homo sapiens
4 LYPD3 27076
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
6 TMEM106A  
Affinity Capture-MS Homo sapiens
7 KLK15  
Affinity Capture-MS Homo sapiens
8 GPAA1 8733
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 FAM20C 56975
Affinity Capture-MS Homo sapiens
10 CANX 821
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 RAB7A 7879
Proximity Label-MS Homo sapiens
12 VAPA 9218
Affinity Capture-MS Homo sapiens
13 GP9 2815
Affinity Capture-MS Homo sapiens
14 PGRMC1 10857
Affinity Capture-MS Homo sapiens
15 CALR3  
Proximity Label-MS Homo sapiens
16 PDIA4 9601
Proximity Label-MS Homo sapiens
17 ULBP2 80328
Affinity Capture-MS Homo sapiens
18 LRRC59 55379
Proximity Label-MS Homo sapiens
19 PLD6  
Affinity Capture-MS Homo sapiens
20 B3GNT3 10331
Affinity Capture-MS Homo sapiens
21 CPNE2 221184
Affinity Capture-MS Homo sapiens
22 HS2ST1 9653
Affinity Capture-MS Homo sapiens
23 PRSS50  
Affinity Capture-MS Homo sapiens
24 TSPAN15 23555
Affinity Capture-MS Homo sapiens
25 KIF20A 10112
Affinity Capture-MS Homo sapiens
26 PIGU 128869
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 PARK2  
Affinity Capture-MS Homo sapiens
28 FBXO6 26270
Affinity Capture-MS Homo sapiens
29 PLD4  
Affinity Capture-MS Homo sapiens
30 COPE 11316
Affinity Capture-MS Homo sapiens
31 LPAR1 1902
Affinity Capture-MS Homo sapiens
32 HSPA5 3309
Affinity Capture-MS Homo sapiens
33 HSP90AA4P 3323
Affinity Capture-MS Homo sapiens
34 PLD3 23646
Affinity Capture-MS Homo sapiens
35 TMED10 10972
Affinity Capture-MS Homo sapiens
36 IDS  
Affinity Capture-MS Homo sapiens
37 PRKCA 5578
Affinity Capture-MS Homo sapiens
38 RPA3 6119
Proximity Label-MS Homo sapiens
39 FBXO2 26232
Affinity Capture-MS Homo sapiens
40 PIGT 51604
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 PSCA 8000
Affinity Capture-MS Homo sapiens
42 ALPP 250
Reconstituted Complex Homo sapiens
43 CLEC2B  
Affinity Capture-MS Homo sapiens
44 RPN1 6184
Proximity Label-MS Homo sapiens
45 P2RX2  
Affinity Capture-MS Homo sapiens
46 CST9L  
Affinity Capture-MS Homo sapiens
47 PIGS 94005
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
48 ST8SIA5  
Affinity Capture-MS Homo sapiens
49 NR3C1 2908
Proximity Label-MS Homo sapiens
50 THY1 7070
Affinity Capture-MS Homo sapiens
51 VAPB 9217
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 CCDC47 57003
Affinity Capture-MS Homo sapiens
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