Gene description for ULBP2
Gene name UL16 binding protein 2
Gene symbol ULBP2
Other names/aliases ALCAN-alpha
N2DL2
NKG2DL2
RAET1H
Species Homo sapiens
 Database cross references - ULBP2
ExoCarta ExoCarta_80328
Vesiclepedia VP_80328
Entrez Gene 80328
HGNC 14894
MIM 605698
UniProt Q9BZM5  
 ULBP2 identified in exosomes derived from the following tissue/cell type
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
 Gene ontology annotations for ULBP2
Molecular Function
    protein binding GO:0005515 IPI
    natural killer cell lectin-like receptor binding GO:0046703 IDA
    receptor ligand activity GO:0048018 IDA
Biological Process
    positive regulation of T cell mediated cytotoxicity GO:0001916 IBA
    antigen processing and presentation of endogenous peptide antigen via MHC class Ib GO:0002476 IBA
    antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-independent GO:0002486 IBA
    immune response GO:0006955 IBA
    signal transduction GO:0007165 IEA
    natural killer cell activation GO:0030101 IDA
    natural killer cell mediated cytotoxicity GO:0042267 IDA
Subcellular Localization
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IDA
    endoplasmic reticulum GO:0005783 IEA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    external side of plasma membrane GO:0009897 IBA
    cell surface GO:0009986 IDA
    intracellular membrane-bounded organelle GO:0043231 IDA
 Experiment description of studies that identified ULBP2 in exosomes
1
Experiment ID 363
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 364
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 365
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for ULBP2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TNFSF8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 HOXB5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 MED21  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 DUSP22  
Affinity Capture-MS Homo sapiens
5 RAET1E  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 PDGFRA 5156
Two-hybrid Homo sapiens
7 TMEM30B 161291
Affinity Capture-MS Homo sapiens
8 RAET1L  
Affinity Capture-MS Homo sapiens
9 SLAMF8  
Affinity Capture-MS Homo sapiens
10 SLC39A5 283375
Affinity Capture-MS Homo sapiens
11 DUS4L  
Affinity Capture-MS Homo sapiens
12 MTUS2 23281
Affinity Capture-MS Homo sapiens
13 CGRRF1  
Affinity Capture-MS Homo sapiens
14 PIGK 10026
Affinity Capture-MS Homo sapiens
15 H2AFZ 3015
Cross-Linking-MS (XL-MS) Homo sapiens
16 GJB7  
Affinity Capture-MS Homo sapiens
17 YIPF6 286451
Two-hybrid Homo sapiens
18 IDO2  
Affinity Capture-MS Homo sapiens
19 ANKRD12  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 SLC4A8 9498
Affinity Capture-MS Homo sapiens
21 FCGRT 2217
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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