Gene description for PIGU
Gene name phosphatidylinositol glycan anchor biosynthesis, class U
Gene symbol PIGU
Other names/aliases CDC91L1
GAB1
Species Homo sapiens
 Database cross references - PIGU
ExoCarta ExoCarta_128869
Vesiclepedia VP_128869
Entrez Gene 128869
HGNC 15791
MIM 608528
UniProt Q9H490  
 PIGU identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for PIGU
Molecular Function
    protein binding GO:0005515 IPI
    GPI anchor binding GO:0034235 IMP
Biological Process
    GPI anchor biosynthetic process GO:0006506 IDA
    GPI anchor biosynthetic process GO:0006506 IEA
    attachment of GPI anchor to protein GO:0016255 IBA
    attachment of GPI anchor to protein GO:0016255 IMP
    attachment of GPI anchor to protein GO:0016255 NAS
    attachment of GPI anchor to protein GO:0016255 TAS
    regulation of receptor signaling pathway via JAK-STAT GO:0046425 IDA
Subcellular Localization
    endoplasmic reticulum membrane GO:0005789 NAS
    endoplasmic reticulum membrane GO:0005789 TAS
    plasma membrane GO:0005886 IDA
    membrane GO:0016020 HDA
    GPI-anchor transamidase complex GO:0042765 IBA
    GPI-anchor transamidase complex GO:0042765 IDA
    GPI-anchor transamidase complex GO:0042765 IPI
 Experiment description of studies that identified PIGU in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for PIGU
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CDH13 1012
Affinity Capture-MS Homo sapiens
2 NRG1 3084
Affinity Capture-MS Homo sapiens
3 F2RL1  
Two-hybrid Homo sapiens
4 CLEC5A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 FNDC4 64838
Affinity Capture-MS Homo sapiens
6 GJD3  
Proximity Label-MS Homo sapiens
7 BCAP31 10134
Proximity Label-MS Homo sapiens
8 LYPD3 27076
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 VNN2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 SLC6A15 55117
Affinity Capture-MS Homo sapiens
11 GLRA2  
Affinity Capture-MS Homo sapiens
12 GPAA1 8733
Co-fractionation Homo sapiens
13 RPN2 6185
Proximity Label-MS Homo sapiens
14 TSPAN17 26262
Affinity Capture-MS Homo sapiens
15 GPR17 2840
Affinity Capture-MS Homo sapiens
16 CMTM5  
Affinity Capture-MS Homo sapiens
17 RNF121 55298
Affinity Capture-Western Homo sapiens
18 TXNDC15  
Affinity Capture-MS Homo sapiens
19 SYNGR1 9145
Affinity Capture-MS Homo sapiens
20 NPRL3  
Affinity Capture-MS Homo sapiens
21 RTP2  
Affinity Capture-MS Homo sapiens
22 GAL3ST1  
Affinity Capture-MS Homo sapiens
23 RAMP3  
Affinity Capture-MS Homo sapiens
24 NIPAL1  
Affinity Capture-MS Homo sapiens
25 CHRNA10  
Affinity Capture-MS Homo sapiens
26 B3GNT3 10331
Affinity Capture-MS Homo sapiens
27 ERGIC3 51614
Affinity Capture-MS Homo sapiens
28 HS2ST1 9653
Affinity Capture-MS Homo sapiens
29 PRSS50  
Affinity Capture-MS Homo sapiens
30 TSPAN15 23555
Affinity Capture-MS Homo sapiens
31 NR3C1 2908
Proximity Label-MS Homo sapiens
32 GCGR  
Affinity Capture-MS Homo sapiens
33 HSD17B11 51170
Proximity Label-MS Homo sapiens
34 EMD 2010
Proximity Label-MS Homo sapiens
35 PIGK 10026
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 FKBP8 23770
Proximity Label-MS Homo sapiens
37 HSD3B7 80270
Proximity Label-MS Homo sapiens
38 KLRC2  
Affinity Capture-MS Homo sapiens
39 CLPP 8192
Proximity Label-MS Homo sapiens
40 DNAJC25 548645
Proximity Label-MS Homo sapiens
41 ASPH 444
Affinity Capture-MS Homo sapiens
42 ERGIC1 57222
Proximity Label-MS Homo sapiens
43 P2RY8  
Affinity Capture-MS Homo sapiens
44 FFAR1  
Affinity Capture-MS Homo sapiens
45 LPAR1 1902
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 P2RY2 5029
Affinity Capture-MS Homo sapiens
47 AP1G2 8906
Affinity Capture-MS Homo sapiens
48 GPR35  
Affinity Capture-MS Homo sapiens
49 GPR45  
Affinity Capture-MS Homo sapiens
50 SCN4A  
Affinity Capture-MS Homo sapiens
51 ART4  
Affinity Capture-MS Homo sapiens
52 EMR4P  
Affinity Capture-MS Homo sapiens
53 CCDC155  
Two-hybrid Homo sapiens
54 PIGN 23556
Affinity Capture-MS Homo sapiens
55 CLEC4E  
Affinity Capture-MS Homo sapiens
56 SEC62 7095
Proximity Label-MS Homo sapiens
57 SPPL2B 56928
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 KITLG  
Affinity Capture-MS Homo sapiens
59 PIGT 51604
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 ACKR2  
Affinity Capture-MS Homo sapiens
61 FBXO2 26232
Affinity Capture-MS Homo sapiens
62 IFI6  
Affinity Capture-MS Homo sapiens
63 PRSS8 5652
Affinity Capture-MS Homo sapiens
64 RAB7A 7879
Proximity Label-MS Homo sapiens
65 STOM 2040
Affinity Capture-MS Homo sapiens
66 RPN1 6184
Proximity Label-MS Homo sapiens
67 DHFRL1  
Proximity Label-MS Homo sapiens
68 PVRL4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 EDNRA 1909
Affinity Capture-MS Homo sapiens
70 CRELD1 78987
Affinity Capture-MS Homo sapiens
71 PIGS 94005
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
72 VIPR1  
Affinity Capture-MS Homo sapiens
73 SSR1 6745
Proximity Label-MS Homo sapiens
74 SLC22A4 6583
Affinity Capture-MS Homo sapiens
75 PML 5371
Affinity Capture-MS Homo sapiens
76 GPR173  
Affinity Capture-MS Homo sapiens
77 AGTR1  
Affinity Capture-MS Homo sapiens
78 THY1 7070
Affinity Capture-MS Homo sapiens
79 SEC61B 10952
Proximity Label-MS Homo sapiens
80 VAPB 9217
Affinity Capture-MS Homo sapiens
81 DNAJC16  
Proximity Label-MS Homo sapiens
82 YIPF3 25844
Affinity Capture-MS Homo sapiens
83 CCDC47 57003
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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