Gene description for RNF121
Gene name ring finger protein 121
Gene symbol RNF121
Other names/aliases -
Species Homo sapiens
 Database cross references - RNF121
ExoCarta ExoCarta_55298
Vesiclepedia VP_55298
Entrez Gene 55298
HGNC 21070
UniProt Q9H920  
 RNF121 identified in exosomes derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for RNF121
Molecular Function
    metal ion binding GO:0046872 IEA
    ubiquitin protein ligase activity GO:0061630 IBA
Biological Process
    protein ubiquitination GO:0016567 IEA
    ERAD pathway GO:0036503 IBA
Subcellular Localization
    Golgi membrane GO:0000139 IBA
    endoplasmic reticulum membrane GO:0005789 IBA
 Experiment description of studies that identified RNF121 in exosomes
1
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for RNF121
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 PDE2A  
Affinity Capture-MS Homo sapiens
3 NFKBIA  
Affinity Capture-Western Homo sapiens
4 EDEM1  
Affinity Capture-MS Homo sapiens
5 BSCL2  
Affinity Capture-MS Homo sapiens
6 CACNG6  
Affinity Capture-MS Homo sapiens
7 PIGU 128869
Affinity Capture-Western Homo sapiens
8 RAB7A 7879
Proximity Label-MS Homo sapiens
9 LPAR1 1902
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which RNF121 is involved
No pathways found





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