Gene description for CHCHD6
Gene name coiled-coil-helix-coiled-coil-helix domain containing 6
Gene symbol CHCHD6
Other names/aliases CHCM1
PPP1R23
Species Homo sapiens
 Database cross references - CHCHD6
ExoCarta ExoCarta_84303
Vesiclepedia VP_84303
Entrez Gene 84303
HGNC 28184
MIM 615634
UniProt Q9BRQ6  
 CHCHD6 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
 Gene ontology annotations for CHCHD6
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    DNA damage response GO:0006974 IBA
    DNA damage response GO:0006974 IDA
    inner mitochondrial membrane organization GO:0007007 IC
    cristae formation GO:0042407 IBA
    cristae formation GO:0042407 IMP
    cristae formation GO:0042407 NAS
Subcellular Localization
    SAM complex GO:0001401 HDA
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    mitochondrion GO:0005739 IMP
    mitochondrial inner membrane GO:0005743 IDA
    mitochondrial inner membrane GO:0005743 NAS
    cytosol GO:0005829 IDA
    mitochondrial crista junction GO:0044284 NAS
    MICOS complex GO:0061617 HDA
    MICOS complex GO:0061617 IDA
    MICOS complex GO:0061617 NAS
    MIB complex GO:0140275 HDA
 Experiment description of studies that identified CHCHD6 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 426
MISEV standards
Biophysical techniques
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
CANX
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 427
MISEV standards
Biophysical techniques
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
CANX
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 419
MISEV standards
Biophysical techniques
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
CANX
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 419
MISEV standards
Biophysical techniques
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
CANX
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 420
MISEV standards
Biophysical techniques
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
CANX
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 420
MISEV standards
Biophysical techniques
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
CANX
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 434
MISEV standards
Biophysical techniques
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
CANX
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 435
MISEV standards
Biophysical techniques
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
CANX
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CHCHD6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 IMMT 10989
Affinity Capture-Western Homo sapiens
2 MAGEA8  
Affinity Capture-MS Homo sapiens
3 INVS  
Affinity Capture-MS Homo sapiens
4 ANLN 54443
Affinity Capture-MS Homo sapiens
5 CLEC5A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 Tmed2 56334
Affinity Capture-MS Mus musculus
7 SLC22A16 85413
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
9 PHB2 11331
Proximity Label-MS Homo sapiens
10 NAAA  
Affinity Capture-MS Homo sapiens
11 SLC2A1 6513
Affinity Capture-MS Homo sapiens
12 APLNR  
Affinity Capture-MS Homo sapiens
13 KIF14 9928
Affinity Capture-MS Homo sapiens
14 Erh  
Affinity Capture-MS Mus musculus
15 SLC25A12 8604
Proximity Label-MS Homo sapiens
Co-fractionation Homo sapiens
16 TRAK1 22906
Affinity Capture-MS Homo sapiens
17 AIFM1 9131
Proximity Label-MS Homo sapiens
18 Mtx2  
Affinity Capture-MS Mus musculus
19 C19orf52  
Proximity Label-MS Homo sapiens
20 VAPA 9218
Affinity Capture-MS Homo sapiens
21 COX15 1355
Proximity Label-MS Homo sapiens
22 CMTM8  
Affinity Capture-MS Homo sapiens
23 MFSD4  
Affinity Capture-MS Homo sapiens
24 MTX2 10651
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 SLC2A4  
Affinity Capture-MS Homo sapiens
26 Aifm1  
Affinity Capture-MS Mus musculus
27 PTH2R  
Affinity Capture-MS Homo sapiens
28 Bmpr1a  
Affinity Capture-MS Mus musculus
29 PCNP 57092
Affinity Capture-MS Homo sapiens
30 GOLT1B 51026
Affinity Capture-MS Homo sapiens
31 Rmdn3  
Affinity Capture-MS Mus musculus
32 MGARP  
Affinity Capture-MS Homo sapiens
33 SAMM50 25813
Proximity Label-MS Homo sapiens
34 SPSB4  
Affinity Capture-MS Homo sapiens
35 KIF20A 10112
Affinity Capture-MS Homo sapiens
36 UBC 7316
Affinity Capture-MS Homo sapiens
37 FOXRED2  
Affinity Capture-MS Homo sapiens
38 TMED4 222068
Co-fractionation Homo sapiens
39 FAXC  
Affinity Capture-MS Homo sapiens
40 SLC2A8 29988
Affinity Capture-MS Homo sapiens
41 NDUFA8 4702
Co-fractionation Homo sapiens
42 APP 351
Reconstituted Complex Homo sapiens
43 HACD3 51495
Co-fractionation Homo sapiens
44 MMGT1 93380
Affinity Capture-MS Homo sapiens
45 SFXN1 94081
Proximity Label-MS Homo sapiens
46 APOO 79135
Proximity Label-MS Homo sapiens
47 DIABLO 56616
Proximity Label-MS Homo sapiens
48 MFSD11  
Affinity Capture-MS Homo sapiens
49 CHCHD2  
Co-fractionation Homo sapiens
50 CHCHD3 54927
Affinity Capture-MS Homo sapiens
51 APOOL 139322
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 ARL4D  
Affinity Capture-MS Homo sapiens
53 KIF23 9493
Affinity Capture-MS Homo sapiens
54 RAB7A 7879
Affinity Capture-MS Homo sapiens
55 SLC25A51  
Proximity Label-MS Homo sapiens
56 C19orf70  
Affinity Capture-MS Homo sapiens
57 GPR182  
Affinity Capture-MS Homo sapiens
58 OPA1 4976
Proximity Label-MS Homo sapiens
59 CD244 51744
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 ERLIN1 10613
Co-fractionation Homo sapiens
61 PRC1 9055
Affinity Capture-MS Homo sapiens
62 ZER1 10444
Affinity Capture-MS Homo sapiens
63 Tnpo1 238799
Affinity Capture-MS Mus musculus
64 B3GNT2 10678
Affinity Capture-MS Homo sapiens
65 PML 5371
Affinity Capture-MS Homo sapiens
66 ISLR 3671
Affinity Capture-MS Homo sapiens
67 S1PR1 1901
Affinity Capture-MS Homo sapiens
68 SLC1A1 6505
Affinity Capture-MS Homo sapiens
69 DNAJC30  
Proximity Label-MS Homo sapiens
70 P2RY8  
Affinity Capture-MS Homo sapiens
71 SURF1  
Proximity Label-MS Homo sapiens
72 FFAR1  
Affinity Capture-MS Homo sapiens
73 LRRN4CL 221091
Affinity Capture-MS Homo sapiens
74 TRAK2 66008
Affinity Capture-MS Homo sapiens
75 COX4I1 1327
Proximity Label-MS Homo sapiens
View the network image/svg+xml
 Pathways in which CHCHD6 is involved
PathwayEvidenceSource
Cristae formation IEA Reactome
Mitochondrial biogenesis IEA Reactome
Organelle biogenesis and maintenance IEA Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here