Gene description for HACD2
Gene name 3-hydroxyacyl-CoA dehydratase 2
Gene symbol HACD2
Other names/aliases PTPLB
Species Homo sapiens
 Database cross references - HACD2
ExoCarta ExoCarta_201562
Vesiclepedia VP_201562
Entrez Gene 201562
HGNC 9640
MIM 615939
UniProt Q6Y1H2  
 HACD2 identified in exosomes derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for HACD2
Molecular Function
    protein tyrosine phosphatase activity GO:0004725 IMP
    protein binding GO:0005515 IPI
    3-hydroxyacyl-CoA dehydratase activity GO:0018812 EXP
    3-hydroxyacyl-CoA dehydratase activity GO:0018812 IBA
    enzyme binding GO:0019899 IDA
    very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase activity GO:0102158 IDA
Biological Process
    sphingolipid biosynthetic process GO:0030148 IBA
    sphingolipid biosynthetic process GO:0030148 IGI
    fatty acid elongation GO:0030497 IBA
    fatty acid elongation GO:0030497 IDA
    long-chain fatty-acyl-CoA biosynthetic process GO:0035338 TAS
    very long-chain fatty acid biosynthetic process GO:0042761 IBA
    very long-chain fatty acid biosynthetic process GO:0042761 IDA
Subcellular Localization
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum membrane GO:0005789 IBA
    endoplasmic reticulum membrane GO:0005789 TAS
 Experiment description of studies that identified HACD2 in exosomes
1
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for HACD2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 MAGEA8  
Affinity Capture-MS Homo sapiens
3 SLC2A12 154091
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 LDHA 3939
Cross-Linking-MS (XL-MS) Homo sapiens
5 SFT2D1 113402
Affinity Capture-MS Homo sapiens
6 BSCL2  
Affinity Capture-MS Homo sapiens
7 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
8 FASN 2194
Negative Genetic Homo sapiens
9 VIPR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 ATP13A3 79572
Affinity Capture-MS Homo sapiens
11 APLNR  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 SLC18A1  
Affinity Capture-MS Homo sapiens
13 TACR1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 TTYH1  
Affinity Capture-MS Homo sapiens
15 GPR17 2840
Affinity Capture-MS Homo sapiens
16 TP53 7157
Affinity Capture-MS Homo sapiens
17 SPAG6  
Affinity Capture-MS Homo sapiens
18 Cct3 12462
Affinity Capture-MS Mus musculus
19 C3AR1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 VTN 7448
Affinity Capture-MS Homo sapiens
21 SLC22A4 6583
Affinity Capture-MS Homo sapiens
22 SMIM1 388588
Two-hybrid Homo sapiens
23 PTH2R  
Affinity Capture-MS Homo sapiens
24 SLC26A6 65010
Affinity Capture-MS Homo sapiens
25 SLC39A4 55630
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 ASGR2  
Affinity Capture-MS Homo sapiens
27 BCAP31 10134
PCA Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
28 SLC1A5 6510
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 GCGR  
Affinity Capture-MS Homo sapiens
30 PBRM1 55193
Affinity Capture-MS Homo sapiens
31 UBC 7316
Reconstituted Complex Homo sapiens
32 B4GAT1 11041
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
34 NIPAL1  
Affinity Capture-MS Homo sapiens
35 ADCY8  
Affinity Capture-MS Homo sapiens
36 OPRL1 4987
Affinity Capture-MS Homo sapiens
37 LPAR1 1902
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 TTC30B  
Affinity Capture-MS Homo sapiens
39 Max  
Affinity Capture-MS Mus musculus
40 FAM209A 200232
Two-hybrid Homo sapiens
41 SLC18A2  
Affinity Capture-MS Homo sapiens
42 C12orf49  
Negative Genetic Homo sapiens
43 SLC11A2 4891
Affinity Capture-MS Homo sapiens
44 Tpx2  
Affinity Capture-MS Mus musculus
45 ATP2A1 487
Affinity Capture-MS Homo sapiens
46 SPPL2B 56928
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 CASP6 839
Affinity Capture-MS Homo sapiens
48 GPR182  
Affinity Capture-MS Homo sapiens
49 ORAI1 84876
Affinity Capture-MS Homo sapiens
50 SLC6A15 55117
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 IPPK  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 ALDH1A3 220
Affinity Capture-MS Homo sapiens
53 VIPR1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 P2RY10  
Affinity Capture-MS Homo sapiens
55 TECR 9524
Two-hybrid Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
56 SLC1A1 6505
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 KCNA2  
Affinity Capture-MS Homo sapiens
58 ATP2A3 489
Affinity Capture-MS Homo sapiens
59 FPR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 OPALIN  
Affinity Capture-MS Homo sapiens
61 CREB3L1  
Two-hybrid Homo sapiens
62 TMX2 51075
Two-hybrid Homo sapiens
View the network image/svg+xml



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