Gene description for SLC20A1
Gene name solute carrier family 20 (phosphate transporter), member 1
Gene symbol SLC20A1
Other names/aliases GLVR1
Glvr-1
PIT1
PiT-1
Species Homo sapiens
 Database cross references - SLC20A1
ExoCarta ExoCarta_6574
Vesiclepedia VP_6574
Entrez Gene 6574
HGNC 10946
MIM 137570
UniProt Q8WUM9  
 SLC20A1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Melanoma cells 25950383    
Melanoma cells 25950383    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for SLC20A1
Molecular Function
    phosphate transmembrane transporter activity GO:0005315 IBA
    high-affinity phosphate:sodium symporter activity GO:0005316 IEA
    sodium:phosphate symporter activity GO:0005436 IDA
    sodium:phosphate symporter activity GO:0005436 TAS
    signaling receptor activity GO:0038023 TAS
Biological Process
    phosphate-containing compound metabolic process GO:0006796 TAS
    monoatomic ion transport GO:0006811 TAS
    cell population proliferation GO:0008283 IDA
    biomineral tissue development GO:0031214 IEA
    phosphate ion transmembrane transport GO:0035435 IBA
    sodium ion transmembrane transport GO:0035725 IEA
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 HMP
Subcellular Localization
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    membrane GO:0016020 TAS
 Experiment description of studies that identified SLC20A1 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 207
MISEV standards
EM
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 208
MISEV standards
EM
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 209
MISEV standards
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 259
MISEV standards
EM
Biophysical techniques
TSG101|FLOT1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name A375M
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 260
MISEV standards
EM
Biophysical techniques
TSG101|FLOT1|CD81
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 1205Lu
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for SLC20A1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MPP6 51678
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 RNF149 284996
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 UPK2 7379
Affinity Capture-MS Homo sapiens
4 SLC26A2 1836
Affinity Capture-MS Homo sapiens
5 RAMP3  
Affinity Capture-MS Homo sapiens
6 MCAM 4162
Proximity Label-MS Homo sapiens
7 KLRB1  
Affinity Capture-MS Homo sapiens
8 FASN 2194
Positive Genetic Homo sapiens
9 FLVCR1 28982
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 IZUMO1  
Affinity Capture-MS Homo sapiens
11 TMEM9 252839
Affinity Capture-MS Homo sapiens
12 PCDHB7  
Affinity Capture-MS Homo sapiens
13 CTDSP1 58190
Affinity Capture-MS Homo sapiens
14 TGFBR2 7048
Affinity Capture-MS Homo sapiens
15 TSPAN15 23555
Affinity Capture-MS Homo sapiens
16 ICAM2 3384
Affinity Capture-MS Homo sapiens
17 RNF11 26994
Affinity Capture-MS Homo sapiens
18 LAMP3  
Proximity Label-MS Homo sapiens
19 SYNGR1 9145
Affinity Capture-MS Homo sapiens
20 KCNE3  
Affinity Capture-MS Homo sapiens
21 CD83  
Affinity Capture-MS Homo sapiens
22 VSIG4  
Affinity Capture-MS Homo sapiens
23 NDFIP1 80762
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 SLC20A2 6575
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 CSNK1G2 1455
Affinity Capture-MS Homo sapiens
26 ARRDC4  
Affinity Capture-MS Homo sapiens
27 BMPR1A 657
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 CX3CL1  
Affinity Capture-MS Homo sapiens
29 GPR12  
Affinity Capture-MS Homo sapiens
30 KCNC3 3748
Affinity Capture-MS Homo sapiens
31 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
32 VHL  
Negative Genetic Homo sapiens
33 DNAJC5 80331
Proximity Label-MS Homo sapiens
34 ARF6 382
Proximity Label-MS Homo sapiens
35 TIGIT  
Affinity Capture-MS Homo sapiens
36 BCL10  
Two-hybrid Homo sapiens
37 UGCG 7357
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 ARMC6 93436
Affinity Capture-MS Homo sapiens
39 FFAR1  
Affinity Capture-MS Homo sapiens
40 ELOVL5 60481
Proximity Label-MS Homo sapiens
41 SPACA1 81833
Affinity Capture-MS Homo sapiens
42 NXF1 10482
Affinity Capture-RNA Homo sapiens
43 LIN7A 8825
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 SYP  
Affinity Capture-MS Homo sapiens
45 ADCY9 115
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 TSPAN5 10098
Affinity Capture-MS Homo sapiens
47 TNFRSF10B 8795
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 MAPK6  
Two-hybrid Homo sapiens
49 B3GAT1  
Proximity Label-MS Homo sapiens
50 ARL5B  
Affinity Capture-MS Homo sapiens
51 LIN7C 55327
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 SLC9A1 6548
Affinity Capture-MS Homo sapiens
53 HLA-B 3106
Affinity Capture-MS Homo sapiens
54 TMEM150A  
Affinity Capture-MS Homo sapiens
55 SLC22A18 5002
Affinity Capture-MS Homo sapiens
56 TNFRSF10C  
Affinity Capture-MS Homo sapiens
57 CUL7 9820
Affinity Capture-MS Homo sapiens
58 KRAS 3845
Proximity Label-MS Homo sapiens
59 MTX3  
Affinity Capture-MS Homo sapiens
60 VIPR1  
Affinity Capture-MS Homo sapiens
61 MPP2 4355
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 NOTCH3 4854
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 SLC1A1 6505
Affinity Capture-MS Homo sapiens
64 RAPGEF1 2889
Two-hybrid Homo sapiens
65 ACVR2B  
Affinity Capture-MS Homo sapiens
66 TMEM171  
Affinity Capture-MS Homo sapiens
67 LRP10 26020
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 MFAP3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 SLC22A9  
Affinity Capture-MS Homo sapiens
70 SYT11 23208
Affinity Capture-MS Homo sapiens
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