Gene description for CERS1
Gene name ceramide synthase 1
Gene symbol CERS1
Other names/aliases EPM8
LAG1
LASS1
UOG1
Species Homo sapiens
 Database cross references - CERS1
ExoCarta ExoCarta_10715
Vesiclepedia VP_10715
Entrez Gene 10715
HGNC 14253
MIM 606919
UniProt P27544  
 CERS1 identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells Unpublished / Not applicable
 Gene ontology annotations for CERS1
Molecular Function
    sphingosine N-acyltransferase activity GO:0050291 IBA
    sphingosine N-acyltransferase activity GO:0050291 IDA
    sphingosine N-acyltransferase activity GO:0050291 ISS
    sphingosine N-acyltransferase activity GO:0050291 TAS
Biological Process
    sphingolipid biosynthetic process GO:0030148 IDA
    sphingolipid biosynthetic process GO:0030148 TAS
    cellular response to mycotoxin GO:0036146 IDA
    ceramide biosynthetic process GO:0046513 IBA
    ceramide biosynthetic process GO:0046513 IDA
    ceramide biosynthetic process GO:0046513 ISS
    cellular response to xenobiotic stimulus GO:0071466 IDA
    cellular response to UV-A GO:0071492 IDA
    cellular response to dithiothreitol GO:0072721 IDA
    positive regulation of mitophagy GO:1901526 IDA
Subcellular Localization
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum membrane GO:0005789 TAS
    membrane GO:0016020 TAS
    intracellular membrane-bounded organelle GO:0043231 ISS
 Experiment description of studies that identified CERS1 in sEVs
1
Experiment ID 126
MISEV standards
Biophysical techniques
GAPDH
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Antibody array
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
 Protein-protein interactions for CERS1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 VCAM1 7412
Affinity Capture-MS Homo sapiens
2 RPN1 6184
Proximity Label-MS Homo sapiens
3 RAB5C 5878
Proximity Label-MS Homo sapiens
4 MAPK6  
Two-hybrid Homo sapiens
5 BDKRB1 623
Two-hybrid Homo sapiens
6 SSR1 6745
Proximity Label-MS Homo sapiens
7 PLD3 23646
Affinity Capture-MS Homo sapiens
8 RPN2 6185
Proximity Label-MS Homo sapiens
9 HTR4  
Two-hybrid Homo sapiens
10 SEC61B 10952
Proximity Label-MS Homo sapiens
11 METTL7A 25840
Proximity Label-MS Homo sapiens
12 LTB4R2  
Two-hybrid Homo sapiens
13 DRD2  
Two-hybrid Homo sapiens
14 ATP2A1 487
Proximity Label-MS Homo sapiens
View the network image/svg+xml
 Pathways in which CERS1 is involved
PathwayEvidenceSource
Metabolism TAS Reactome
Metabolism of lipids TAS Reactome
Sphingolipid de novo biosynthesis TAS Reactome
Sphingolipid metabolism TAS Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here