Gene description for RNASEL
Gene name ribonuclease L (2',5'-oligoisoadenylate synthetase-dependent)
Gene symbol RNASEL
Other names/aliases PRCA1
RNS4
Species Homo sapiens
 Database cross references - RNASEL
ExoCarta ExoCarta_6041
Vesiclepedia VP_6041
Entrez Gene 6041
HGNC 10050
MIM 180435
UniProt Q05823  
 RNASEL identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for RNASEL
Molecular Function
    RNA binding GO:0003723 IBA
    RNA binding GO:0003723 IDA
    RNA endonuclease activity GO:0004521 NAS
    RNA nuclease activity GO:0004540 IBA
    RNA nuclease activity GO:0004540 IDA
    protein kinase activity GO:0004672 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    rRNA binding GO:0019843 IEA
    identical protein binding GO:0042802 IPI
    ribonucleoprotein complex binding GO:0043021 IEA
    metal ion binding GO:0046872 IEA
Biological Process
    rRNA processing GO:0006364 IEA
    RNA processing GO:0006396 IBA
    mRNA processing GO:0006397 IEA
    protein phosphorylation GO:0006468 NAS
    regulation of mRNA stability GO:0043488 IEA
    negative regulation of viral genome replication GO:0045071 IBA
    negative regulation of viral genome replication GO:0045071 IDA
    fat cell differentiation GO:0045444 IEA
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    positive regulation of D-glucose import GO:0046326 IEA
    defense response to virus GO:0051607 IBA
    defense response to virus GO:0051607 IDA
Subcellular Localization
    mitochondrial matrix GO:0005759 TAS
    cytosol GO:0005829 TAS
    nuclear matrix GO:0016363 IEA
 Experiment description of studies that identified RNASEL in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for RNASEL
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SLC37A3  
Affinity Capture-MS Homo sapiens
2 IL13RA2 3598
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 SLC15A1  
Affinity Capture-MS Homo sapiens
4 SV2A 9900
Affinity Capture-MS Homo sapiens
5 PRKY  
Affinity Capture-MS Homo sapiens
6 TP53 7157
Affinity Capture-MS Homo sapiens
7 DOCK5 80005
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 HSD17B1  
Affinity Capture-MS Homo sapiens
9 C1QA 712
Affinity Capture-MS Homo sapiens
10 PTH2R  
Affinity Capture-MS Homo sapiens
11 S100A2 6273
Affinity Capture-MS Homo sapiens
12 SLC5A6 8884
Affinity Capture-MS Homo sapiens
13 PRSS42  
Affinity Capture-MS Homo sapiens
14 HNRNPCL2 440563
Affinity Capture-MS Homo sapiens
15 SYP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 SLC18A2  
Affinity Capture-MS Homo sapiens
17 HIF1AN 55662
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 TCF12  
Two-hybrid Homo sapiens
19 LPAR4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 NXPH1  
Affinity Capture-MS Homo sapiens
21 ABCE1 6059
Reconstituted Complex Homo sapiens
22 ST8SIA4 7903
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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