Gene description for IFIT2
Gene name interferon-induced protein with tetratricopeptide repeats 2
Gene symbol IFIT2
Other names/aliases G10P2
GARG-39
IFI-54
IFI-54K
IFI54
IFIT-2
ISG-54 K
ISG-54K
ISG54
P54
cig42
Species Homo sapiens
 Database cross references - IFIT2
ExoCarta ExoCarta_3433
Vesiclepedia VP_3433
Entrez Gene 3433
HGNC 5409
MIM 147040
UniProt P09913  
 IFIT2 identified in sEVs derived from the following tissue/cell type
Endothelial cells 26027894    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
 Gene ontology annotations for IFIT2
Molecular Function
    RNA binding GO:0003723 HDA
    protein binding GO:0005515 IPI
Biological Process
    apoptotic mitochondrial changes GO:0008637 TAS
    response to virus GO:0009615 IDA
    response to virus GO:0009615 IMP
    negative regulation of protein binding GO:0032091 IDA
    positive regulation of apoptotic process GO:0043065 IDA
    defense response to virus GO:0051607 IBA
    antiviral innate immune response GO:0140374 IDA
Subcellular Localization
    cytoplasm GO:0005737 IDA
    endoplasmic reticulum GO:0005783 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified IFIT2 in sEVs
1
Experiment ID 226
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD9|FLOT1
Enriched markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
✘ Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
2
Experiment ID 226
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD9|FLOT1
Enriched markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
✘ Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
3
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for IFIT2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SNRPB2 6629
Affinity Capture-MS Homo sapiens
2 POLG2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 YBX1 4904
Affinity Capture-MS Homo sapiens
4 HNRNPDL 9987
Affinity Capture-MS Homo sapiens
5 HNRNPD 3184
Affinity Capture-MS Homo sapiens
6 ISG15 9636
Affinity Capture-MS Homo sapiens
7 HNRNPL 3191
Affinity Capture-MS Homo sapiens
8 TUBB 203068
Affinity Capture-MS Homo sapiens
9 TOE1 114034
Affinity Capture-MS Homo sapiens
10 SNRPE 6635
Affinity Capture-MS Homo sapiens
11 PABPC4 8761
Affinity Capture-MS Homo sapiens
12 LMAN1 3998
Proximity Label-MS Homo sapiens
13 WWOX 51741
Two-hybrid Homo sapiens
14 DNAJA2 10294
Affinity Capture-MS Homo sapiens
15 SNRPB 6628
Affinity Capture-MS Homo sapiens
16 NUTM1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
17 DHX29 54505
Co-fractionation Homo sapiens
18 HNRNPA2B1 3181
Affinity Capture-MS Homo sapiens
19 SNRPA 6626
Affinity Capture-MS Homo sapiens
20 GATSL3 652968
Affinity Capture-MS Homo sapiens
21 HNRNPA1 3178
Affinity Capture-MS Homo sapiens
22 SNRPF 6636
Affinity Capture-MS Homo sapiens
23 CELF5  
Affinity Capture-MS Homo sapiens
24 SNRPD2 6633
Affinity Capture-MS Homo sapiens
25 ILF2 3608
Affinity Capture-MS Homo sapiens
26 DHX40  
Co-fractionation Homo sapiens
27 WWP2 11060
Two-hybrid Homo sapiens
28 HOMER3 9454
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 HNRNPAB 3182
Affinity Capture-MS Homo sapiens
30 SNRNP70 6625
Affinity Capture-MS Homo sapiens
31 PABPC1 26986
Affinity Capture-MS Homo sapiens
32 SNRPD1 6632
Affinity Capture-MS Homo sapiens
33 IFIT1 3434
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 YBX3 8531
Affinity Capture-MS Homo sapiens
35 RPL31 6160
Affinity Capture-MS Homo sapiens
36 RAB11B 9230
Affinity Capture-MS Homo sapiens
37 RBPMS2  
Two-hybrid Homo sapiens
38 MEOX2  
Two-hybrid Homo sapiens
39 MATR3 9782
Affinity Capture-MS Homo sapiens
40 RCN2 5955
Affinity Capture-MS Homo sapiens
41 RPLP1 6176
Affinity Capture-MS Homo sapiens
42 SNRPC 6631
Affinity Capture-MS Homo sapiens
43 DBT 1629
Affinity Capture-MS Homo sapiens
44 IFIT3 3437
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 DDX58 23586
Affinity Capture-RNA Homo sapiens
46 SNRPA1 6627
Affinity Capture-MS Homo sapiens
47 PHACTR4 65979
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 ILF3 3609
Affinity Capture-MS Homo sapiens
49 BATF3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 SNRPD3 6634
Affinity Capture-MS Homo sapiens
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