Gene description for TPST1
Gene name tyrosylprotein sulfotransferase 1
Gene symbol TPST1
Other names/aliases TANGO13A
Species Homo sapiens
 Database cross references - TPST1
ExoCarta ExoCarta_8460
Vesiclepedia VP_8460
Entrez Gene 8460
HGNC 12020
MIM 603125
UniProt O60507  
 TPST1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for TPST1
Molecular Function
    protein-tyrosine sulfotransferase activity GO:0008476 IBA
    protein-tyrosine sulfotransferase activity GO:0008476 IDA
    protein-tyrosine sulfotransferase activity GO:0008476 TAS
    protein homodimerization activity GO:0042803 IPI
Biological Process
    post-translational protein modification GO:0043687 IDA
    3'-phosphoadenosine 5'-phosphosulfate metabolic process GO:0050427 TAS
Subcellular Localization
    Golgi membrane GO:0000139 IDA
    Golgi membrane GO:0000139 TAS
    Golgi apparatus GO:0005794 IBA
    membrane GO:0016020 TAS
 Experiment description of studies that identified TPST1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for TPST1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GINM1 116254
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 TPST2 8459
Affinity Capture-MS Homo sapiens
3 HSPA5 3309
Affinity Capture-MS Homo sapiens
4 ACTB 60
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 CLEC12B  
Affinity Capture-MS Homo sapiens
6 ADGRE5 976
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 PMCH  
Affinity Capture-MS Homo sapiens
8 GOLPH3L 55204
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
9 CSGALNACT2 55454
Affinity Capture-MS Homo sapiens
10 TMEM132D  
Two-hybrid Homo sapiens
11 GOLPH3 64083
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
12 PDGFRA 5156
Affinity Capture-MS Homo sapiens
13 SCGB2A2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 B2M 567
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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