Gene description for DDX4
Gene name DEAD (Asp-Glu-Ala-Asp) box polypeptide 4
Gene symbol DDX4
Other names/aliases VASA
Species Homo sapiens
 Database cross references - DDX4
ExoCarta ExoCarta_54514
Entrez Gene 54514
HGNC 18700
MIM 605281
UniProt Q9NQI0  
 DDX4 identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for DDX4
Molecular Function
    ATP-dependent RNA helicase activity GO:0004004 IBA
    ATP binding GO:0005524 IEA
    nucleic acid binding GO:0003676 IEA
Biological Process
    piRNA metabolic process GO:0034587 TAS
    spermatogenesis GO:0007283 IEA
    male meiosis I GO:0007141 IEA
    regulation of protein localization GO:0032880 IEA
    RNA secondary structure unwinding GO:0010501 IBA
    multicellular organismal development GO:0007275 IEA
    gene expression GO:0010467 TAS
    sperm motility GO:0030317 IMP
Subcellular Localization
    cytoplasm GO:0005737 IDA
    P granule GO:0043186 IBA
    chromatoid body GO:0033391 IEA
    nucleus GO:0005634 IEA
    pi-body GO:0071546 ISS
    piP-body GO:0071547 ISS
    perinuclear region of cytoplasm GO:0048471 IBA
 Experiment description of studies that identified DDX4 in exosomes
1
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for DDX4
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which DDX4 is involved
PathwayEvidenceSource
PIWI-interacting RNA (piRNA) biogenesis TAS Reactome





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