Gene description for NFIL3
Gene name nuclear factor, interleukin 3 regulated
Gene symbol NFIL3
Other names/aliases E4BP4
IL3BP1
NF-IL3A
NFIL3A
Species Homo sapiens
 Database cross references - NFIL3
ExoCarta ExoCarta_4783
Vesiclepedia VP_4783
Entrez Gene 4783
HGNC 7787
MIM 605327
UniProt Q16649  
 NFIL3 identified in sEVs derived from the following tissue/cell type
Prostate cancer cells 25844599    
 Gene ontology annotations for NFIL3
Molecular Function
    RNA polymerase II transcription regulatory region sequence-specific DNA binding GO:0000977 IDA
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IDA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 ISA
    DNA-binding transcription repressor activity, RNA polymerase II-specific GO:0001227 IDA
    DNA-binding transcription factor activity GO:0003700 TAS
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IPI
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IMP
    natural killer cell differentiation GO:0001779 ISS
    regulation of DNA-templated transcription GO:0006355 IBA
    regulation of transcription by RNA polymerase II GO:0006357 NAS
    transcription by RNA polymerase II GO:0006366 IEA
    immune response GO:0006955 IEA
    circadian rhythm GO:0007623 IBA
    positive regulation of gene expression GO:0010628 IEA
    negative regulation of DNA-templated transcription GO:0045892 IDA
    negative regulation of DNA-templated transcription GO:0045892 ISS
    positive regulation of DNA-templated transcription GO:0045893 ISS
    cellular response to interleukin-4 GO:0071353 IEA
Subcellular Localization
    chromatin GO:0000785 ISA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 NAS
    RNA polymerase II transcription regulator complex GO:0090575 IPI
 Experiment description of studies that identified NFIL3 in sEVs
1
Experiment ID 275
MISEV standards
EM
Biophysical techniques
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
AIF
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
 Protein-protein interactions for NFIL3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DNMT3L  
Reconstituted Complex Homo sapiens
2 MAFF 23764
FRET Homo sapiens
FRET Homo sapiens
3 TRAF2 7186
Two-hybrid Homo sapiens
4 RFWD2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 AMOTL2 51421
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
6 DR1  
Reconstituted Complex Homo sapiens
7 DEC1  
Two-hybrid Homo sapiens
8 PIH1D2  
Two-hybrid Homo sapiens
9 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
10 BATF  
FRET Homo sapiens
11 MAFG  
FRET Homo sapiens
12 NFIL3 4783
FRET Homo sapiens
FRET Homo sapiens
13 CREB1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 ATF1  
Affinity Capture-MS Homo sapiens
15 DDIT3 1649
FRET Homo sapiens
16 STK40  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 RINT1 60561
Two-hybrid Homo sapiens
18 HSF2BP  
Two-hybrid Homo sapiens
19 FUCA2 2519
Affinity Capture-MS Homo sapiens
20 PDE9A  
Two-hybrid Homo sapiens
21 CEBPA  
Proximity Label-MS Homo sapiens
22 SNX8 29886
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which NFIL3 is involved
PathwayEvidenceSource
Circadian Clock IEA Reactome





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