Gene description for CD40LG
Gene name CD40 ligand
Gene symbol CD40LG
Other names/aliases CD154
CD40L
HIGM1
IGM
IMD3
T-BAM
TNFSF5
TRAP
gp39
hCD40L
Species Homo sapiens
 Database cross references - CD40LG
ExoCarta ExoCarta_959
Entrez Gene 959
HGNC 11935
MIM 300386
UniProt P29965  
 CD40LG identified in exosomes derived from the following tissue/cell type
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for CD40LG
Molecular Function
    tumor necrosis factor receptor binding GO:0005164 IEA
    CD40 receptor binding GO:0005174 IPI
    cytokine activity GO:0005125 IEA
Biological Process
    B cell differentiation GO:0030183 IEA
    positive regulation of endothelial cell apoptotic process GO:2000353 IDA
    negative regulation of apoptotic process GO:0043066 IDA
    isotype switching GO:0045190 ISS
    leukocyte cell-cell adhesion GO:0007159 NAS
    inflammatory response GO:0006954 IDA
    regulation of immune response GO:0050776 TAS
    positive regulation of interleukin-12 production GO:0032735 IDA
    regulation of immunoglobulin secretion GO:0051023 IEA
    B cell proliferation GO:0042100 IDA
    platelet activation GO:0030168 IDA
    immunoglobulin secretion GO:0048305 IEA
Subcellular Localization
    external side of plasma membrane GO:0009897 IEA
    integral component of plasma membrane GO:0005887 TAS
    integral component of membrane GO:0016021 NAS
    plasma membrane GO:0005886 TAS
    extracellular space GO:0005615 IEA
 Experiment description of studies that identified CD40LG in exosomes
1
Experiment ID 231
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 232
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 233
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CD40LG
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CD40 958
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
2 CD40LG 959
Invitro Homo sapiens
Invitro Homo sapiens
3 CD5L 922
Invivo Homo sapiens
4 HPR 3250
Invitro Homo sapiens
View the network image/svg+xml
 Pathways in which CD40LG is involved
PathwayEvidenceSource
Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell TAS Reactome





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