Gene description for USP53
Gene name ubiquitin specific peptidase 53
Gene symbol USP53
Other names/aliases -
Species Homo sapiens
 Database cross references - USP53
ExoCarta ExoCarta_54532
Vesiclepedia VP_54532
Entrez Gene 54532
HGNC 29255
UniProt Q70EK8  
 USP53 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for USP53
Molecular Function
    cysteine-type deubiquitinase activity GO:0004843 IDA
    protein binding GO:0005515 IPI
Biological Process
    action potential GO:0001508 IEA
    sensory perception of sound GO:0007605 IBA
    response to auditory stimulus GO:0010996 IBA
    protein deubiquitination GO:0016579 IEA
    epithelial cell apoptotic process GO:1904019 IEA
    outer hair cell apoptotic process GO:1905584 IEA
Subcellular Localization
    cell-cell junction GO:0005911 IBA
    bicellular tight junction GO:0005923 IMP
 Experiment description of studies that identified USP53 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for USP53
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PSMA6 5687
Affinity Capture-MS Homo sapiens
2 KRT77 374454
Affinity Capture-MS Homo sapiens
3 CAT 847
Affinity Capture-MS Homo sapiens
4 CASP14 23581
Affinity Capture-MS Homo sapiens
5 PVRL2 5819
Two-hybrid Homo sapiens
6 TRAF2 7186
Two-hybrid Homo sapiens
7 YAE1D1  
Affinity Capture-MS Homo sapiens
8 GRB2 2885
Two-hybrid Homo sapiens
9 IREB2 3658
Two-hybrid Homo sapiens
10 PIP 5304
Affinity Capture-MS Homo sapiens
11 NINL  
Proximity Label-MS Homo sapiens
12 CRK 1398
Two-hybrid Homo sapiens
13 HCFC1 3054
Affinity Capture-MS Homo sapiens
14 CPNE7 27132
Affinity Capture-MS Homo sapiens
15 DSG1 1828
Affinity Capture-MS Homo sapiens
16 DAB1 1600
Two-hybrid Homo sapiens
17 DDB2  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
18 UBC 7316
Reconstituted Complex Homo sapiens
19 CSTA 1475
Affinity Capture-MS Homo sapiens
20 ARG1 383
Affinity Capture-MS Homo sapiens
21 AZGP1 563
Affinity Capture-MS Homo sapiens
22 RAD50 10111
Affinity Capture-MS Homo sapiens
23 SAMD4B  
Two-hybrid Homo sapiens
24 ANXA2P2 304
Affinity Capture-MS Homo sapiens
25 FKBP5 2289
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
26 PPP2R1B 5519
Affinity Capture-MS Homo sapiens
27 EZH2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
28 TGM3 7053
Affinity Capture-MS Homo sapiens
29 BLMH 642
Affinity Capture-MS Homo sapiens
30 FLNC 2318
Affinity Capture-MS Homo sapiens
31 BICD2 23299
Proximity Label-MS Homo sapiens
32 BICD1 636
Proximity Label-MS Homo sapiens
33 RPL26 6154
Affinity Capture-MS Homo sapiens
34 FBXO31 79791
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which USP53 is involved
No pathways found





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