Gene description for UAP1
Gene name UDP-N-acetylglucosamine pyrophosphorylase 1
Gene symbol UAP1
Other names/aliases AGX
AGX1
AGX2
SPAG2
Species Homo sapiens
 Database cross references - UAP1
ExoCarta ExoCarta_6675
Entrez Gene 6675
HGNC 12457
MIM 602862
UniProt Q16222  
 UAP1 identified in exosomes derived from the following tissue/cell type
Melanoma cells 25950383    
Melanoma cells 25950383    
 Gene ontology annotations for UAP1
Molecular Function
    UDP-N-acetylglucosamine diphosphorylase activity GO:0003977 TAS
    carbohydrate binding GO:0030246 IEA
    identical protein binding GO:0042802 IPI
Biological Process
    protein N-linked glycosylation via asparagine GO:0018279 TAS
    UDP-N-acetylglucosamine biosynthetic process GO:0006048 TAS
    cellular protein metabolic process GO:0044267 TAS
    dolichol-linked oligosaccharide biosynthetic process GO:0006488 TAS
    post-translational protein modification GO:0043687 TAS
Subcellular Localization
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
    nucleoplasm GO:0005654 IDA
 Experiment description of studies that identified UAP1 in exosomes
1
Experiment ID 259
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name A375M
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 260
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1|CD81
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 1205Lu
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for UAP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UAP1 6675
Affinity Capture-MS Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-MS Homo sapiens
Co-crystal Structure Homo sapiens
View the network image/svg+xml
 Pathways in which UAP1 is involved
PathwayEvidenceSource
Synthesis of UDP-N-acetyl-glucosamine TAS Reactome





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