Gene description for RFX5
Gene name regulatory factor X, 5 (influences HLA class II expression)
Gene symbol RFX5
Other names/aliases -
Species Homo sapiens
 Database cross references - RFX5
ExoCarta ExoCarta_5993
Vesiclepedia VP_5993
Entrez Gene 5993
HGNC 9986
MIM 601863
UniProt P48382  
 RFX5 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for RFX5
Molecular Function
    RNA polymerase II transcription regulatory region sequence-specific DNA binding GO:0000977 IDA
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IBA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 IBA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 ISA
    DNA-binding transcription activator activity, RNA polymerase II-specific GO:0001228 IDA
    DNA-binding transcription activator activity, RNA polymerase II-specific GO:0001228 IGI
    protein binding GO:0005515 IPI
    sequence-specific DNA binding GO:0043565 IDA
    sequence-specific double-stranded DNA binding GO:1990837 IDA
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IEA
    regulation of transcription by RNA polymerase II GO:0006357 IBA
    positive regulation of MHC class II biosynthetic process GO:0045348 NAS
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
Subcellular Localization
    chromatin GO:0000785 ISA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    intracellular membrane-bounded organelle GO:0043231 IDA
    RNA polymerase II transcription regulator complex GO:0090575 IPI
 Experiment description of studies that identified RFX5 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for RFX5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MTHFD2 10797
Co-fractionation Homo sapiens
2 SLIRP 81892
Co-fractionation Homo sapiens
3 HDAC4  
Affinity Capture-Western Homo sapiens
4 PAX6  
Proximity Label-MS Homo sapiens
5 GIGYF2 26058
Affinity Capture-MS Homo sapiens
6 MLLT1 4298
Affinity Capture-MS Homo sapiens
7 ANKRA2  
Protein-peptide Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 HDAC2 3066
Affinity Capture-Western Homo sapiens
9 PARP1 142
Proximity Label-MS Homo sapiens
10 FEV  
Proximity Label-MS Homo sapiens
11 CDC73  
Affinity Capture-MS Homo sapiens
12 RFX7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 CSTF2T  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 ECT2 1894
Affinity Capture-MS Homo sapiens
15 PPARG 5468
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
16 RFXAP  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 HIST1H2BG 8339
Proximity Label-MS Homo sapiens
18 MRPL53  
Co-fractionation Homo sapiens
19 RPS19 6223
Co-fractionation Homo sapiens
20 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
21 RBMS1 5937
Co-fractionation Homo sapiens
22 SRPRB 58477
Co-fractionation Homo sapiens
23 CIITA  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Far Western Homo sapiens
Reconstituted Complex Homo sapiens
24 APP 351
Reconstituted Complex Homo sapiens
25 NXF1 10482
Affinity Capture-RNA Homo sapiens
26 NFIX 4784
Proximity Label-MS Homo sapiens
27 APEX1 328
Affinity Capture-RNA Homo sapiens
28 LSM7  
Co-fractionation Homo sapiens
29 TRIM37  
Proximity Label-MS Homo sapiens
30 NDUFA2 4695
Co-fractionation Homo sapiens
31 RPA3 6119
Proximity Label-MS Homo sapiens
32 HSPA2 3306
Affinity Capture-MS Homo sapiens
33 KIF23 9493
Affinity Capture-MS Homo sapiens
34 NDUFA7 4701
Co-fractionation Homo sapiens
35 RFXANK  
Two-hybrid Homo sapiens
Protein-peptide Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 CDV3 55573
Co-fractionation Homo sapiens
37 MOV10 4343
Affinity Capture-RNA Homo sapiens
38 NFYC 4802
Proximity Label-MS Homo sapiens
39 AR 367
Proximity Label-MS Homo sapiens
40 CEBPA  
Proximity Label-MS Homo sapiens
41 U2SURP 23350
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which RFX5 is involved
No pathways found





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