Gene description for SPPL2A
Gene name signal peptide peptidase like 2A
Gene symbol SPPL2A
Other names/aliases IMP3
PSL2
Species Homo sapiens
 Database cross references - SPPL2A
ExoCarta ExoCarta_84888
Entrez Gene 84888
HGNC 30227
MIM 608238
UniProt Q8TCT8  
 SPPL2A identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for SPPL2A
Molecular Function
    protein homodimerization activity GO:0042803 IDA
    aspartic-type endopeptidase activity GO:0004190 IEA
    protein binding GO:0005515 IPI
Biological Process
    membrane protein ectodomain proteolysis GO:0006509 IDA
    regulation of immune response GO:0050776 IMP
    membrane protein intracellular domain proteolysis GO:0031293 IMP
Subcellular Localization
    integral component of cytoplasmic side of endoplasmic reticulum membrane GO:0071458 IDA
    plasma membrane GO:0005886 IEA
    late endosome membrane GO:0031902 IEA
    lysosomal membrane GO:0005765 IDA
    extracellular exosome GO:0070062 IDA
    Golgi-associated vesicle membrane GO:0030660 IDA
    integral component of lumenal side of endoplasmic reticulum membrane GO:0071556 IDA
 Experiment description of studies that identified SPPL2A in exosomes
1
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SPPL2A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SGTA 6449
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which SPPL2A is involved
No pathways found





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