Gene description for MFSD8
Gene name major facilitator superfamily domain containing 8
Gene symbol MFSD8
Other names/aliases CCMD
CLN7
Species Homo sapiens
 Database cross references - MFSD8
ExoCarta ExoCarta_256471
Vesiclepedia VP_256471
Entrez Gene 256471
HGNC 28486
MIM 611124
UniProt Q8NHS3  
 MFSD8 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for MFSD8
Molecular Function
    chloride channel activity GO:0005254 IBA
    chloride channel activity GO:0005254 IDA
    iodide transmembrane transporter activity GO:0015111 IBA
    iodide transmembrane transporter activity GO:0015111 IDA
    fluoride channel activity GO:0062054 IDA
Biological Process
    glycolytic process GO:0006096 IEA
    glycolipid metabolic process GO:0006664 IEA
    mitochondrion organization GO:0007005 IEA
    lysosome organization GO:0007040 IBA
    determination of adult lifespan GO:0008340 IEA
    glycoprotein metabolic process GO:0009100 IEA
    gene expression GO:0010467 IEA
    regulation of autophagy GO:0010506 IEA
    microglia differentiation GO:0014004 IEA
    iodide transport GO:0015705 IEA
    multicellular organism growth GO:0035264 IEA
    TORC1 signaling GO:0038202 IEA
    negative regulation of neuron apoptotic process GO:0043524 IEA
    cellular respiration GO:0045333 IEA
    neuron development GO:0048666 IEA
    astrocyte differentiation GO:0048708 IEA
    protein stabilization GO:0050821 IEA
    neuromuscular process GO:0050905 IEA
    maintenance of location GO:0051235 IEA
    neuron apoptotic process GO:0051402 IEA
    retina development in camera-type eye GO:0060041 IEA
    motor behavior GO:0061744 IEA
    inclusion body assembly GO:0070841 IEA
    reactive oxygen species metabolic process GO:0072593 IEA
    autophagosome maturation GO:0097352 IBA
    chloride transmembrane transport GO:1902476 IEA
    fluoride transmembrane transport GO:1903424 IEA
    lysosomal protein catabolic process GO:1905146 IEA
    regulation of lysosomal protein catabolic process GO:1905165 IEA
Subcellular Localization
    mitochondrion GO:0005739 IEA
    lysosomal membrane GO:0005765 HDA
    lysosomal membrane GO:0005765 IBA
    lysosomal membrane GO:0005765 IDA
    endosome membrane GO:0010008 IDA
    chloride channel complex GO:0034707 IEA
 Experiment description of studies that identified MFSD8 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for MFSD8
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TACR3  
Affinity Capture-MS Homo sapiens
2 TPRA1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 SLC43A3 29015
Affinity Capture-MS Homo sapiens
4 SLC22A16 85413
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 PTGIR  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 C4orf32  
Affinity Capture-MS Homo sapiens
7 CXCR4 7852
Affinity Capture-MS Homo sapiens
8 DNAJC19 131118
Affinity Capture-MS Homo sapiens
9 ENDOD1 23052
Affinity Capture-MS Homo sapiens
10 GPR17 2840
Affinity Capture-MS Homo sapiens
11 LAMTOR1 55004
Proximity Label-MS Homo sapiens
12 LAMP3  
Proximity Label-MS Homo sapiens
13 C3AR1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 S1PR2 9294
Affinity Capture-MS Homo sapiens
15 TNFSF8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 CD53 963
Affinity Capture-MS Homo sapiens
17 CAV2 858
Affinity Capture-MS Homo sapiens
18 CYB5R1 51706
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 ASGR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 KLRC4  
Affinity Capture-MS Homo sapiens
21 BSCL2  
Affinity Capture-MS Homo sapiens
22 TSPAN15 23555
Affinity Capture-MS Homo sapiens
23 EPHX1 2052
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 LAMP2 3920
Proximity Label-MS Homo sapiens
25 KDSR 2531
Affinity Capture-MS Homo sapiens
26 GPR114  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 OPRL1 4987
Affinity Capture-MS Homo sapiens
28 C5AR1  
Affinity Capture-MS Homo sapiens
29 SLC22A9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 FZD10  
Affinity Capture-MS Homo sapiens
31 ZACN  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 KRTCAP3 200634
Affinity Capture-MS Homo sapiens
33 SLC18A2  
Affinity Capture-MS Homo sapiens
34 PDE3A  
Affinity Capture-MS Homo sapiens
35 HLA-DQA1 3117
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 IL7R  
Affinity Capture-MS Homo sapiens
37 RAB2A 5862
Proximity Label-MS Homo sapiens
38 HTR2C  
Affinity Capture-MS Homo sapiens
39 GPR88  
Affinity Capture-MS Homo sapiens
40 SPPL2B 56928
Affinity Capture-MS Homo sapiens
41 CMTM5  
Affinity Capture-MS Homo sapiens
42 RAB9A 9367
Proximity Label-MS Homo sapiens
43 GPR21  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 GABBR1  
Affinity Capture-MS Homo sapiens
45 PTPRN  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 IPPK  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 ADGRE5 976
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 YIPF3 25844
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 CCR6  
Affinity Capture-MS Homo sapiens
50 SLC7A1 6541
Affinity Capture-MS Homo sapiens
51 SLC22A4 6583
Affinity Capture-MS Homo sapiens
52 FPR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 EBP  
Affinity Capture-MS Homo sapiens
54 LPAR1 1902
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 P2RY12 64805
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 CACNG1  
Affinity Capture-MS Homo sapiens
57 AVPR2  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which MFSD8 is involved
No pathways found





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