Gene description for NDFIP2
Gene name Nedd4 family interacting protein 2
Gene symbol NDFIP2
Other names/aliases N4WBP5A
Species Homo sapiens
 Database cross references - NDFIP2
ExoCarta ExoCarta_54602
Vesiclepedia VP_54602
Entrez Gene 54602
HGNC 18537
MIM 610041
UniProt Q9NV92  
 NDFIP2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for NDFIP2
Molecular Function
    protein binding GO:0005515 IPI
    WW domain binding GO:0050699 IPI
Biological Process
    ubiquitin-dependent protein catabolic process GO:0006511 IBA
    vacuolar transport GO:0007034 IEA
    negative regulation of gene expression GO:0010629 IMP
    metal ion transport GO:0030001 IEA
    positive regulation of protein ubiquitination GO:0031398 IBA
    positive regulation of protein ubiquitination GO:0031398 IMP
    negative regulation of transporter activity GO:0032410 IMP
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 HMP
    negative regulation of protein transport GO:0051224 IMP
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    cytoplasm GO:0005737 IDA
    mitochondrion GO:0005739 IDA
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum GO:0005783 IDA
    Golgi apparatus GO:0005794 IBA
    Golgi apparatus GO:0005794 IDA
    multivesicular body membrane GO:0032585 IEA
    intracellular membrane-bounded organelle GO:0043231 IDA
    perinuclear region of cytoplasm GO:0048471 IBA
    perinuclear region of cytoplasm GO:0048471 IDA
 Experiment description of studies that identified NDFIP2 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for NDFIP2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RNF149 284996
Affinity Capture-MS Homo sapiens
2 NDUFAF1  
Affinity Capture-MS Homo sapiens
3 LRP10 26020
Affinity Capture-MS Homo sapiens
4 RABAC1 10567
Affinity Capture-MS Homo sapiens
5 AQP2 359
Two-hybrid Homo sapiens
6 NEDD4 4734
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
7 ZDHHC9  
Affinity Capture-MS Homo sapiens
8 DDAH1 23576
Affinity Capture-MS Homo sapiens
9 WWP1 11059
Affinity Capture-MS Homo sapiens
10 MARCH1  
Affinity Capture-MS Homo sapiens
11 SDHA 6389
Affinity Capture-MS Homo sapiens
12 NRSN1  
Affinity Capture-MS Homo sapiens
13 HCST  
Affinity Capture-MS Homo sapiens
14 MFSD4  
Affinity Capture-MS Homo sapiens
15 NDFIP1 80762
Affinity Capture-MS Homo sapiens
16 IL20RB  
Affinity Capture-MS Homo sapiens
17 IL2RG  
Affinity Capture-MS Homo sapiens
18 TMEM245 23731
Affinity Capture-MS Homo sapiens
19 PTEN 5728
Affinity Capture-Western Homo sapiens
20 FAM168A  
Affinity Capture-MS Homo sapiens
21 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
22 TSPAN3 10099
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 TBXA2R 6915
Affinity Capture-MS Homo sapiens
24 LYN 4067
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
25 KCNK18  
Affinity Capture-MS Homo sapiens
26 WWP2 11060
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
27 SLC11A2 4891
Affinity Capture-Western Homo sapiens
28 YAP1 10413
Affinity Capture-MS Homo sapiens
29 EGFR 1956
Affinity Capture-Western Homo sapiens
30 SAR1B 51128
Affinity Capture-MS Homo sapiens
31 FAM8A1 51439
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 TMEM231 79583
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 SRC 6714
Affinity Capture-Western Homo sapiens
34 PTGFR 5737
Affinity Capture-MS Homo sapiens
35 FYN 2534
Affinity Capture-Western Homo sapiens
36 UQCRH 7388
Two-hybrid Homo sapiens
37 ITCH 83737
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
38 NEDD4L 23327
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Far Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
39 DGCR2 9993
Affinity Capture-MS Homo sapiens
40 EDAR  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which NDFIP2 is involved
No pathways found





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