Gene description for TMLHE
Gene name trimethyllysine hydroxylase, epsilon
Gene symbol TMLHE
Other names/aliases AUTSX6
BBOX2
TMLD
TMLH
TMLHED
XAP130
Species Homo sapiens
 Database cross references - TMLHE
ExoCarta ExoCarta_55217
Vesiclepedia VP_55217
Entrez Gene 55217
HGNC 18308
MIM 300777
UniProt Q9NVH6  
 TMLHE identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for TMLHE
Molecular Function
    iron ion binding GO:0005506 IEA
    trimethyllysine dioxygenase activity GO:0050353 EXP
    trimethyllysine dioxygenase activity GO:0050353 IDA
    trimethyllysine dioxygenase activity GO:0050353 IDA
    trimethyllysine dioxygenase activity GO:0050353 IMP
Biological Process
    carnitine biosynthetic process GO:0045329 IBA
    carnitine biosynthetic process GO:0045329 IDA
    carnitine biosynthetic process GO:0045329 IEA
    carnitine biosynthetic process GO:0045329 IMP
    carnitine biosynthetic process GO:0045329 TAS
    negative regulation of oxidoreductase activity GO:0051354 IDA
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    mitochondrial matrix GO:0005759 TAS
 Experiment description of studies that identified TMLHE in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for TMLHE
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NIPSNAP3A  
Affinity Capture-MS Homo sapiens
2 CLPP 8192
Proximity Label-MS Homo sapiens
3 UQCRFS1 7386
Affinity Capture-MS Homo sapiens
4 LDHAL6B 92483
Affinity Capture-MS Homo sapiens
5 PNPLA4  
Affinity Capture-MS Homo sapiens
6 PCK1 5105
Affinity Capture-MS Homo sapiens
7 AK4 205
Affinity Capture-MS Homo sapiens
8 C6orf57  
Affinity Capture-MS Homo sapiens
9 NAA38  
Affinity Capture-MS Homo sapiens
10 MRPS2 51116
Affinity Capture-MS Homo sapiens
11 KLK5 25818
Affinity Capture-MS Homo sapiens
12 RPS21 6227
Affinity Capture-MS Homo sapiens
13 ARHGEF26 26084
Affinity Capture-MS Homo sapiens
14 DNAJA2 10294
Affinity Capture-MS Homo sapiens
15 METTL20  
Affinity Capture-MS Homo sapiens
16 ACAA2 10449
Affinity Capture-MS Homo sapiens
17 PDHA1 5160
Proximity Label-MS Homo sapiens
18 ISCA1  
Affinity Capture-MS Homo sapiens
19 CUL3 8452
Affinity Capture-MS Homo sapiens
20 ACSM5 54988
Affinity Capture-MS Homo sapiens
21 BTG3  
Affinity Capture-MS Homo sapiens
22 NDUFS7 374291
Affinity Capture-MS Homo sapiens
23 FAHD1 81889
Affinity Capture-MS Homo sapiens
24 MYL10 93408
Affinity Capture-MS Homo sapiens
25 TMLHE 55217
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
26 NIPSNAP3B 55335
Affinity Capture-MS Homo sapiens
27 AMACR 23600
Affinity Capture-MS Homo sapiens
28 GAPDHS 26330
Affinity Capture-MS Homo sapiens
29 DHRS2 10202
Affinity Capture-MS Homo sapiens
30 WWP2 11060
Affinity Capture-MS Homo sapiens
31 SSC4D  
Affinity Capture-MS Homo sapiens
32 TRMU  
Affinity Capture-MS Homo sapiens
33 GPR45  
Affinity Capture-MS Homo sapiens
34 RPUSD3  
Affinity Capture-MS Homo sapiens
35 STIM2 57620
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 DNPEP 23549
Affinity Capture-MS Homo sapiens
37 FAM174A 345757
Affinity Capture-MS Homo sapiens
38 YARS2  
Affinity Capture-MS Homo sapiens
39 MRPS24 64951
Affinity Capture-MS Homo sapiens
40 OXCT2  
Affinity Capture-MS Homo sapiens
41 CSNK1E 1454
Affinity Capture-MS Homo sapiens
42 FTSJ2 29960
Affinity Capture-MS Homo sapiens
43 MRPS17 51373
Affinity Capture-MS Homo sapiens
44 RASL10B  
Affinity Capture-MS Homo sapiens
45 GBAS 2631
Affinity Capture-MS Homo sapiens
46 TRIM43  
Affinity Capture-MS Homo sapiens
47 EXD2  
Proximity Label-MS Homo sapiens
48 TULP3 7289
Affinity Capture-MS Homo sapiens
49 SLC25A10 1468
Affinity Capture-MS Homo sapiens
50 SHC2 25759
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which TMLHE is involved
PathwayEvidenceSource
Carnitine synthesis TAS Reactome
Metabolism TAS Reactome
Metabolism of amino acids and derivatives TAS Reactome





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