Gene description for Scai
Gene name suppressor of cancer cell invasion
Gene symbol Scai
Other names/aliases 9330112M16
A930041I02Rik
AI662729
Species Mus musculus
 Database cross references - Scai
ExoCarta ExoCarta_320271
Vesiclepedia VP_320271
Entrez Gene 320271
UniProt Q8C8N2  
 Scai identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Scai
Molecular Function
    transcription corepressor activity GO:0003714 IBA
    transcription corepressor activity GO:0003714 IDA
    transcription corepressor activity GO:0003714 ISO
    protein binding GO:0005515 IPI
Biological Process
    DNA-templated transcription GO:0006351 IEA
    negative regulation of cell migration GO:0030336 IGI
    negative regulation of cell migration GO:0030336 ISO
    negative regulation of Rho protein signal transduction GO:0035024 IDA
    negative regulation of DNA-templated transcription GO:0045892 IEA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 IDA
    nuclear membrane GO:0031965 IEA
    nuclear membrane GO:0031965 ISO
 Experiment description of studies that identified Scai in exosomes
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Scai
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DOT1L 84444
Affinity Capture-MS Homo sapiens
2 PPFIBP1 8496
Affinity Capture-MS Homo sapiens
3 Kctd13  
Affinity Capture-MS Mus musculus
4 Cbx1  
Affinity Capture-MS Mus musculus
5 CRY2  
Affinity Capture-MS Homo sapiens
6 Cbx5  
Affinity Capture-MS Mus musculus
7 MLLT10  
Affinity Capture-MS Homo sapiens
8 PFKL 5211
Affinity Capture-MS Homo sapiens
9 ICE2  
Affinity Capture-MS Homo sapiens
10 SDF4 51150
Affinity Capture-MS Homo sapiens
11 EVI5L  
Affinity Capture-MS Homo sapiens
12 RAD51  
Affinity Capture-MS Homo sapiens
13 AHDC1  
Affinity Capture-MS Homo sapiens
14 TP53BP1 7158
Affinity Capture-MS Homo sapiens
15 CBX5 23468
Affinity Capture-MS Homo sapiens
16 SDCCAG3  
Affinity Capture-MS Homo sapiens
17 BCR 613
Affinity Capture-MS Homo sapiens
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