Gene description for BCHE
Gene name butyrylcholinesterase
Gene symbol BCHE
Other names/aliases CHE1
CHE2
E1
Species Homo sapiens
 Database cross references - BCHE
ExoCarta ExoCarta_590
Vesiclepedia VP_590
Entrez Gene 590
HGNC 983
MIM 177400
UniProt P06276  
 BCHE identified in exosomes derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Plasma 36624553    
Plasma 36624553    
Prostate cancer cells 22723089    
 Gene ontology annotations for BCHE
Molecular Function
    choline binding GO:0033265 IEA
    catalytic activity GO:0003824 NAS
    enzyme binding GO:0019899 NAS
    cholinesterase activity GO:0004104 IDA
    beta-amyloid binding GO:0001540 NAS
    identical protein binding GO:0042802 IPI
    acetylcholinesterase activity GO:0003990 ISS
Biological Process
    response to glucocorticoid GO:0051384 IEA
    negative regulation of synaptic transmission GO:0050805 IEA
    choline metabolic process GO:0019695 IEA
    cellular protein metabolic process GO:0044267 TAS
    response to alkaloid GO:0043279 IEA
    response to drug GO:0042493 IEA
    learning GO:0007612 IEA
    cocaine metabolic process GO:0050783 TAS
    response to folic acid GO:0051593 IEA
    neuroblast differentiation GO:0014016 IEA
    negative regulation of cell proliferation GO:0008285 IEA
Subcellular Localization
    membrane GO:0016020 IEA
    endoplasmic reticulum lumen GO:0005788 IEA
    blood microparticle GO:0072562 IDA
    nuclear envelope lumen GO:0005641 IEA
    extracellular space GO:0005615 IBA
    extracellular region GO:0005576 TAS
 Experiment description of studies that identified BCHE in exosomes
1
Experiment ID 497
MISEV standards
✘ EV Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD.
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 498
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD.
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 606
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|HSP90|TSG101|GAPDH
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Western blotting
PubMed ID 36624553    
Organism Homo sapiens
Experiment description Combination of size-exclusion chromatography and ion exchange adsorption for improving the proteomic analysis of plasma-derived extracellular vesicles
Authors Wang Y, Zhang Y, Li Z, Wei S, Chi X, Yan X, Lv H, Zhao L, Zhao L.
Journal name Proteomics
Publication year 2023
Sample Plasma
Sample name Plasma - Ion exchange adsorption 12.5 mg
Isolation/purification methods Filtration
Size exclusion chromatography
Ion exchange adsorption
Centrifugation
Centrifugal ultrafiltration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 606
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|HSP90|TSG101|GAPDH
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36624553    
Organism Homo sapiens
Experiment description Combination of size-exclusion chromatography and ion exchange adsorption for improving the proteomic analysis of plasma-derived extracellular vesicles
Authors Wang Y, Zhang Y, Li Z, Wei S, Chi X, Yan X, Lv H, Zhao L, Zhao L.
Journal name Proteomics
Publication year 2023
Sample Plasma
Sample name Plasma - Ion exchange adsorption 12.5 mg
Isolation/purification methods Filtration
Size exclusion chromatography
Ion exchange adsorption
Centrifugation
Centrifugal ultrafiltration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 607
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|HSP90|TSG101|GAPDH
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Western blotting
PubMed ID 36624553    
Organism Homo sapiens
Experiment description Combination of size-exclusion chromatography and ion exchange adsorption for improving the proteomic analysis of plasma-derived extracellular vesicles
Authors Wang Y, Zhang Y, Li Z, Wei S, Chi X, Yan X, Lv H, Zhao L, Zhao L.
Journal name Proteomics
Publication year 2023
Sample Plasma
Sample name Plasma - Ion exchange adsorption 25 mg
Isolation/purification methods Filtration
Size exclusion chromatography
Ion exchange adsorption
Centrifugation
Centrifugal ultrafiltration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 607
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|HSP90|TSG101|GAPDH
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36624553    
Organism Homo sapiens
Experiment description Combination of size-exclusion chromatography and ion exchange adsorption for improving the proteomic analysis of plasma-derived extracellular vesicles
Authors Wang Y, Zhang Y, Li Z, Wei S, Chi X, Yan X, Lv H, Zhao L, Zhao L.
Journal name Proteomics
Publication year 2023
Sample Plasma
Sample name Plasma - Ion exchange adsorption 25 mg
Isolation/purification methods Filtration
Size exclusion chromatography
Ion exchange adsorption
Centrifugation
Centrifugal ultrafiltration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 143
MISEV standards
✔
EM
EV Biophysical techniques
✔
HSP70|HSP90|RAB5|LAMP2|CD9
EV Enriched markers
✔
HSP90B1
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors Hosseini-Beheshti E, Guns ES.
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name LNCaP - Rep 3
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
 Protein-protein interactions for BCHE
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 COLQ  
Invitro Homo sapiens
View the network image/svg+xml



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