Experiment description of studies that identified DCLK2 in exosomes

1 Experiment ID 237 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocytes Sample name MIHA Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RNA Sequencing

2 Experiment ID 126 MISEV standards ✘ Biophysical techniques ✔ GAPDH Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry [LTQ-FT Ultra] PubMed ID Unpublished / Not applicable Organism Homo sapiens Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy? Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim" Journal name Mesenchymal Stem Cell Therapy Publication year 2011 Sample Mesenchymal stem cells Sample name huES9.E1 Isolation/purification methods HPLC Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Antobody array Mass spectrometry

Protein-protein interactions for DCLK2

Protein Interactor ExoCarta ID Identification method PubMed Species 1 DCTN1 1639 Proximity Label-MS Homo sapiens 2 CCDC57 284001 Two-hybrid Homo sapiens 3 FBLIM1 54751 Affinity Capture-MS Homo sapiens 4 ENO1 2023 Affinity Capture-RNA Homo sapiens 5 APEX1 328 Affinity Capture-RNA Homo sapiens 6 KLHL15   Affinity Capture-Western Homo sapiens Affinity Capture-Western Homo sapiens 7 TSC22D3   Two-hybrid Homo sapiens 8 HSP90AA1 3320 Affinity Capture-Luminescence Homo sapiens View the network

Pathways in which DCLK2 is involved

No pathways found