Gene description for SCAF8
Gene name SR-related CTD-associated factor 8
Gene symbol SCAF8
Other names/aliases RBM16
Species Homo sapiens
 Database cross references - SCAF8
ExoCarta ExoCarta_22828
Vesiclepedia VP_22828
Entrez Gene 22828
HGNC 20959
MIM 616024
UniProt Q9UPN6  
 SCAF8 identified in exosomes derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
 Gene ontology annotations for SCAF8
Molecular Function
    RNA polymerase II complex binding GO:0000993 IBA
    RNA binding GO:0003723 IDA
    mRNA binding GO:0003729 IBA
    protein binding GO:0005515 IPI
    RNA polymerase core enzyme binding GO:0043175 IPI
    RNA polymerase II C-terminal domain phosphoserine binding GO:1990269 IDA
Biological Process
    termination of RNA polymerase II transcription GO:0006369 IBA
    positive regulation of DNA-templated transcription, elongation GO:0032786 IDA
    negative regulation of termination of RNA polymerase II transcription, poly(A)-coupled GO:2000805 IDA
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IBA
    mRNA cleavage factor complex GO:0005849 IBA
    nuclear matrix GO:0016363 ISS
 Experiment description of studies that identified SCAF8 in exosomes
1
Experiment ID 497
MISEV standards
Biophysical techniques
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 498
MISEV standards
EM
Biophysical techniques
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for SCAF8
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 POLR2C 5432
Proximity Label-MS Homo sapiens
2 CALML3 810
Affinity Capture-MS Homo sapiens
3 NAGK 55577
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 NTRK1 4914
Affinity Capture-MS Homo sapiens
5 HRASLS2  
Affinity Capture-MS Homo sapiens
6 GIGYF1  
Two-hybrid Homo sapiens
7 MMGT1 93380
Affinity Capture-MS Homo sapiens
8 WWOX 51741
Affinity Capture-MS Homo sapiens
9 E2F4  
Affinity Capture-MS Homo sapiens
10 TEX11  
Two-hybrid Homo sapiens
11 JMJD6 23210
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 DTX2 113878
Proximity Label-MS Homo sapiens
13 HNRNPA1 3178
Proximity Label-MS Homo sapiens
14 SHMT2 6472
Affinity Capture-RNA Homo sapiens
15 NUP35 129401
Proximity Label-MS Homo sapiens
16 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
17 MYO1C 4641
Affinity Capture-MS Homo sapiens
18 NXF1 10482
Affinity Capture-RNA Homo sapiens
19 GOLGA2 2801
Two-hybrid Homo sapiens
20 UFL1 23376
Affinity Capture-MS Homo sapiens
21 IDE 3416
Affinity Capture-MS Homo sapiens
22 TRIM37  
Proximity Label-MS Homo sapiens
23 MOV10 4343
Affinity Capture-RNA Homo sapiens
24 EMC2 9694
Affinity Capture-MS Homo sapiens
25 INO80B 83444
Affinity Capture-MS Homo sapiens
26 DNPEP 23549
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 KLHL22 84861
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 Junb  
Affinity Capture-MS Mus musculus
29 ENO1 2023
Affinity Capture-RNA Homo sapiens
30 DDRGK1 65992
Affinity Capture-MS Homo sapiens
31 FAM188A 80013
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 MTUS2 23281
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 SP7  
Proximity Label-MS Homo sapiens
34 THRSP  
Affinity Capture-MS Homo sapiens
35 Cbx4  
Affinity Capture-MS Mus musculus
36 CDC5L 988
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
Affinity Capture-MS Homo sapiens
37 HMGB2 3148
Co-fractionation Homo sapiens
View the network image/svg+xml
 Pathways in which SCAF8 is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here